We narrowed to 4,753 results for: TIL

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pCMV-P-RAN-RFP6

Plasmid

#106411

Purpose

P-RAN-RFP6 (RANbody RFP6::vHRP)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

P-RAN-RFP6

Tags

HA and His

Expression

Mammalian

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pTK295

Plasmid

#59564

Purpose

SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak EGFP

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(14) 2xK-turn Kozak EGFP

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-ALFA

Plasmid

#198301

Purpose

Produces PVCs retargeted with ALFA tag

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-ALFA

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pX330A_dCas9-1x2

Plasmid

#63596

Purpose

Expresses dCas9 and gRNA

Depositor

Takashi Yamamoto

Article

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Insert

humanized S. pyogenes dCas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D1361Y

Promoter

CBh

Available Since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR/pSM2(CMV) (w57-1)

Plasmid

#17389

Purpose

Gateway entry vector for miRNA expression under CMV promoter

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning and RNAi

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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SmoCFP

Plasmid

#128156

Purpose

Expression of Smoothened

Depositor

Jennifer Lippincott-Schwartz

Article

Ott et al Cilia. 2012 Apr 25;1(1):3. doi: 10.1186/

Insert

Smoothened (Smo Mouse)

Expression

Mammalian

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-13Myc-TRP1

Plasmid

#41601

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

TRP1 (TRP1 Budding Yeast)

Use

Yeast genomic targeting

Tags

13Myc and yeast ADH1 terminator

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pFA6a-GFP(S65T)-TRP1

Plasmid

#41597

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

TRP1 (TRP1 Budding Yeast)

Use

Yeast genomic targeting

Tags

GFP (S65T) and yeast ADH1 terminator

Available Since

Jan. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-Golden-Gate-Esp3I-Destination

Plasmid

#51278

Purpose

CAG promoter-driven expression vector suitable for ligation of multiple inserts by Golden Gate Cloning using Esp3I

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Type

Empty backbone

Expression

Mammalian

Promoter

CAG

Available Since

March 4, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR1A-FKBP12DD C-term (w350-2)

Plasmid

#17413

Purpose

Gateway entry vector for Degron, C-term

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning

Tags

FKBP12DD (L106P)

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pCAG-b3STOPb3-ZsGreen

Plasmid

#51266

Purpose

B3-responsive fluorescent reporter plasmid containing a STOP-cassette flanked by B3-recognition sites followed by ZsGreen CDS

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

b3STOPb3-ZsGreen

Expression

Mammalian

Promoter

CAG

Available Since

March 4, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR1A-NTAP-A (w322-1)

Plasmid

#17402

Purpose

Gateway entry vector for NTAP (N-terminal affinity purification), frame A

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning

Tags

CBP and SBP

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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p206_LTJ_sgRNACD45.2_R3

Plasmid

#82674

Purpose

sgRNA targeting murine CD45.2 region 3. Co-expresses SpCas9-2A-EGFP.

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

sgRNA targeting CD45.2 region 3

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CamKII-DIO-mCherry

Plasmid

#190241

Purpose

Expresses mCherry flanked by DIO

Depositor

Young J Yoon

Article

Nasrallah et al Proc Natl Acad Sci U S A. 2022 Aug 9;119

Insert

mCherry

Use

AAV

Tags

N/A

Promoter

mouse CamKII alpha

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pNIR_JNKbiosensor

Plasmid

#127440

Purpose

Mammalian expression of the NIR biosensor for detecting JNK activity

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

NIR JNK biosensor

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pFBNb-cALFA

Plasmid

#159990

Purpose

For the protein expression with ALFA tag at C-terminus in insect cells

Depositor

Motoyuki Hattori

Article

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Type

Empty backbone

Tags

HRV3C protease cleavage site-ALFA tag-8histidine

Expression

Insect

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-A4DARPin

Plasmid

#198288

Purpose

Produces PVCs retargeted with anti-lysozyme DARPin A4

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-A4DARPin

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pFA6a-hphMX6-81nmt1

Plasmid

#105164

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Ye et al Mol Biol Cell. 2012 Apr;23(7):1181-95. d

Insert

hphMX6-81nmt1

Tags

81nmt1

Expression

Bacterial and Yeast

Promoter

81nmt1

Available Since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-hphMX6-41nmt1

Plasmid

#105163

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Ye et al Mol Biol Cell. 2012 Apr;23(7):1181-95. d

Insert

hphMX6-41nmt1

Tags

41nmt1

Expression

Bacterial and Yeast

Promoter

41nmt1

Available Since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-P-RAN-GFP41

Plasmid

#106407

Purpose

P-RAN-GFP41 (RANbody GFP41::vHRP)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

P-RAN-GFP41

Tags

HA and His

Expression

Mammalian

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-hphMX6-3nmt1

Plasmid

#105162

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Ye et al Mol Biol Cell. 2012 Apr;23(7):1181-95. d

Insert

hphMX6-3nmt1

Tags

3nmt1

Expression

Bacterial and Yeast

Promoter

3nmt1

Available Since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Y-RAN-GFP26

Plasmid

#106423

Purpose

Y-RAN-GFP26 (RANbody GFP26::Chicken IgY)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

Y-RAN-GFP26

Tags

HA and His

Expression

Mammalian

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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5HT2A-APEX2

Plasmid

#215558

Purpose

Stable, inducible expression of 5HT2A

Depositor

Nevan Krogan

Article

Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

Insert

Flag tag-5HT2A(1-452)-APEX2-5HT2A (453-471)

Use

Lentiviral

Available Since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pQCXP CMV/TO LUC (w432-1)

Plasmid

#17476

Purpose

Retroviral Luciferase expression vector, CMV/TO promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly Luciferase

Use

Luciferase and Retroviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pJL2-mGold-P2A-EBFP2

Plasmid

#157998

Purpose

Co-expression of mGold and EBFP2 in mammalian cells.

Depositor

Francois St-Pierre

Article

Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

Insert

mGold-P2A-EBFP2

Expression

Mammalian

Mutation

mGold is mVenus with L46F;T63S mutations

Promoter

CMV

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pMulti-sgRNA-LacZ-DsRed

Plasmid

#99914

Purpose

Receptor plasmid for the assembly of multiple sgRNAs with DsRed coexpression

Depositor

Yujie Sun

Article

Shao et al ACS Synth Biol. 2017 Sep 7. doi: 10.1021

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pHCKan-yibDp-ald*-alaE

Plasmid

#134939

Purpose

Low phosphate inducible alanine dehydrogenase and alanine exporter

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

alanine dehydrogenase and L-alanine exporter

Expression

Bacterial

Promoter

yibD

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pJ211_WT_low

Plasmid

#128848

Purpose

Low copy number plasmid for expression of E. coli flagellin from its natural promoter

Depositor

Mark Pallen

Article

Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

Insert

Flagellin

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pLVU-tTR-KRAB

Plasmid

#11645

Purpose

Tet-regulated (Tet-on) lentiviral vector for transgene (hUbiquitin promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 3rd generation

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

hUbiquitin C, GFP, tTR-KRAB, no tetO

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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VKG1-gRNA-pX330

Plasmid

#108671

Purpose

Cleavage of VKG1 sequence by CRISPR/Cas9

Depositor

Shigeo Sugano

Article

Sawatsubashi et al Sci Rep. 2018 Jan 12;8(1):593. doi: 10.1

Insert

gRNA for VKG1 sequence

Use

CRISPR

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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