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pHAGE-EGFR-G719C

Plasmid

#116254

Purpose

Lentiviral expression of EGFR G719C

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Mutation

G719C

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-ERBB3-D297Y

Plasmid

#116360

Purpose

Lentiviral expression of ERBB3 D297Y

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ERBB3 (ERBB3 Human)

Use

Lentiviral

Mutation

D297Y

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_ZBTB24_WT_V5

Plasmid

#83004

Purpose

Gateway Donor vector containing ZBTB24, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

ZBTB24 (ZBTB24 Human)

Use

Gateway Cloning

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-A289T

Plasmid

#116229

Purpose

Lentiviral expression of EGFR A289T

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Mutation

A289T

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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PLEX-PGK-ANXA11-mCerulean

Plasmid

#164214

Purpose

pLEX lentivirus backbone expresses mCerulean tagged ANXA11 under PGK promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Use

Lentiviral

Tags

mCerulean

Promoter

PGK

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLEx-iSeroSnFR

Plasmid

#128486

Purpose

Fluorescent reporter for serotonin (conditional + membrane localization tag)

Depositor

Lin Tian

Article

Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

Has Service

AAV9

Insert

iSeroSnFR

Use

AAV

Tags

Ig-kappa leader, Myc, PDGFR, and cpsfGFP

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-SMO

Plasmid

#116792

Purpose

Lentiviral expression of SMO

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

SMO (SMO Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PDGFRA-Y288C

Plasmid

#116453

Purpose

Lentiviral expression of PDGFRA Y288C

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PDGFRA (PDGFRA Human)

Use

Lentiviral

Mutation

Y288C

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-M766_A767insAI

Plasmid

#116279

Purpose

Lentiviral expression of EGFR M766_A767insAI

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Mutation

M766_A767insAI

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-ALK-R1275Q

Plasmid

#116111

Purpose

Lentiviral expression of ALK R1275Q

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ALK (ALK Human)

Use

Lentiviral

Mutation

R1275Q

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-MAP2K1-K57N

Plasmid

#116427

Purpose

Lentiviral expression of MAP2K1 K57N

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

MAP2K1 (MAP2K1 Human)

Use

Lentiviral

Mutation

K57N

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_ARAF_WT

Plasmid

#82900

Purpose

Gateway Donor vector containing ARAF, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

NM_001654 (ARAF Human)

Use

Gateway Cloning

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CA-Q546K

Plasmid

#116527

Purpose

Lentiviral expression of PIK3CA Q546K

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Mutation

Q546K

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-MAP2K1

Plasmid

#116757

Purpose

Lentiviral expression of MAP2K1

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

MAP2K1 (MAP2K1 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-N581S

Plasmid

#116188

Purpose

Lentiviral expression of BRAF N581S

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

N581S

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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DNA-CARzeta-GFP

Plasmid

#89344

Purpose

2nd generation lentiviral vector which expresses DNA-CAR receptor fused to GFP

Depositor

Ron Vale

Article

Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

Insert

DNA-CARzeta (CD247, CD86 Human)

Use

Lentiviral

Tags

SNAPf tag and mEGFP

Expression

Mammalian

Available Since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_TERT_p.L593F

Plasmid

#82856

Purpose

Gateway Donor vector containing TERT, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

TERT (TERT Human)

Use

Gateway Cloning

Mutation

D516G, L593F

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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ATX3-77Q

Plasmid

#184249

Purpose

Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)

Depositor

Sandra Macedo Ribeiro

Article

Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111

Insert

Ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track (ATXN3 Human, Synthetic)

Tags

6x His-Tag and TEV cleavage site

Expression

Bacterial

Mutation

Codon optimization for protein expression in BL2…

Promoter

T7 promoter

Available Since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-L62R

Plasmid

#116265

Purpose

Lentiviral expression of EGFR L62R

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Mutation

L62R

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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Mouse Alpha2 delta-2 pMT2

Plasmid

#58732

Purpose

expression of mouse Alpha2 delta-2 in mammalian cells

Depositor

Annette Dolphin

Article

Barclay et al J Neurosci. 2001 Aug 15;21(16):6095-104.

Insert

alpha2 delta-2 (Cacna2d2 Mouse)

Expression

Mammalian

Promoter

SV40

Available Since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only

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