We narrowed to 10,125 results for: Uty

1. pYI2293

   Plasmid

   #228351

   Purpose

   Constitutive ALX-0081 expression

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

   Insert

   MFalpha(2xAdv)-LE-ALX-0081-3A-FLAG-His
   

   Use

   Affinity Reagent/ Antibody

   Tags

   AAA linker followed by FLAG-His6 and MF_ secretio…

   Expression

   Yeast

   Promoter

   KpGAPDH promoter

   Available Since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. KL094 - pTRE3G-Flag-Halo-CTCF_WT-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor

   Plasmid

   #232930

   Purpose

   Vector to introduce a constitutive rtta3G cassette and a DOX-inducible Flag-Halo-mCTCF WT, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycin selection.

   Depositor

   Danny Reinberg

   Article

   Lucero et al bioRxiv, March 19, 2025

   Insert

   Flag-Halo-CTCF WT
   

   Use

   Mouse Targeting

   Expression

   Mammalian

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pME-MCS (JDW 455)

   Plasmid

   #229832

   Purpose

   A gateway compatible middle entry clone containing an MCS (mutiple cloning site).

   Depositor

   Joshua Wythe

   Article

   Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

   Insert

   attL1/L2 flanked MCS
   

   Use

   Gateway entry clone

   Available Since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSG086

   Plasmid

   #214263

   Purpose

   PaFtsH2H1-link-32-6xHis: PaftsH2(ftsH2_F124-V154del_ins ftsH1_M120-T151)-6xHis cloned in XbaI/NheI site in pET-28a(+)

   Depositor

   Ute Romling

   Article

   Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi

   Insert

   Hybrid PaFtsH protease PaFtsH2H1-link-32 of Pseudomonas aeruginosa SG17M
   

   Tags

   6x His-tag

   Expression

   Bacterial

   Mutation

   substitution of 31 amino acids of FtsH2 (F124RRFA…

   Promoter

   T7 promoter

   Available Since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. L2379-FKBP-GPA(V84R)-20-11S

   Plasmid

   #222901

   Purpose

   Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 11S

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FKBP-GPA(V84R)-20GS-NanoLuc11S
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   V84R in GPA

   Promoter

   CMV

   Available Since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. L2380-FKBP-GPA(V84R)-20-114

   Plasmid

   #222900

   Purpose

   Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 114

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FKBP-GPA(V84R)-20GS-NanoLuc114
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   V84R in GPA

   Promoter

   CMV

   Available Since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. L2378-FRB-GPA(V84R)-20-11S

   Plasmid

   #222899

   Purpose

   Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA(V84R)-20GS-NanoLuc11S
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   V84R in GPA

   Promoter

   CMV

   Available Since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. L2377-FRB-GPA(V84R)-20-114

   Plasmid

   #222898

   Purpose

   Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 114.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA(V84R)-20GS-NanoLuc114
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   V84R in GPA

   Promoter

   CMV

   Available Since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. L2085-WT-GPA-NLuc-Biosensor-Retrovirus-TD135

   Plasmid

   #222875

   Purpose

   Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with split NanoLuciferase reconstitution (11S/114 fragment). WT Glycophorin A (GPA) scaffold.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA(WT)-NanoLuc114-T2A-FKBP-GPA(WT)-NanoLuc11S
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   MSCV LTR

   Available Since

   Aug. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-EF1a-ArgiNLS-miRFP670

    Plasmid

    #220600

    Purpose

    Constitutive expression of a single-cell discriminating version of miRFP670 fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    miRFP670
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-EF1a-ArgiNLS-mVenus-Q69M(ME)

    Plasmid

    #220597

    Purpose

    Constitutive expression of a single-cell discriminating version of mVenus-Q69M (ME) fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    mVenus-Q69M (ME)
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-EF1a-ArgiNLS-mKate2

    Plasmid

    #220599

    Purpose

    Constitutive expression of a single-cell discriminating version of mKate2 fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    mKate2
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. OXGR1-DuET

    Plasmid

    #213363

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    OXGR1 (OXGR1 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. OXER1-DuET

    Plasmid

    #213362

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    OXER1 (OXER1 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. NPY4R-DuET

    Plasmid

    #213355

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    NPY4R (NPY4R Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MRGPRX3-DuET

    Plasmid

    #213346

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    MRGPRX3 (MRGPRX3 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MRGPRE-DuET

    Plasmid

    #213341

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    MRGPRE (MRGPRE Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MRGPRF-DuET

    Plasmid

    #213342

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    MRGPRF (MRGPRF Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HTR1E-DuET

    Plasmid

    #213319

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    HTR1E (HTR1E Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HCA1-DuET

    Plasmid

    #213307

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    HCA1 (HCA1 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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