We narrowed to 8,996 results for: App;

1. pJK538

   Plasmid

   #72445

   Purpose

   Produces Acetobacter aceti 1023 small hypothetical protein with N-terminal His6 tag (H6AaOrfX); N5-carboxyaminoimidazole ribonucleotide mutase (AaPurE1)

   Depositor

   T. J. Kappock

   Inserts

   hypothetical protein
   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7 and n/a

   Available since

   Feb. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJK640

   Plasmid

   #72467

   Purpose

   Encodes Escherichia coli evgA

   Depositor

   T. J. Kappock

   Insert

   response regulator in two-component regulatory system with EvgS (evgA Escherichia coli str. K-12 substr. MG1655)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJK641

   Plasmid

   #72468

   Purpose

   Encodes Escherichia coli evgA (Plac noncoding)

   Depositor

   T. J. Kappock

   Insert

   response regulator in two-component regulatory system with EvgS (evgA Escherichia coli str. K-12 substr. MG1655)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJK646

   Plasmid

   #72469

   Purpose

   Encodes Escherichia coli evgA (Plac coding)

   Depositor

   T. J. Kappock

   Insert

   response regulator in two-component regulatory system with EvgS (evgA Escherichia coli str. K-12 substr. MG1655)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   none

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJK558

   Plasmid

   #71704

   Purpose

   Produces Acetobacter aceti 1023 thioredoxin reductase 1 with Cys138>Ser mutant (AaTrxB1-C138S)

   Depositor

   T. J. Kappock

   Insert

   thioredoxin reductase 1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes cysteine-138 to serine

   Promoter

   T7

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJK581

   Plasmid

   #71695

   Purpose

   Produces Acetobacter aceti 1023 thioredoxin with a C-terminal His6 tag and Cys35>Ser mutant (AaTrxAH6-C35S)

   Depositor

   T. J. Kappock

   Insert

   thioredoxin
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changes cysteine-35 to serine

   Promoter

   T7

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJK505

   Plasmid

   #71594

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe mutant (AaPurE1fww)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes tryptophan-34 to phenylalanine, additiona…

   Promoter

   T7

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJK506

   Plasmid

   #71596

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp165>Phe mutant (AaPurE1wwf)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes tryptophan-165 to phenylalanine, addition…

   Promoter

   T7

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDC10

   Plasmid

   #75077

   Purpose

   Produces Escherichia coli glycinamide ribonucleotide synthetase (H6EcPurD)

   Depositor

   T. J. Kappock, JoAnne Stubbe

   Insert

   phosphoribosylglycinamide synthetase (purD Escherichia coli)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   adds unique SacII site at Thr95-Ala96 by a silent…

   Promoter

   T7

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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10. pK19pA-MN

    Plasmid

    #86973

    Purpose

    ChIC; genomic mapping of chromatin proteins

    Depositor

    Ulrich Laemmli

    Article

    Schmid et al Mol Cell. 2004 Oct 8;16(1):147-57.

    Insert

    protein_A fused to Staphylococcus aureus microccocal nuclease (MNase)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJK131

    Plasmid

    #71548

    Purpose

    Produces Geobacillus stearothermophilus (Bacillus stearothermophilus) alanine racemase (alr)

    Depositor

    T. J. Kappock

    Article

    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

    Insert

    Alanine racemase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. N1-moxDendra2

    Plasmid

    #89792

    Purpose

    C-terminal fusion to moxDendra2

    Depositor

    Erik Snapp

    Article

    Kaberniuk et al Chem Commun (Camb). 2017 Feb 9;53(13):21

    Type

    Empty backbone

    Use

    Tags

    moxDendra2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTE-mcs

    Plasmid

    #20320

    Depositor

    Dirk Schnappinger

    Article

    Klotzsche et al Nucleic Acids Res. 2009 Apr;37(6):1778-8

    Insert

    multiple cloning site
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 4, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTC-mcs

    Plasmid

    #20317

    Depositor

    Dirk Schnappinger

    Article

    Klotzsche et al Nucleic Acids Res. 2009 Apr;37(6):1778-8

    Insert

    multiple cloning site
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJK611

    Plasmid

    #72532

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit (AaPurQ); large subunit (AaPurL)

    Depositor

    T. J. Kappock

    Inserts

    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 and n/a

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJK626

    Plasmid

    #72461

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Cys/Tyr154>Phe mutant (AaPurE1-S57C,Y154F)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Mutation changes serine-57 to cysteine, tyrosine-…

    Promoter

    T7

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJK691

    Plasmid

    #72381

    Purpose

    Produces Escherichia coli BolA, N-terminal His6 tag (H6EcBolA)

    Depositor

    T. J. Kappock

    Insert

    stationary-phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC (bolA Escherichia coli str. K-12 substr. MG1655)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJK292

    Plasmid

    #73371

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/His89>Asn mutant (AaPurE1-H59N,H89N)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changed histidine-59 to asparagine and histidine-…

    Promoter

    T7

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJK293

    Plasmid

    #73372

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Phe/His89>Phe mutant (AaPurE1-H59F,H89F)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changed histidine-59 to phenylalanine and histidi…

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJK294

    Plasmid

    #73373

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asp/His89>Asp mutant (AaPurE1-H59D,H89D)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changed histidine-59 to aspartate and histidine-8…

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge