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We narrowed to 15,169 results for: GRN

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Showing: 9201 - 9220 of 15169 results

  3. eSpCas9(1.1)_No_FLAG_ATP1A1_G2

    Plasmid
    #86610
    Purpose
    Expresses the ATP1A1 G2 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 exon 4. Px330-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via nhej using ouabain
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available Since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86610-plasmid-map-sequence-id-168846

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  12. pLKO.1-blast shGAC

    Plasmid
    #110410
    Purpose
    shGAC (Target CCTCTGTTCTGTCAGAGTT), silence glutaminase isoform GAC, blasticidin selection.
    Depositor
    Sandra Martha Gomes Dias
    Insert
    GLS glutaminase (GLS Human)
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Promoter
    U6 (RNA Pol III)
    Available Since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    110410-plasmid-map-sequence-id-218291

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  14. pPBO.ACT006

    Plasmid
    #182716
    Purpose
    Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region, with eGFP for cloning
    Depositor
    Joseph Schoeniger
    Article
    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666
    Insert
    CasRx crRNA cloning backbone
    Use
    CRISPR
    Tags
    GFP in cloning site of crRNA for easier screening…
    Expression
    Bacterial
    Mutation
    Catalytically deactivated R295A, H300A, R849A, H8…
    Promoter
    pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)
    Available Since
    Aug. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    182716-plasmid-map-sequence-id-361297

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  15. pODN-mNG-ANLN

    Plasmid
    #183834
    Purpose
    Repair template for the N-terminal tagging of anillin with mNeonGreen in human cells using CRISPR/Cas9.
    Depositor
    Alisa Piekny
    Article
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Insert
    ANLN homology arms with mNeonGreen-linker (ANLN Human)
    Use
    CRISPR; Donor template
    Tags
    mNeonGreen-linker
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Available Since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    183834-plasmid-map-sequence-id-362443

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  18. pHR-UCOE-SFFV-SID4x-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP

    Plasmid
    #188901
    Purpose
    dCas9 with an N-term Sid4x fusion, a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1) fusion, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element
    Depositor
    Marco Jost, Jonathan Weissman
    Article
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    SID-dCas9-XTEN80-KRAB(Kox1)
    Use
    Lentiviral
    Tags
    HA-2xNLS-XTEN80(linker)-KRAB(Kox1) fusion, P2A-GF…
    Promoter
    SFFV
    Available Since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    188901-plasmid-map-sequence-id-374829

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  19. PLL5.0-Anillin ShRNA-Cherry-Anillin deltaActin binding domain

    Plasmid
    #187276
    Purpose
    Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin delta Actin binding domain
    Depositor
    Alpha Yap
    Article
    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.
    Insert
    Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
    Use
    Lentiviral
    Tags
    mCherry
    Mutation
    deltaActin binding domain
    Promoter
    U6, CMV
    Available Since
    Sept. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    187276-plasmid-map-sequence-id-372976

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Showing: 9201 - 9220 of 15169 results