We narrowed to 59,215 results for: %s

1. p3E-FRB(PLF)-HA no-pA

   Plasmid

   #82597

   Purpose

   3' entry vector containing HA epitope tag fused to C-terminus of FRB mutant (K2095P, T2098L, W2101F) without pA for rapamycin/rapalog induced dimerization

   Depositor

   Kryn Stankunas

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   FRB-HA (no pA)
   

   Use

   Tags

   Expression

   Mutation

   K2095P, T2098L, W2101F

   Promoter

   None

   Available since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-mROCK1-delta5

   Plasmid

   #101293

   Purpose

   Mammalian expression of Rock1-GFP for the study of localisation signaling.

   Depositor

   Alpha Yap

   Article

   Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

   Insert

   Rock1 (Rock1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   375-727 aa

   Promoter

   CMV

   Available since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmCellFree_KA2_ZIKV NS2A

   Plasmid

   #145363

   Purpose

   Cell-free/mammalian expression vector for Zika virus NS2A protein with N-term mCherry

   Depositor

   Rob Parton

   Article

   Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

   Insert

   ZIKV NS2A
   

   Use

   Tags

   His and mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   T7- CMV/CAG

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBT296_pBS-U-attP-FRT5-pSV40-Neo-pA-FRT5

   Plasmid

   #52537

   Purpose

   Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

   Depositor

   Liqun Luo

   Article

   Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

   Inserts

   PhiC31o attP (full length)
   Neo
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBT298_pBS-U-attPx3-FRT5-pSV40-Neo-pA-FRT5

   Plasmid

   #52538

   Purpose

   Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

   Depositor

   Liqun Luo

   Article

   Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

   Inserts

   PhiC31o attPx3 (70 bp each)
   Neo
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MDH1-PGK-FLAG-TAT-86-A

   Plasmid

   #35977

   Depositor

   Susana Valente

   Article

   Mediouni et al Curr HIV Res. 2015;13(1):64-79.

   Insert

   transactivating protein (tat Human Immunodeficiency Virus)
   

   Use

   RNAi and Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   From the "A" HIV clade

   Promoter

   Available since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSB165 - pL1R3_pMAS::rLUC-I::tMAS

   Plasmid

   #123198

   Purpose

   binary plant vector for transient expression of a Renilla luciferase (rLUC, with intron)

   Depositor

   Erin Cram, Carolyn Lee-Parsons

   Article

   Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

   Insert

   pMAS::rLUC-I::tMAS
   

   Use

   Luciferase

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAc5.1B-EGFP-DmCup_C

   Plasmid

   #146041

   Purpose

   Insect Expression of DmCup

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmCup (cup Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   P418S

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAc5.1B-EGFP-DmLSm4_A

   Plasmid

   #145883

   Purpose

   Insect Expression of DmLSm4

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

   Insert

   DmLSm4 (LSm-4 Fly)
   

   Use

   Tags

   V5-6xHis

   Expression

   Insect

   Mutation

   two silent mutations compared to the sequence of …

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. BPK1160

    Plasmid

    #179298

    Purpose

    Expresses dCas9 fused to P65

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

    Insert

    dCas9-p65
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive Cas9)

    Promoter

    CAG

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFX-GalT-Venus

    Plasmid

    #174472

    Purpose

    Express Venus with golgi apparatus-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    Venus with golgi apparatus-targeting sequence
    

    Use

    Tags

    β1,4-galactose transferase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFX-Tom20-Venus

    Plasmid

    #174187

    Purpose

    Express Venus with mitochondria-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    Venus with mitchondria-targeting sequence
    

    Use

    Tags

    the mitochondria-targeting sequence of TOM20

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSW179 - pML1

    Plasmid

    #115977

    Purpose

    entry vector for GreenGate cloning method, promoter

    Depositor

    Jan Lohmann

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pML1
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBacPAK8-His-myc-Uba1

    Plasmid

    #29508

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

    Insert

    Uba1 (UBA1 Budding Yeast)
    

    Use

    Tags

    His and myc

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pRSET FLIPglu-600uDelta11 Ares-Aphrodite

    Plasmid

    #13571

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

    Insert

    FLIPglu F16A Delta11 Aphrodite
    

    Use

    Tags

    Aphrodite and Ares

    Expression

    Bacterial

    Mutation

    Fluorophore-derived linker shortened. F16A mutati…

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUHD Cdk2 DN HA

    Plasmid

    #27651

    Depositor

    Greg Enders

    Article

    Hu et al Mol Cell Biol. 2001 Apr . 21(8):2755-66.

    Insert

    Cdk2 (CDK2 Human)
    

    Use

    Tags

    HA (YDVPDYA)

    Expression

    Mammalian

    Mutation

    D146N (Dominant Negative)

    Promoter

    Available since

    Feb. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pCMV-ABE

    Plasmid

    #107723

    Purpose

    For expression of ABE7.10 in mammalian cells

    Depositor

    Xingxu Huang

    Article

    Liu et al Nat Commun. 2018 Jun 14;9(1):2338. doi:

    Insert

    ABE7.10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET22b-ecDHFR-G51PEKN

    Plasmid

    #118582

    Purpose

    Bacterial expression of E.coli DHFR G51PEKN mutant

    Depositor

    Stephen Benkovic

    Article

    Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

    Insert

    DHFR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G51 mutated to P51; Glu-Lys-Asn residues inserted…

    Promoter

    T7

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-Zif268

    Plasmid

    #69224

    Purpose

    Expresses R1335K mutant SpCas9 fused to Zif268 in mammalian cell

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS and Zif268

    Expression

    Mammalian

    Mutation

    Arginine 1335 is changed to Lysine

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pmCellFree _KA2_ZIKV NS5

    Plasmid

    #145368

    Purpose

    Cell-free/mammalian expression vector for Zika virus NS5 protein with N-term mCherry

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Insert

    ZIKV NS5
    

    Use

    Tags

    8xHis and mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    T7- CMV/CAG

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge