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We narrowed to 46,595 results for: tra

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Showing: 9101 - 9120 of 46595 results

  1. pAAV-hSyn-DIO {hCAR}off-{DTR-GFP}on-W3SL

    Plasmid
    #111396
    Purpose
    AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON DTR-EGFP (diphtheria toxin receptor for cell ablation), and W3SL cassette (for maximize cloning capacity)
    Depositor
    Adam Kepecs
    Article
    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi
    Inserts
    hCAR (CXADR Human)
    DTR-EGFP
    Use
    AAV
    Tags
    EGFP and Myc
    Expression
    Mammalian
    Promoter
    hSynapsin
    Available Since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    111396-plasmid-map-sequence-id-212262

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  8. pKI_ΩBBMVP16&SRDXWUSm1

    Plasmid
    #220349
    Purpose
    Expresses BBM-VP16 and SRDX-WUSm1 in plant cells
    Depositor
    Tomoko Igawa
    Article
    Sato et al Front Plant Sci. 2024 Apr 3;15:1308417.
    Inserts
    BABY BOOM (BBM Mustard Weed)
    WUSCHEL (WUS Mustard Weed)
    Tags
    Superman Repression Domain X (SRDX) and VP16 tran…
    Expression
    Plant
    Mutation
    two amino acid substitution in WUS-box: L255A L25…
    Promoter
    CaMV 35S and RPS5A
    Available Since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    220349-plasmid-map-sequence-id-440868

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  11. pFS_1113_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23103-Leader-ARRAY2-DR2-FaqI_Term_NotI

    Plasmid
    #184741
    Purpose
    encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23103
    Depositor
    Randall Platt
    Article
    Schmidt et al Science. 2022 May 13;376(6594):eabm6038.
    Insert
    FsRT-Cas1(opt)-Cas2(opt)
    Expression
    Bacterial
    Mutation
    sequence codon optimized for expression in E. coli
    Promoter
    pTetA
    Available Since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    184741-plasmid-map-sequence-id-361527

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  12. pCAG-CBE4max-xCas9(3.7)-P2A-EGFP (RTW4637)

    Plasmid
    #140000
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized CBE4max xCas9(3.7) with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; xCas9(3.7)=A262T/R324L/S409I/E480K/…
    Promoter
    CAG
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140000-plasmid-map-sequence-id-275479

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  13. pBM019

    Plasmid
    #128179
    Purpose
    Construct for generating stable Cas9-expressing Toxoplasma gondii
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2019 Jun 26;4(3). pii: 4/3/e003
    Inserts
    sgRNA#1
    SpCas9
    Chloramphenicol acetyltransferase
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3X FLAG and Nuclear Localization Signal
    Promoter
    TUB1 (Toxoplasma gondii), U6 (Toxoplasma gondii),…
    Available Since
    July 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    128179-plasmid-map-sequence-id-249095

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  14. PMXS-GOT1

    Plasmid
    #72872
    Purpose
    The retroviral GOT1 vector was generated by cloning an sgRNA resistant human GOT1 gene block into the pMXS-ires-blast vector by Gibson Assembly.
    Depositor
    David Sabatini
    Article
    Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10
    Insert
    GOT1 (GOT1 Human)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    sgRNA resistant human GOT1, 7 silent mutations c1…
    Available Since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only

  20. pDmelOR-mRho.V5.mER.hOr56a

    Plasmid
    #126479
    Purpose
    Human codon-optimized D. melanogaster Orco (hOrco) and Or56a (hOr56a). Or56a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cells
    Depositor
    Bill Hansson
    Article
    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.
    Inserts
    Or56a (Or56a Fly)
    Orco (Orco Fly)
    Tags
    H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…
    Expression
    Mammalian
    Mutation
    codon optimization for H. sapiens and codon optim…
    Promoter
    CMV
    Available Since
    June 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    126479-plasmid-map-sequence-id-246521

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Showing: 9101 - 9120 of 46595 results