We narrowed to 19,051 results for: Tec

1. B-Sniper SpCas9

   Plasmid

   #207361

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-Sniper SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   F539S, M763I, K890N, amino acids 1005-1013 replac…

   Promoter

   CBh

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)-HLA-cmyc-optiT1R3 a21-852

   Plasmid

   #113949

   Purpose

   mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R3 (TAS1R3 Human)
   

   Tags

   Signal/leader sequence from HLA class I histocomp…

   Expression

   Mammalian

   Mutation

   residues 21-852

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-cFos-XRI-V5

   Plasmid

   #178058

   Purpose

   Expression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with V5 tag, under c-fos promoter.

   Depositor

   Edward Boyden

   Article

   Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   XRI-V5
   

   Use

   AAV

   Tags

   V5-MBP

   Expression

   Mammalian

   Promoter

   Mouse c-fos promoter

   Available Since

   Oct. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-TRE3G-FNLS-PGK-Puro

   Plasmid

   #110847

   Purpose

   Lentiviral vector for dox-inducible expression of FNLS in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3RA-FNLS
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Mutation

   D10A and NLS sequence at the N-terminus

   Promoter

   TRE3G

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. TRPM7 gRNA (BRDN0001147821)

   Plasmid

   #76112

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TRPM7

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TRPM7 (TRPM7 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG_Xph20-GCaMP7f-CCR5TC

   Plasmid

   #216534

   Purpose

   Encodes a specific PSD-95 binder (Xph20) fused to GCaMP7f, CCR5TC zinc finger, and KRAB(A) transcriptional repressor domain

   Depositor

   Matthieu Sainlos

   Article

   Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

   Insert

   Xph20
   

   Tags

   GCaMP7f

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC19-U6-BsmBI_cassette-Nme/Nme2Cas9-sgRNA (KAC32)

   Plasmid

   #133794

   Purpose

   U6 promoter sgRNA entry vector used for all NmeCas9 or Nme2Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Amrani et al. Genome Biology 2018

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   NmeCas9 and Nme2Cas9 sgRNA entry vector
   

   Expression

   Mammalian

   Mutation

   sgRNA architecture from Amrani et al. Genome Biol…

   Promoter

   U6

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. mP58.dJ1-pCDNA3

   Plasmid

   #21884

   Depositor

   David Ron

   Article

   Oyadomari et al Cell. 2006 Aug 25. 126(4):727-39.

   Insert

   P58 (Dnajc3 Mouse)
   

   Expression

   Mammalian

   Mutation

   DnaJ domain deleted from C-term

   Available Since

   Sept. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSFDuet-v2 RsTAL At4CL

   Plasmid

   #126524

   Purpose

   expresses TAL (Rhodobacter sphaeroides) and 4CL (Arabidopsis thaliana paralog 1) for PYP chromophore biosynthesis

   Depositor

   Andrew Woolley

   Article

   Brechun et al Biochemistry. 2019 Jun 11;58(23):2682-26

   Inserts

   tyrosine ammonia lyase
   p-coumaroyl-CoA ligase (4CL1 Mustard Weed)
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. OCT4-eGFP-2A-Puro

    Plasmid

    #31939

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

    Inserts

    OCT4 (POU5F1 Human)
    eGFP (eGFP )
    

    Use

    Targeting donor

    Tags

    eGFP

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. Integrin beta5-2XEGFP

    Plasmid

    #139779

    Purpose

    Detecting Integrin beta5 by fluorescence microscopy

    Depositor

    Staffan Stromblad

    Article

    Lock et al Nat Cell Biol. 2018 Nov;20(11):1290-1302

    Insert

    Integrin subunit beta5 (ITGB5 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-Ef1a-MCP-huADARE488QT490A

    Plasmid

    #159922

    Purpose

    Fusion of MS2 coat protein to Human ADAR2 E488QT490A, under Ef1a promoter, with WPRE. AKA 116v5

    Depositor

    Fei Chen

    Article

    Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103

    Insert

    Human ADAR2 E488QT490A (ADARB1 Human)
    

    Use

    AAV

    Promoter

    EF1a

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. His-MBP human SPOP (aa 28-337)

    Plasmid

    #52294

    Purpose

    bacterial expression of human SPOP (aa 28-337) fused to His-MBP

    Depositor

    Brenda Schulman

    Article

    Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

    Insert

    SPOP (SPOP Human)
    

    Tags

    His-MBP and TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 11, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pAPM-miR30-IFIH1-ts3

    Plasmid

    #174261

    Purpose

    IFIH1 knockdown

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    IFIH1 shRNA (IFIH1 Human)
    

    Use

    Lentiviral and RNAi

    Promoter

    SFFV

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV_Syn_Xph20_eGFP_CCR5TC

    Plasmid

    #187444

    Purpose

    Encodes a specific PSD-95 binder (Xph20) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

    Insert

    Xph20
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    Synapsin

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. PKD1 gRNA (BRDN0001148399)

    Plasmid

    #76842

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PKD1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PKD1 (PKD1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. PKD1 gRNA (BRDN0001149544)

    Plasmid

    #76841

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PKD1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PKD1 (PKD1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. OCT4-eGFP-PGK-Puro

    Plasmid

    #31937

    Purpose

    Oct4 targeting donor vector

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

    Insert

    OCT4 (POU5F1 Human)
    

    Use

    Targeting donor

    Tags

    eGFP

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. PIK3C2A gRNA (BRDN0001149181)

    Plasmid

    #76414

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIK3C2A

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIK3C2A (PIK3C2A Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. PIK3C2A gRNA (BRDN0001148547)

    Plasmid

    #76415

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIK3C2A

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIK3C2A (PIK3C2A Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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