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pET28a MISTIC

Plasmid

#85999

Purpose

bacterial expression of transmembrane proteins using N-terminal MISTIC and C-terminal HIS6 tag

Depositor

Wolfram Antonin

Publication

Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

Insert

N/A

Tags

HIS6 and MISTIC

Expression

Bacterial

Mutation

codon optimzed for bacterial expression

Promoter

T7

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pRZ-CAS9-mCherry

Plasmid

#80974

Purpose

Cas9, mCherry expression

Depositor

Veit Hornung

Publication

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

Cas9

Expression

Mammalian

Promoter

CMV

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCIRCgp41-1

Plasmid

#74227

Purpose

Vector for SICLOPPS-mediated protein circularization using the gp41-1 intein.

Depositor

Barbara Di Ventura, Roland Eils

Publication

Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

Type

Empty backbone

Use

Synthetic Biology

Tags

gp41-1 C-intein and gp41-1 N-intein

Expression

Bacterial

Promoter

T7-lac

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTM1_VP30_ZEBOV

Plasmid

#69119

Purpose

Encodes for Ebola Virus Zaire VP30 under control of a T7 RNA polymerase promoter

Depositor

Elke Mühlberger

Publication

Trunschke et al Virology. 2013 Jul 5;441(2):135-45. doi:

Insert

VP30 ZEBOV (VP30 )

Use

T7

Promoter

T7

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pFF745

Plasmid

#61450

Purpose

IPTG-inducible SCRIBE_kanR(ON)

Depositor

Timothy Lu

Publication

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

SCRIBE_kanR(ON)

Use

Synthetic Biology

Promoter

pLacO

Available since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pACλCI

Plasmid

#53730

Purpose

PlacUV5-directed synthesis of the λCI protein; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53733.

Depositor

Ann Hochschild

Publication

Dove et al Nature. 1997 Apr 10;386(6625):627-30.

Insert

bacteriophage lambda CI protein

Expression

Bacterial

Promoter

lac UV5

Available since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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plenti.CamKII.(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3

Plasmid

#209725

Purpose

Expresses cytosolic low affinity ratiometric ATP sensor

Depositor

Timothy Ryan

Publication

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3

Use

Lentiviral

Expression

Mammalian

Promoter

CamKII

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-mNeon-PuroR

Plasmid

#174090

Purpose

CRISPaint gene tagging

Depositor

Veit Hornung

Publication

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

mNeonGreen

Use

Gene tagging

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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AbVec2.0-mIghg1

Plasmid

#127158

Purpose

Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG1 isotype

Depositor

Hedda Wardemann

Insert

Ighg1 (Ighg1 Mouse)

Expression

Mammalian

Promoter

hCMV IE1 gene promoter

Available since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-LumiScarlet

Plasmid

#126623

Purpose

A BRET-based mScarlet-I-LumiLuc fusion for red-shifted bioluminescence emission

Depositor

Huiwang Ai

Publication

Yeh et al ACS Chem Biol. 2019 Apr 17. doi: 10.1021

Insert

LumiScarlet

Expression

Mammalian

Promoter

CMV

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pSCAC-69

Plasmid

#31241

Purpose

Mitochondrial localization of EGFP, for real-time imaging of mitochondria in transgenic zebrafish

Depositor

Seok-Yong Choi

Publication

Kim et al Biotechniques. 2008 Sep . 45(3):331-4.

Insert

mitochondrial localization enhanced green fluorescent protein

Available since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMB1609_pRR-EGFP

Plasmid

#65852

Purpose

EGFP-based homologous recombination-dependent reporter for genome editing with TALENs or CRISPR/Cas9

Depositor

Marc Bühler

Publication

Flemr et al Cell Rep. 2015 Jul 28;12(4):709-16. doi:

Insert

split EGFP coding sequence

Use

CRISPR and TALEN

Expression

Mammalian

Promoter

SV40

Available since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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HO-pGAL-poly-KanMX4-HO

Plasmid

#51664

Purpose

yeast plasmid for integration of target sequence at HO locus, Kan selection, pGAL inducible promoter

Depositor

David Stillman

Publication

Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

Type

Empty backbone

Use

Yeast integration

Expression

Yeast

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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MSCV-Six1

Plasmid

#49263

Purpose

retroviral expression of human Six1 and GFP

Depositor

Heide Ford

Insert

Six1 (SIX1 Human)

Use

Retroviral

Tags

IRES-GFP

Expression

Mammalian

Available since

Nov. 5, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGEXndoS

Plasmid

#44655

Depositor

Mattias Collin

Publication

Collin et al Infect Immun. 2001 Nov . 69(11):7187-9.

Insert

ndoS (ndoS Streptococcus pyogenes)

Tags

Gst

Expression

Bacterial

Mutation

None

Promoter

tac

Available since

May 20, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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C5A

Plasmid

#27799

Depositor

Steven Vogel

Publication

Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

Insert

Cerulean-5-Amber

Use

Fret donor control

Tags

Amber and Cerulean

Expression

Mammalian

Available since

May 23, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pEB1029

Plasmid

#22066

Depositor

Emmanuelle Bouveret

Publication

Battesti et al Proteomics. 2008 Nov . 8(22):4768-71.

Type

Empty backbone

Tags

T25-Flag

Expression

Bacterial

Available since

Oct. 22, 2009

Availability

Academic Institutions and Nonprofits only

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pSAC5.02

Plasmid

#16077

Depositor

Jochen Buck, Lonny Levin

Publication

Buck et al Proc Natl Acad Sci U S A. 1999 Jan 5. 96

Insert

soluble adenylyl cyclase (Adcy10 Rat)

Expression

Mammalian

Available since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pMM135

Plasmid

#127216

Purpose

BeYDV replicon with constitutive Luc expression, WUS2, sgRNA targeting PDS

Depositor

Daniel Voytas

Publication

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, WUS2, sgRNA

Expression

Plant

Mutation

codon optimized CDSs

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-GDH

Plasmid

#108954

Purpose

heterologous expression of GDH of B. megaterium in E. coli

Depositor

Frank Schulz

Publication

Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

Insert

Glucose dehydrogenase IV (GDH IV)

Tags

His6-Tag

Expression

Bacterial

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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