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M26V DJ-1 pET15b

Plasmid

#60688

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Publication

Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

Insert

M26V DJ-1 (PARK7 Human)

Tags

6xHis

Expression

Bacterial

Mutation

M26V

Promoter

T7

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
mOrange2-Alpha-Actinin-19

Plasmid

#57944

Purpose

Localization: Alpha Actinin, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Publication

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Alpha-Actinin (ACTN1 Human)

Tags

mOrange2

Expression

Mammalian

Promoter

CMV

Available since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pFLAG Caronte (CT#304)

Plasmid

#13860

Depositor

Cliff Tabin

Publication

Yokouchi et al Cell. 1999 Sep 3. 98(5):573-83.

Insert

Caronte (CER1 Chicken)

Tags

flag

Expression

Mammalian

Available since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only

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pAcGP67A-pIgR D1-H6

Plasmid

#12531

Depositor

Pamela Bjorkman

Publication

Hamburger et al Structure. 2004 Nov . 12(11):1925-35.

Insert

human polymeric immunoglobulin receptor (PIGR Human)

Tags

6xHis

Expression

Insect

Mutation

Lys 1 - Val 109

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pAAVS-EGFP^PTC-231

Plasmid

#254989

Purpose

Express EGFP^PTC-231 in mammalian cells

Depositor

Joshua Mendell

Publication

Zhu et al Cell Rep. 2020 Jul 14;32(2):107895. doi:

Insert

EGFP^PTC-231

Expression

Mammalian

Mutation

NA

Available since

May 5, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1alpha-DEST-IRES-Neo

Plasmid

#253766

Purpose

Third generation lentiviral expression Gateway cloning destination vector under a human EF1alpha promoter for expression of a gene of interest linked to neomycin selection by an IRES.

Depositor

Samuel McBrayer

Publication

Savani et al Nat Metab. 2026 Apr 29. doi: 10.1038/s42

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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EGFR-G574V-ET1

Plasmid

#253821

Purpose

Expression of full length EGFR with G574V mutation

Depositor

Timothy Springer

Publication

Watson et al J Biol Chem. 2025 Jul;301(7):110374. doi

Insert

EGFR with G574V mutation (EGFR Human)

Use

Pires2-egfp

Expression

Mammalian

Mutation

G574V

Available since

April 1, 2026

Availability

Academic Institutions and Nonprofits only

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EGFR-R84K-ET1

Plasmid

#253818

Purpose

Expression of full length EGFR with R84K mutation

Depositor

Timothy Springer

Publication

Watson et al J Biol Chem. 2025 Jul;301(7):110374. doi

Insert

EGFR with R84K mutation (EGFR Human)

Use

Pires2-egfp

Expression

Mammalian

Mutation

R84K

Available since

April 1, 2026

Availability

Academic Institutions and Nonprofits only

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pCEFL_GPR161-HA

Plasmid

#249268

Purpose

Expression of GPR161 wild type

Depositor

Ramiro Iglesias-Bartolome

Publication

Krantz et al Cancer Res. 2025 Dec 29. doi: 10.1158/00

Insert

GPR161 (GPR161 Human)

Tags

HA

Expression

Mammalian

Promoter

EF-1

Available since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pET01-NRF1-Ex7-minigene

Plasmid

#237459

Purpose

Vector for constitutive expression of NRF1 alternative exon-7 minigene

Depositor

Thomas Gonatopoulos-Pournatzis

Publication

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Insert

NRF1 ∆exon-7 minigene

Expression

Mammalian

Promoter

RSV LTR

Available since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pLD1112 TRE-mCherry-SKL-22Xm6A MS2

Plasmid

#235128

Purpose

mCherry reporter plasmid for live cell visualization of RNA m6A modification

Depositor

Qin Peng

Publication

Li et al Sci Adv. 2024 Nov 15;10(46):eadp5689. do

Insert

TRE-mCherry-SKL-22Xm6A MS2

Use

Lentiviral

Expression

Mammalian

Available since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pRCA594 - pBA904 Puro-T2A-BFP

Plasmid

#238170

Purpose

Lentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

Depositor

Thomas Norman

Publication

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

Non-targeting sgRNA

Use

CRISPR and Lentiviral

Tags

mTagBFP2

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3 FLAG FBXO31

Plasmid

#236429

Purpose

transient overexpression of FBXO31 in mammalian cells

Depositor

Michele Pagano

Publication

Duan et al Cell Rep. 2021 Oct 19;37(3):109870. doi:

Insert

FBXO31 (FBXO31 Human)

Tags

FLAG

Expression

Mammalian

Available since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pETCK3 TGP-E

Plasmid

#236255

Purpose

Expresses TGP-E, a thermostable fluorescent protein

Depositor

Natasha DeVore

Publication

Anderson et al Biochem Mol Biol Educ. 2024 Sep-Oct;52(5

Insert

Thermal Green Protein Q66E (TGP-E)

Tags

His tag

Expression

Bacterial

Promoter

lac operon

Available since

May 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCDH-CB-Cdkn1a

Plasmid

#228914

Purpose

Expression CDKN1A in mammalian cells

Depositor

Sika Zheng

Publication

Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

Insert

Cdkn1a (Cdkn1a Mouse)

Expression

Mammalian

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-mCGNL1-Myc

Plasmid

#236514

Purpose

For expression of Paracingulin (mouse) in mammalian cells

Depositor

Sandra Citi

Publication

Vasileva et al J Biol Chem. 2022 Apr;298(4):101797. doi

Insert

CGNL1 (Cgnl1 Mouse)

Tags

GFP and Myc

Expression

Mammalian

Mutation

WT

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-RAD52-∆C

Plasmid

#235677

Purpose

Expresses HA-tagged mutant human RAD52 in mammalian cells with a C-terminal deletion (Δ302-410 amino acids).

Depositor

Simon Powell

Publication

Jalan et al Nat Commun. 2024 Sep 5;15(1):7776. doi:

Insert

RAD52 (RAD52 Human)

Tags

HA

Expression

Mammalian

Mutation

deleted amino acids 302-410

Available since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAIP4_2ESK

Plasmid

#234232

Purpose

Heterologous protein expression of ubiquitin-conjugating enzyme E2 D2 in Escherichia coli

Depositor

Christopher Bahl

Publication

Cárcamo Noriega et al Protein Sci. 2026 Sep;35(9):e70746. doi:

Insert

2ESK (UBE2D2 Human)

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pET-22b(+) pCopA RBS30 sfgfp

Plasmid

#226371

Purpose

Plasmid carries copper sensor circuit genes for whole-cell sensing of copper ions with RBS30

Depositor

Urartu Seker

Publication

Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

Insert

sfGFP

Tags

6x HisTag

Expression

Bacterial

Promoter

copA promoter (pCopA) and RBS30 regulation

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGIG-CDKN1A

Plasmid

#228912

Purpose

Expression CDKN1A in mammalian cells

Depositor

Sika Zheng

Publication

Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

Insert

Cdkn1a (Cdkn1a Mouse)

Expression

Mammalian

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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