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Plasmid#174444Purposein vitro transcription template for mouse CENP-C (N-ter EGFP tagged)DepositorInsertCentromere protein C
UseIn vitro transcription templateTagsEGFPExpressionMutationPromoterT7 SP6Available sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV mTurquoise2-superYFP tandem
Plasmid#157776PurposeProduces a fusion between mTurquoise2 and superYFP. It can be used as a positive control for FRET (using FLIM)DepositorTypeEmpty backboneUseTagsmTurquoise2 and superYFPExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Zfp36-3'UTR
Plasmid#61780PurposeLuciferase reporter containing the 3'UTR of mouse Zfp36DepositorInsertmouse Zfp36 3'UTR
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pExodus
Plasmid#39991DepositorTypeEmpty backboneUseTagsExpressionBacterial and MammalianMutationPromoterCMV, T7Available sinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCVL.SFFV.Kozak.HA.NLS.Y2Ani.IRES.BFP
Plasmid#45578PurposeExpresses Y2 variant of I-Ani I nuclease in mammalian cells with BFP trackerDepositorInsertcoY2 Ani
UseLentiviralTagsHA, IRES BFP, and NLSExpressionMammalianMutationco indicates codon optimization for mammalian exp…PromoterSFFVAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) neo with Smad5 CDS
Plasmid#61800PurposepcDNA3.1(+) with full length mouse Smad5 CDSDepositorInsertSmad5 CDS (Smad5 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pGFPmAID_htz-1
Plasmid#174214PurposeDonor plasmid for tagging htz-1 with GFP::mAIDDepositorInsertGFP::mAID::htz-1 tagging donor
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-DKAR-Nuc
Plasmid#45499DepositorInsertDKAR-Nuc
UseTagsCFP, NLS, and YFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
GFP-PRMT9 L182A/D183A/I184A/G185A
Plasmid#79676Purposemammalian expression of GFP-PRMT9 catalytically inactive LDIG(182-185)AAAA mutantDepositorInsertPRMT9 (PRMT9 Human)
UseTagsEGFPExpressionMammalianMutationL182A/D183A/I184A/G185A catalytic mutantPromoterCMVAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV mTurquoise2-shadowY tandem
Plasmid#157775PurposeProduces a fusion between mTurquoise2 and shadowY. It can be used as a positive control for FRET (using FLIM)DepositorTypeEmpty backboneUseTagsmTurquoise2 and shadowYExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMM0647
Plasmid#129008PurposeIntegration vector for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 markerDepositorInsertKanR-ColE1 URA 5'-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1--pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
GST-PRMT9 L182A/D183A/I184A/G185A
Plasmid#67608Purposebacterial expression of catalytically inactive GST-PRMT9 LDIG(182-185)AAAA mutantDepositorInsertPRMT9 (PRMT9 Human)
UseTagsGSTExpressionBacterialMutationcatalytic mutant LDIG(182-185)AAAAPromotertacAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtT5R1AtR2NtB2
Plasmid#160888PurposeExpression of N. tabacum Rubisco small subunit (rbcS-T5), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-T5
UseTagsExpressionBacterialMutationPromoterrbcS-T5: T7, chaperones: T11Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtT1R1AtR2NtB2
Plasmid#160885PurposeExpression of N. tabacum Rubisco small subunit (rbcS-T1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-T1
UseTagsExpressionBacterialMutationPromoterrbcS-T1: T7, chaperones: T8Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtS5R1AtR2NtB2
Plasmid#160884PurposeExpression of N. tabacum Rubisco small subunit (rbcS-S5), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-S5
UseTagsExpressionBacterialMutationPromoterrbcS-S5: T7, chaperones: T7Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtS2R1AtR2NtB2
Plasmid#160883PurposeExpression of N. tabacum Rubisco small subunit (rbcS-S2), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertrbcS-S2, rbcX, raf1, raf2, bsd2
UseTagsExpressionBacterialMutationPromoterrbcS-S2: T7, chaperones: T7Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1T1
Plasmid#97327PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional T residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1T1
UseTags6xHis - TEVExpressionBacterialMutationAddition of T residue after T25PromoterT7Available sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1T2
Plasmid#97328PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include two additional T residues shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1T2
UseTags6xHis - TEVExpressionBacterialMutationAddition of two T residues after T25PromoterT7Available sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGCaMP5
Plasmid#78705PurposeGCaMP5 under the Toxoplasma SAG1 5' UTR. No Toxoplasma selectable markerDepositorInsertGCaMP5
UseTagsExpressionMutationPromoterSAG1 5' UTRAvailable sinceMay 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Snail1-3'UTR
Plasmid#61781PurposeLuciferase reporter containing the 3'UTR of mouse Snail1DepositorInsertmouse Snail1 3'UTR
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCVL TAL(Nd154C63) SFFV Age-Xho/Xba-Sal linkers
Plasmid#50421PurposepTAL plasmid for cloning of megaTALs or other TAL-fusions and expression in mammalian cells. RVDs are cloned between Age-Xho sites and meganuclease/fusion is cloned between Xba-Sal sites.DepositorTypeEmpty backboneUseLentiviral and TALENTagsExpressionMammalianMutationPromoterSFFVAvailable sinceMay 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCVL TAL(Nd154C63) SFFV Xba-Sal linker
Plasmid#50420PurposepTAL plasmid compatible with the Golden Gate TALEN cloning system. For cloning of megaTALs or other TAL-fusions and expression in mammalian cells. Meganuclease/fusion is cloned between Xba-Sal sites.DepositorTypeEmpty backboneUseLentiviral and TALENTagsExpressionMammalianMutationPromoterSFFVAvailable sinceMay 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Ywhag-3'UTR
Plasmid#61778PurposeLuciferase reporter containing the 3'UTR of mouse YwhagDepositorInsertmouse Ywhag 3'UTR
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Xbp1-3'UTR
Plasmid#61779PurposeLuciferase reporter containing the 3'UTR of mouse Xbp1DepositorInsertmouse Xbp1 3'UTR
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pACYC-yhaM-RFP
Plasmid#207651PurposeOverexpression of native L-cysteine desulfidase and RFP in E. coli MG1655DepositorInsertyhaM
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSmart-EF1alpha-NlaCas7-p65
Plasmid#207394PurposeExpresses NlaCas7-p65 activator fusion in mammalian cellsDepositorInsertEF1a-NlaCas7-p65-bGH polyA
UseTagsHA and NLSExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMuLE (R4-R3) PRB
Plasmid#206164PurposepMuLE R4-R3 entry plasmid containing human full length PRB gene, for 3-component Gateway assemblyDepositorInsertProgesteron receptor B (PGR Human)
UseGateway cloning vectorTagsExpressionMutationPromoter-Available sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
MT2A_beta-domain
Plasmid#200300PurposeBacterial expression plasmids for production of recombinant beta-domain of metallothionein 2aDepositorInsertbeta-domain of metallothionein 2a (MT2A Human)
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationPromoterT7Available sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only