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pcDNA3.1/NDUFS2 T58A/myc-His

Plasmid

#127498

Purpose

Expresses myc-tagged NDUFS2/T58A in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 T58A (NDUFS2 Human)

Tags

myc-His

Expression

Mammalian

Mutation

NDUFS2 T58A

Promoter

CMV

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS2 H60A/myc-His

Plasmid

#127499

Purpose

Expresses myc-tagged NDUFS2/H60A in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 H60A (NDUFS2 Human)

Tags

myc-His

Expression

Mammalian

Mutation

NDUFS2 H60A

Promoter

CMV

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS2 W61A/myc-His

Plasmid

#127500

Purpose

Expresses myc-tagged NDUFS2/W61A in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 W61A (NDUFS2 Human)

Tags

myc-His

Expression

Mammalian

Mutation

NDUFS2 W61A

Promoter

CMV

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS2 K62A/myc-His

Plasmid

#127501

Purpose

Expresses myc-tagged NDUFS2/K62A in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 K62A (NDUFS2 Human)

Tags

myc-His

Expression

Mammalian

Mutation

NDUFS2 K62A

Promoter

CMV

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS2 P63A/myc-His

Plasmid

#127502

Purpose

Expresses myc-tagged NDUFS2/P63A in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 P63A (NDUFS2 Human)

Tags

myc-His

Expression

Mammalian

Mutation

NDUFS2 P63A

Promoter

CMV

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pJC20cdc8.A18T

Plasmid

#99360

Purpose

Bacterial expression vector containing cDNA encoding for the fission yeast tropomyosin, Cdc8 with the amino acid substitution Ala-18-Thr.

Depositor

Dan Mulvihill

Article

Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

Insert

cdc8 (cdc8 Fission Yeast)

Expression

Bacterial

Mutation

Alanine 18 to Threonine

Promoter

T7

Available Since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pJC20cdc8.E31K

Plasmid

#99361

Purpose

Bacterial expression vector containing cDNA encoding for the fission yeast tropomyosin, Cdc8 with the amino acid substitution Glu-31-Lys.

Depositor

Dan Mulvihill

Article

Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

Insert

cdc8 (cdc8 Fission Yeast)

Expression

Bacterial

Mutation

Glutamic acid 31 to Lysine

Promoter

T7

Available Since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pAc-GFP-dSUMOdeltaC

Plasmid

#85697

Purpose

expression of GFP-SUMO1 delta C in Drosophila cells

Depositor

Licio Collavin, Giannino Del Sal

Article

Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

Insert

SUMO (Sumo Fly)

Tags

GFP

Expression

Insect

Mutation

non-conjugatable SUMO, lacking the C-terminal gly…

Promoter

pAc5

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only
pMAL-PIAL2M-sim1

Plasmid

#67908

Purpose

Inducible expression of a mutant of PIAL2M where the SIM interacting motif 1 (VFDL 425-428) was substituted with alanines.

Depositor

Andreas Bachmair

Article

Tomanov et al Plant Cell. 2014 Nov;26(11):4547-60. doi

Insert

PIAL2 (AT5G41580 Mustard Weed)

Tags

MBP

Expression

Bacterial

Mutation

E281-A496; VEDL 425-428 AAA

Promoter

P-lac

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only
Kv7.4 AD (325-645, C643A, Δ 368-492)/I346A/pET28HMT

Plasmid

#111398

Purpose

Protein expression in E.Coli

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Tags

His Tag and MBP Tag

Expression

Bacterial

Mutation

Kv7.4 AD (325-645, C643A, Δ 368-492)/I346A

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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Kv7.4 AD (325-645, C643A, Δ 368-492)/I346D/pET28HMT

Plasmid

#111399

Purpose

Protein expression in E.Coli

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Tags

His Tag and MBP Tag

Expression

Bacterial

Mutation

Kv7.4 AD (325-645, C643A, Δ 368-492)/I346D

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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Kv7.4 AD (325-645, C643A, Δ 368-492)/S536A/L536A/pET28HMT

Plasmid

#111400

Purpose

Protein expression in E.Coli

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Tags

His Tag and MBP tag

Expression

Bacterial

Mutation

Kv7.4 AD (325-645, C643A, Δ 368-492)/S536A/L540A

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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Kv7.4 AD (325-645, C643A, Δ 368-492)/S536D/L540D/pET28HMT

Plasmid

#111451

Purpose

Protein expression in E.Coli

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Tags

His Tag and MBP tag

Expression

Bacterial

Mutation

Kv7.4 AD (325-645, C643A, Δ 368-492)/S536D/L540D

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC6A8_STOP

Plasmid

#161081

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC6A8 (SLC6A8 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_OCA2

Plasmid

#132253

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

OCA2 (OCA2 Human)

Expression

Mammalian

Available Since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC3A2

Plasmid

#132213

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC3A2 (SLC3A2 Human)

Expression

Mammalian

Available Since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC7A5

Plasmid

#132197

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC7A5 (SLC7A5 Human)

Expression

Mammalian

Available Since

Oct. 28, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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TERT - WT

Plasmid

#213827

Purpose

Expresses human telomerase reverse transcriptase, the catalytic subunit of telomerase

Depositor

Coleman Lindsley

Article

Reilly et al Blood. 2021 Sep 9;138(10):898-911. doi:

Insert

human TERT (TERT Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Tet-responsive promoter PTight, consisting of sev…

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMDC32B-AtTAS1c-D2-B/c-AtMIR173

Plasmid

#137885

Purpose

Plant expression vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor downstream 3'D1[+]. Contains AtMIR173 for syntasi expression in any plant species.

Depositor

Alberto Carbonell

Article

Lopez-Dolz et al Nucleic Acids Res. 2020 May 12. pii: 583

Inserts

AtTAS1c-D2-B/c
2x35S-AtMIR173-Tnos

Expression

Plant

Mutation

A. thaliana TAS1c precursor sequence including a …

Available Since

May 14, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC35F2_STOP

Plasmid

#161287

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35F2 (SLC35F2 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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