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pMpGWB100

Plasmid

#68062

Purpose

MCS-containing binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB328

Plasmid

#68656

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

TagRFP

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pCVL SFFV HA.NLS.I-AniIY2K227M(reo).T2A.TagBFP

Plasmid

#31478

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

SFFV HA NLS Y2 K227M T2A BFP

Use

Lentiviral

Tags

HA and NLS

Mutation

F13Y, S111Y, K227M = Y2 nickase "reo" …

Available Since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

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pSV(X) C/EBPbeta

Plasmid

#13393

Depositor

Richard Schwartz

Article

Hu et al J Immunol. 1998 Mar 1. 160(5):2334-42.

Insert

C/EBPbeta (Cebpb Rat)

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only
pUM31dao

Plasmid

#87242

Purpose

Plastid expression cassette containing Schizosaccharomyces pombe D-amino acid oxidase gene. Provides dual positive (D-alanine) and negative (D-valine) selection in transplastomic plants

Depositor

Anil Day

Article

Gisby et al Plant Physiol. 2012 Dec;160(4):2219-26.

Insert

D-amino oxidase

Expression

Plant

Promoter

plastid rrn

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pFIN-XOPS-tdTOM

Plasmid

#44359

Depositor

Susan Semple-Rowland

Article

Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

Insert

XOPS-tdTOM

Use

Lentiviral

Promoter

Xenopus Opsin (XOPS) (pos 3125-4501)

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pFIN-EF1a-GFP-WPRE

Plasmid

#44352

Depositor

Susan Semple-Rowland

Article

Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

Insert

EF1a-GFP

Use

Lentiviral

Promoter

human elongation factor -1a (pos 2526-3969)

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pFGAMS-mMaple3

Plasmid

#154912

Purpose

Expresses human PFAS-mMaple3 fusion in mammalian cells

Depositor

Stephen Benkovic

Article

Chan et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

Insert

PFAS (PFAS Human)

Tags

mMaple3

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pSV(X) C/EBPalpha

Plasmid

#13392

Depositor

Richard Schwartz

Article

Hu et al J Immunol. 1998 Mar 1. 160(5):2334-42.

Insert

C/EBPalpha (Cebpa Rat)

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only
Pr-deGFP-MGapt

Plasmid

#67734

Purpose

OR2-OR1-Pr promoter expressing deGFP-MG aptamer fusion

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-deGFP-MGapt-T500

Use

Synthetic Biology

Expression

Bacterial

Promoter

OR2-OR1-Pr

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_EBFP2

Plasmid

#167857

Purpose

PiggyBac compatible plasmid expressing spCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pJL1-PD-4xDQNAT

Plasmid

#128391

Purpose

In vitro expression of H. influenzae protein D with C terminal 4xDQNAT glycosylation sites

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Protein D

Tags

4xDQNAT glycosylation tag and 6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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myc-Orai1 E106A pHAGE

Plasmid

#22752

Depositor

Anjana Rao

Article

Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

Insert

Orai1 E106A (ORAI1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

E106A

Available Since

Jan. 20, 2010

Availability

Academic Institutions and Nonprofits only
FRB-polySH33-EGFP

Plasmid

#175792

Purpose

Bacterial expression and purification, fluorescently tagged scaffold for droplet visualization and volume quantification

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

NCK1 (NCK1 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

tac

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pWZL-Stat5a1*6

Plasmid

#130669

Purpose

Retroviral vector for the expression of constitutive Stat5a (hygromycin resistance)

Depositor

Gerardo Ferbeyre

Article

Mallette et al J Biol Chem. 2007 Nov 30;282(48):34938-4

Insert

Stat5a (Stat5a Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

changed Histidine 298 to Arginine and Serine 710 …

Promoter

LTR

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pMpGWB135

Plasmid

#68589

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

TagRFP

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_KanR neo

Plasmid

#167861

Purpose

PiggyBac compatible plasmid expressing spCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCVL.SFFV.Kozak.HA.NLS.Y2Ani.IRES.BFP

Plasmid

#45578

Purpose

Expresses Y2 variant of I-Ani I nuclease in mammalian cells with BFP tracker

Depositor

Andrew Scharenberg

Article

Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Insert

coY2 Ani

Use

Lentiviral

Tags

HA, IRES BFP, and NLS

Expression

Mammalian

Mutation

co indicates codon optimization for mammalian exp…

Promoter

SFFV

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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NHA-QN

Plasmid

#36441

Depositor

Edward Chan

Article

Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-4

Insert

TNRC6A (TNRC6A Human)

Expression

Mammalian

Mutation

aa 1194-1580 only

Available Since

Oct. 3, 2012

Availability

Academic Institutions and Nonprofits only
NavB4-GFP/pEGFP-N1

Plasmid

#197310

Purpose

Plasmid expressing an antigen targeting the Nav beta4 sodium channel subunit

Depositor

Lori Isom

Article

Aman et al J Neurosci. 2009 Feb 18;29(7):2027-42. d

Insert

NavB4-GFP (Scn4b Rat)

Tags

eGFP

Expression

Mammalian

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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