We narrowed to 1,530 results for: CAG promoter

1. psiCheck2-SV40p-mtIF3(SR)-3xFLAG-HSVTKp-mCherry

   Plasmid

   #182381

   Purpose

   Dual expression construct encoding shRNA-resistant mtIF3 and mCherry from separate promoters

   Depositor

   Chunghun Lim

   Article

   Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

   Inserts

   mtIF3 (Mtif3 Mouse)
   mCherry
   

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Changed CDS sequence from 'ccacgttcaagtcacga…

   Promoter

   HSV TK promoter and SV40 promoter

   Available Since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLQ1371-sgSTAT3-1

   Plasmid

   #121425

   Purpose

   sgSTAT3-1 sequence: GTCAGGATAGAGATAGACCAG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

   Depositor

   Stanley Qi, Ovijit Chaudhuri

   Article

   Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

   Insert

   sgSTAT3-1
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available Since

   April 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMKO.1-shSpc25

   Plasmid

   #160960

   Purpose

   Spc25 shRNA in pMKO.1 retroviral vector

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Spc25 shRNA (Spc25 Mouse)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLQ1371-sgYAP-10

   Plasmid

   #121424

   Purpose

   sgYAP-10 sequence: GTGCTGTCCCAGATGAACGTC. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

   Depositor

   Stanley Qi, Ovijit Chaudhuri

   Article

   Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

   Insert

   sgYAP-10 (GTGCTGTCCCAGATGAACGTC)
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available Since

   April 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. ATP1A1_G8_Dual_sgRNA

   Plasmid

   #178104

   Purpose

   Coselection for PE3 in human cells. Vector for tandem expression of ATP1A1 G8 sgRNA with a user-specified PE3 nick sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G8 sgRNA + user-specified sgRNA
   

   Use

   CRISPR; Prime editing

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSC43

   Plasmid

   #104817

   Purpose

   CRISPR/Cas9 1xplex gRNA targeting Glyma.04g057400 (Dcl3a-1). Also expresses Cas9 from Gmubi promoter from rolD promoter

   Depositor

   Robert Stupar

   Article

   Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

   Insert

   Glyma.04g057400
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Feb. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. ATP1A1_G3_MTOR_Nick_Intron-45_Dual_sgRNA

   Plasmid

   #178099

   Purpose

   Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick intron-45 sgRNAs from two independent U6 promoters.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G3 + MTOR nick intron-45 sgRNAs
   

   Use

   CRISPR; Prime editing

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. ATP1A1_G4_Q118R_MTOR_F2108L_Dual_pegRNA

   Plasmid

   #178102

   Purpose

   Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and MTOR-F2108L pegRNAs from two independent U6 promoters.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G4 Q118R pegRNA + MTOR-F2108L pegRNA
   

   Use

   CRISPR; Prime editing

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. ATP1A1_G4_Q118R_MTOR_I2017T_Dual_pegRNA

   Plasmid

   #178103

   Purpose

   Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and MTOR-I2017T pegRNAs from two independent U6 promoters.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G4 Q118R pegRNA + MTOR-I2017T pegRNA
   

   Use

   CRISPR; Prime editing

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. ATP1A1_G8_MTOR_Nick_Exon-44_Dual_sgRNA

    Plasmid

    #178105

    Purpose

    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G8 and MTOR Nick exon-44 sgRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G8 + MTOR nick exon-44 sgRNAs
    

    Use

    CRISPR; Prime editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. ATP1A1_G4_Q118R_MTOR_E2419K_Dual_pegRNA

    Plasmid

    #173209

    Purpose

    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and MTOR-E2419K pegRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G4 Q118R pegRNA + MTOR E2419K pegRNA
    

    Use

    CRISPR; Prime editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNdrg4-DsRed

    Plasmid

    #13766

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    Ndrg4 promoter
    

    Use

    Tags

    DsRed2

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. psiCheck2-SV40p-mtIF3(SR)-3xFLAG-CMVp-mito-mTFP1

    Plasmid

    #182380

    Purpose

    Dual expression construct encoding shRNA-resistant mtIF3 and mito-mTFP1 from separate promoters

    Depositor

    Chunghun Lim

    Article

    Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

    Inserts

    mtIF3 (Mtif3 Mouse)
    mito-mTFP1
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Changed CDS sequence from 'ccacgttcaagtcacga…

    Promoter

    CMV promoter and SV40 promoter

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGG198

    Plasmid

    #165605

    Purpose

    Reporter plasmid for B1H-dependent expression of the HIS3/GFP operon with two hEGFP protospacers: PS1(upstream of promoter with 'CAGCG' PAM)-lac-PS2(downstream with 'CGGCG' PAM)-HIS3 and GFP

    Depositor

    Marcus Noyes

    Article

    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

    Insert

    hEGFP protospacer ('CGGCG' PAM) downstream of the HIS3/GFP promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Secondary protospacer ('CGGCG' PAM) ins…

    Promoter

    lac

    Available Since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC-hU6-crBACH1-array_EF1a-BFP

    Plasmid

    #224787

    Purpose

    BACH1 targeting crRNA array for RfxCas13d expressed from multiple hU6 promoter and reporter BFP protein expressed from EF1a promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    crBACH1-1, crBACH1-2, crBACH1-3,
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 and hU6-2xTetO

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pC1300_pUB10_pcoCASphi_E9t_V2_CmYLCVp_35sT_ribozyme_AtPDS3_gRNA10

    Plasmid

    #197958

    Purpose

    T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter and a single AtPDS3 gRNA driven by CmYLCVp and flanked by ribozymes.

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Inserts

    pcoCasphi-2
    AtPDS3 gRNA10
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CmYLCVp and pUBQ10

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. psiCheck2-SV40p-5'UTR-mtIF3(SR)-3xFLAG-3'UTR-CMVp-mito-mTFP1

    Plasmid

    #182378

    Purpose

    Dual expression construct encoding shRNA-resistant mtIF3 and mito-mTFP1 from separate promoters

    Depositor

    Chunghun Lim

    Article

    Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

    Inserts

    mtIF3 (Mtif3 Mouse)
    mito-mTFP1
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Changed CDS sequence from 'ccacgttcaagtcacga…

    Promoter

    CMV promoter and SV40 promoter

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. psiCheck2-SV40p-5'UTR-mtIF3(SR)-3xFLAG-3'UTR-HSVTKp-mCherry

    Plasmid

    #182379

    Purpose

    Dual expression construct encoding shRNA-resistant mtIF3 and mCherry from separate promoters

    Depositor

    Chunghun Lim

    Article

    Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

    Inserts

    mtIF3 (Mtif3 Mouse)
    mCherry
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Changed CDS sequence from 'ccacgttcaagtcacga…

    Promoter

    HSV TK promoter and SV40 promoter

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9 probasin_mTQ2_FlpO

    Plasmid

    #68357

    Purpose

    The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and 8, and SMAD4 ex2 and ex 9 are expressed by the U6 promoter.

    Depositor

    Jannik Elverløv-Jakobsen

    Inserts

    5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9
    FlpO recombinase
    

    Use

    CRISPR

    Tags

    mTurquoise2 (BFP)

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and synthetic Probasin ARRx2

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ATP1A1_G3_MTOR_Nick_Exon-53_Dual_sgRNA

    Plasmid

    #173211

    Purpose

    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick exon-53 sgRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G3 sgRNA + MTOR Nick exon-53 sgRNA
    

    Use

    CRISPR; Prime editing (pe3)

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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