We narrowed to 16,278 results for: nes

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pDGE79

Plasmid

#80581

Purpose

recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, BASTA

Depositor

Johannes Stuttmann

Publication

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Inserts

pnos:PAT-tnos
pPcUbi:Cas9 D10A-tnos
BsaI-ccdB_CmR-BsaI

Use

CRISPR

Expression

Plant

Mutation

D10A

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pFoxd3IRESGFPtv

Plasmid

#37266

Depositor

Patricia Labosky

Publication

Hanna et al Genes Dev. 2002 Oct 15;16(20):2650-61.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Foxd3 (Foxd3 Mouse)

Use

Mouse Targeting

Tags

IRES-GFP

Available since

Aug. 1, 2012

Availability

Academic Institutions and Nonprofits only
A337C

Plasmid

#24432

Depositor

Ron Vale

Publication

Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

Insert

Kinesin (KIF5B Human)

Tags

6XHis and GFP

Expression

Bacterial

Mutation

C7S/C65A/C168A/C174S/C294A/C330S/C421A, A337C

Available since

June 14, 2010

Availability

Academic Institutions and Nonprofits only
AAVS1_dCas9-XTEN-KRAB-p2A-GFP

Plasmid

#222108

Purpose

Donor vector for CRISPRi integration at AAVS1 with a GFP fluorophore

Depositor

Jonathan Weissman

Publication

She et al Cell. 2023 Jul 6;186(14):2977-2994.e23.

Insert

CRISPRi-kox1(KRAB) (cas9 S. pyogenes, Human)

Use

AAV and CRISPR

Tags

HA and P2A-GFP

Expression

Mammalian

Mutation

D10A, H840A

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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PET-B-HypaR-SpCas9-NLS-6xHis

Plasmid

#207388

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Publication

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HypaR-SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

R661A, N692A, M694A, Q695A, H698A, amino acids 10…

Promoter

T7

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMMACE DEST CMV SpCas9

Plasmid

#206268

Purpose

DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes SpCas9 under the control of CMV promoter. CRE-Acceptor in MultiBac system.

Depositor

Imre Berger

Publication

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SpCas9

Use

CRISPR; Recombinant baculovirus production

Expression

Mammalian

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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PET-B-SpCas9-HF1-NLS-6xHis

Plasmid

#207385

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Publication

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-SpCas9-HF1

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

N497A, R661A, Q695A, Q926A, amino acids 1005-1013…

Promoter

T7

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hWWTR1-W2B)-PGKpuro2AmCherry-W

Plasmid

#163177

Purpose

Lentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of mCherry tag

Depositor

Sok Ching Cheong

Publication

Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

Insert

WWTR1 (WWTR1 Human)

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

U6

Available since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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Cas9_YTHDF2_sgRNA

Plasmid

#186673

Purpose

YTHDF2 sgRNA plasmid

Depositor

Astrid Haase

Publication

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

YTHDF2 KO sgRNA Plasmid (YTHDF2 Human)

Expression

Mammalian

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-2)

Plasmid

#159660

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.

Depositor

Joseph Brzezinski

Publication

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hSpCas9

Use

CRISPR

Promoter

Ef1a

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-3)

Plasmid

#159661

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.

Depositor

Joseph Brzezinski

Publication

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hSpCas9

Use

CRISPR

Promoter

Ef1a

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-1)

Plasmid

#159659

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.

Depositor

Joseph Brzezinski

Publication

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hSpCas9

Use

CRISPR

Promoter

Ef1a

Available since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCSD_2XNLS-SpCas9MT3-NLS-3xHA-2XNLS-NmCas9WT-2XNLS

Plasmid

#107325

Purpose

Expresses SpCas9 (R1335K) fused to NmCas9 in mammalian cells

Depositor

Scot Wolfe

Publication

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9-NmCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 (R1335K) is fused to NmCas9

Promoter

CMV IE94

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCSD_2XNLS-SpCas9MT2-NLS-3xHA-2XNLS-NmCas9WT-2XNLS

Plasmid

#107326

Purpose

Expresses SpCas9 (R1333S) fused to NmCas9 in mammalian cells

Depositor

Scot Wolfe

Publication

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9-NmCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 (R1333S) is fused to NmCas9

Promoter

CMV IE93

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-MKLP1 S710A

Plasmid

#70149

Purpose

mammalian expression of MKLP1 with an eGFP fusion and an S710A mutation

Depositor

Masanori Mishima

Publication

Douglas et al Curr Biol. 2010 May 25;20(10):927-33. do

Insert

MKLP1 (KIF23 Human)

Tags

eGFP

Expression

Mammalian

Mutation

S710A

Promoter

CMV

Available since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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K349CLM-6His 215/347

Plasmid

#24451

Depositor

Ron Vale

Publication

Tomishige et al Nat Struct Mol Biol. 2006 Oct . 13(10):8

Insert

Kinesin (KIF5B Human)

Tags

His

Expression

Bacterial

Mutation

C7S/C65A/C168A/C174S/C294A/C330S, E215C, E347C

Available since

July 15, 2010

Availability

Academic Institutions and Nonprofits only
K222C/E334C

Plasmid

#24435

Depositor

Ron Vale

Publication

Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

Insert

Kinesin (KIF5B Human)

Tags

6XHis and GFP

Expression

Bacterial

Mutation

C7S/C65A/C168A/C174S/C294A/C330S/C421A, K222C/E…

Available since

July 13, 2010

Availability

Academic Institutions and Nonprofits only
A337C/Y344C

Plasmid

#24434

Depositor

Ron Vale

Publication

Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

Insert

Kinesin (KIF5B Human)

Tags

6XHis and GFP

Expression

Bacterial

Mutation

C7S/C65A/C168A/C174S/C294A/C330S/C421A, A337C/Y…

Available since

June 14, 2010

Availability

Academic Institutions and Nonprofits only
CLM RP KGF Mut 1

Plasmid

#24166

Depositor

Ron Vale

Publication

Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

Insert

Kinesin (KIF5B Human)

Tags

6XHis and GFP

Expression

Bacterial

Mutation

C7S/C65A/C168A/C174S/C294A/C330S/C421A

Available since

May 5, 2010

Availability

Academic Institutions and Nonprofits only
pML104-KanMX-sgRNAv2

Plasmid

#254712

Purpose

Yeast CRISPR-Cas9 backbone with sgRNA cassette for rapid PCR-based guide insertion; KanMX/G418 selection in S. cerevisiae, Amp/Kan propagation in E. coli.

Depositor

Brian Strahl

Publication

Rostamian et al STAR Protoc. 2026 Jul 28;7(3):104751. do

Insert

YKU70 sgRNA (YKU70 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

SNR52 promoter

Available since

Aug. 18, 2026

Availability

Academic Institutions and Nonprofits only

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