We narrowed to 1,166 results for: ncl

1. pPMEL-Nluc-hbb

   Plasmid

   #239862

   Purpose

   Template for in vitro transcription of secreted nanoluciferase, with signal peptide from PMEL, flanked by the human haemoglobin beta 5' and 3' UTRs.

   Depositor

   Catherine Jopling

   Article

   Smart et al Mol Ther Nucleic Acids. 2025 Jun 24;36(3

   Insert

   Secreted nanoluciferase, with signal peptide from PMEL (gp100), flanked by human haemoglobin beta 5' and 3' UTRs
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   T7 (included as part of insert)

   Available since

   July 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. cBEST4

   Plasmid

   #234660

   Purpose

   Expresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and UGI, Include Golden Gate compatible cassette for sgRNA insertion

   Depositor

   Mingzi M. Zhang

   Article

   Lin et al Appl Environ Microbiol. 2025 Mar 10:e019

   Inserts

   spCas9 cytosine base editor
   Golden Gate compatible sgRNA insertion cassette
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P3 and tcp830

   Available since

   May 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDest17_N-myc

   Plasmid

   #234045

   Purpose

   Bacterial expression of N-terminally 6His-tagged N-myc; includes TEV cleavage site

   Depositor

   Tanja Mittag

   Article

   Yang et al Nat Struct Mol Biol. 2024 Oct;31(10):156

   Insert

   N-myc (MYCN Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDM097

   Plasmid

   #216819

   Purpose

   Aspergillus nidulans codon-adjusted mStayGold fluorescent protein, includes linker for N-terminal or internal tagging.

   Depositor

   Ken Sawin

   Article

   Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

   Insert

   mStayGold
   

   Use

   Tags

   FLAG-(SGGS)x2-XTEN16-(GGGGS)x3 and c4-(GGGGS)x2-X…

   Expression

   Bacterial

   Mutation

   Aspergillus nidulans codon-adjusted

   Promoter

   Available since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEGFPC2-gephyrin C3

   Plasmid

   #199610

   Purpose

   Encodes N-terminal eGFP-tagged gephyrin including the C3 splice cassette for expression in mammalian cells.

   Depositor

   Shiva Tyagarajan

   Article

   Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

   Insert

   Gephyrin (Gphn Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C3 cassette insertion

   Promoter

   CMV

   Available since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEGFPC2-gephyrin C4a

   Plasmid

   #199611

   Purpose

   Encodes N-terminal eGFP-tagged gephyrin including the C4a splice cassette for expression in mammalian cells.

   Depositor

   Shiva Tyagarajan

   Article

   Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

   Insert

   Gephyrin (Gphn Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C4a casette insertion

   Promoter

   CMV

   Available since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLV sgRNA-Notch1 #2 GFP

   Plasmid

   #106951

   Purpose

   Lentivirus encoding sgRNA targeting murine Notch1. sgRNA is less optimal. Includes GFP marker.

   Depositor

   Albert Edge

   Article

   Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

   Insert

   gRNA targeting Notch1 (Notch1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLV sgRNA-Notch1 #3 GFP

   Plasmid

   #106952

   Purpose

   Lentivirus encoding sgRNA targeting murine Notch1. sgRNA is less optimal. Includes GFP marker.

   Depositor

   Albert Edge

   Article

   Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

   Insert

   gRNA targeting Notch1 (Notch1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. DYNLL2

   Plasmid

   #132531

   Purpose

   Human LC8-2, codon optimised for Sf9 expression.

   Depositor

   Andrew Carter

   Article

   Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

   Insert

   DYNLL2 (DYNLL2 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Codon optimised for Sf9 expression

   Promoter

   Available since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. DYNLRB2

    Plasmid

    #132534

    Purpose

    Human Rbl-2, codon optimised for Sf9 expression.

    Depositor

    Andrew Carter

    Article

    Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

    Insert

    DYNLRB2 (DYNLRB2 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Codon optimised for Sf9 expression

    Promoter

    Available since

    Oct. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFA0057

    Plasmid

    #131784

    Purpose

    Guide RNA (gGFP) and Cas9 expression plasmid for cleaving pFA6 series GFP C-terminal tagging cassettes, including GFP-His3MX6. Used to perform CRISPR-Swap of alleles.

    Depositor

    Frank Albert

    Article

    Lutz et al PLoS Genet. 2019 Nov 18;15(11):e1008375.

    Insert

    20mer GFP cassette guide (gGFP) and 5' sgRNA
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTH443-SUP45-G180A

    Plasmid

    #29386

    Depositor

    Tobias von der Haar

    Article

    Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

    Insert

    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    G180A mutant gene including genomic sequence 1432…

    Promoter

    Available since

    June 22, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pTH398-SUP45-G54D

    Plasmid

    #29376

    Depositor

    Tobias von der Haar

    Article

    Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

    Insert

    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    G54D mutant gene including genomic sequence 1432 …

    Promoter

    Available since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pTH372-SUP45-K60A

    Plasmid

    #29379

    Depositor

    Tobias von der Haar

    Article

    Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

    Insert

    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    K60A mutant gene including genomic sequence 1432 …

    Promoter

    Available since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pTH394-SUP45-L34S

    Plasmid

    #29373

    Depositor

    Tobias von der Haar

    Article

    Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

    Insert

    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    L34S mutant gene (corresponding to the sup45-36ts…

    Promoter

    Available since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pTH355-SUP45-T55A

    Plasmid

    #29377

    Depositor

    Tobias von der Haar

    Article

    Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

    Insert

    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    T55A mutant gene including genomic sequence 1432 …

    Promoter

    Available since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pTH373-SUP45-S61A

    Plasmid

    #29380

    Depositor

    Tobias von der Haar

    Article

    Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

    Insert

    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    S61A mutant gene including genomic sequence 1432 …

    Promoter

    Available since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pTH401-SUP45-R65C

    Plasmid

    #29381

    Depositor

    Tobias von der Haar

    Article

    Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

    Insert

    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    R65C mutant gene (corresponding to the sup45-3 al…

    Promoter

    Available since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pTH627-SUP45-V107D

    Plasmid

    #29382

    Depositor

    Tobias von der Haar

    Article

    Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

    Insert

    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    V107D mutant gene including genomic sequence 1432…

    Promoter

    Available since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pTH441-SUP45-K175T

    Plasmid

    #29384

    Depositor

    Tobias von der Haar

    Article

    Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

    Insert

    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    K175T mutant gene including genomic sequence 1432…

    Promoter

    Available since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only