Skip to main content
Addgene
Showing: 1351 - 1380 of 3090 results
  1. pMM0647

    Plasmid
    #129008
    Purpose
    Integration vector for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 marker
    Depositor
    Insert
    KanR-ColE1 URA 5'-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1--pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
  2. pCDF-NtXNtT5R1AtR2NtB2

    Plasmid
    #160888
    Purpose
    Expression of N. tabacum Rubisco small subunit (rbcS-T5), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.
    Depositor
    Insert
    PT7-Nt-rbcS-T5
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    rbcS-T5: T7, chaperones: T11
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
  3. pCDF-NtXNtT1R1AtR2NtB2

    Plasmid
    #160885
    Purpose
    Expression of N. tabacum Rubisco small subunit (rbcS-T1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.
    Depositor
    Insert
    PT7-Nt-rbcS-T1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    rbcS-T1: T7, chaperones: T8
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
  4. pCDF-NtXNtS5R1AtR2NtB2

    Plasmid
    #160884
    Purpose
    Expression of N. tabacum Rubisco small subunit (rbcS-S5), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.
    Depositor
    Insert
    PT7-Nt-rbcS-S5
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    rbcS-S5: T7, chaperones: T7
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
  5. pCDF-NtXNtS2R1AtR2NtB2

    Plasmid
    #160883
    Purpose
    Expression of N. tabacum Rubisco small subunit (rbcS-S2), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.
    Depositor
    Insert
    rbcS-S2, rbcX, raf1, raf2, bsd2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    rbcS-S2: T7, chaperones: T7
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
  6. pMSP1D1T1

    Plasmid
    #97327
    Purpose
    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional T residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.
    Depositor
    Insert
    MSP1D1T1
    Use
    Tags
    6xHis - TEV
    Expression
    Bacterial
    Mutation
    Addition of T residue after T25
    Promoter
    T7
    Available since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
  7. pMSP1D1T2

    Plasmid
    #97328
    Purpose
    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include two additional T residues shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.
    Depositor
    Insert
    MSP1D1T2
    Use
    Tags
    6xHis - TEV
    Expression
    Bacterial
    Mutation
    Addition of two T residues after T25
    Promoter
    T7
    Available since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
  8. pCVL TAL(Nd154C63) SFFV Age-Xho/Xba-Sal linkers

    Plasmid
    #50421
    Purpose
    pTAL plasmid for cloning of megaTALs or other TAL-fusions and expression in mammalian cells. RVDs are cloned between Age-Xho sites and meganuclease/fusion is cloned between Xba-Sal sites.
    Depositor
    Type
    Empty backbone
    Use
    Lentiviral and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV
    Available since
    May 23, 2014
    Availability
    Academic Institutions and Nonprofits only
  9. pCVL TAL(Nd154C63) SFFV Xba-Sal linker

    Plasmid
    #50420
    Purpose
    pTAL plasmid compatible with the Golden Gate TALEN cloning system. For cloning of megaTALs or other TAL-fusions and expression in mammalian cells. Meganuclease/fusion is cloned between Xba-Sal sites.
    Depositor
    Type
    Empty backbone
    Use
    Lentiviral and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV
    Available since
    May 23, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 1351 - 1380 of 3090 results