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B6-3

Plasmid

#111487

Purpose

Ser-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Ser-tRNA synthetase

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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DS-TDcasN-

Plasmid

#48660

Purpose

Bacterial nuclease-null TD Cas9 expression

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Inserts

Cas9, nuclease-null
TD tracrRNA

Use

CRISPR

Mutation

D to A, DH to AA, N to A

Promoter

proC and tracrRNA promoter

Available Since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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pJL009

Plasmid

#122862

Purpose

Binary expression vector containing 35S:MS2-SRDX:tRBCS followed by GreenGate A-G sequences for insertion of additional modules

Depositor

Markus Schmid

Article

Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

Insert

35S:MS2-SRDX:tRBCS

Use

Golden gate compatible plant transformation vector

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pShuttle-CMV-EGFP-C

Plasmid

#13887

Depositor

Lorene Lanier

Article

Strasser et al Neuron. 2004 Jul 8. 43(1):81-94.

Insert

C domain of N-WASP (WASL Bovine)

Use

Adenoviral

Tags

EGFP

Mutation

The BglII site in the MCS of pShuttle-CMV was fil…

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only

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pJL1-FETCH1-StrepII

Plasmid

#210648

Purpose

In vitro expression of FETCH reporter from the T7 promoter

Depositor

Michael Jewett

Article

Willi et al ACS Synth Biol. 2024 Jul 19;13(7):2253-2

Insert

FETCH1

Use

Synthetic Biology

Tags

Strep-TagII

Promoter

T7

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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B5-2

Plasmid

#111481

Purpose

Arg-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Arg-tRNA synthetase

Tags

6xHis

Expression

Bacterial

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCB6-ZONAB-A

Plasmid

#136641

Purpose

Expression of ZONAB isoform A in mammalian cells

Depositor

Maria Balda, Karl Matter

Article

Balda et al EMBO J. 2000 May 2;19(9):2024-33. doi: 1

Insert

ZONAB isoform A (YBX3 Canine)

Expression

Mammalian

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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NM R(delta) 2-5 GFP

Plasmid

#1209

Depositor

Susan Lindquist

Article

Liu et al Nature 1999 Aug 5;400(6744):573-6.

Insert

sup35 NM domain (SUP35 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

last four repeats of sup35 N domain deleted

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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pCaSpeR-act5cB-LexAQF

Plasmid

#61310

Purpose

Expresses chimeric transactivator LexA_DBD:QF2_AD in Drosophila

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

LexA_DBD::QF2_AD

Expression

Insect

Available Since

Jan. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pBbA7a-RFP

Plasmid

#35316

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pRK5-mWnt8b

Plasmid

#42285

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Wnt8b (Wnt8b Mouse)

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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PM-SP!TB

Plasmid

#48650

Purpose

Bacterial SP crRNA expression: targets SP to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial SP crRNA to prototspacer B

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pET28a-ChmN

Plasmid

#221348

Purpose

Express ChmN in E. coli

Depositor

Emily Balskus

Article

Crooke et al J Am Chem Soc. 2024 Jun 12;146(23):16268

Insert

chmN

Tags

His6

Expression

Bacterial

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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B3-3

Plasmid

#111473

Purpose

Glu-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Glu-tRNA synthetase

Tags

6xHis

Expression

Bacterial

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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JB26

Plasmid

#128920

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

Rab33BQL-BirA*-HA-MAO (RAB33B Human)

Expression

Mammalian

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDD377

Plasmid

#91823

Purpose

Codon-optimized mTurquoise2 donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

mTurquoise2-C1

Use

CRISPR

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP ABR

Plasmid

#30315

Depositor

Alan Fanning

Article

Fanning et al FASEB J. 2002 Nov . 16(13):1835-7.

Insert

Zonula Occludens-1 actin-binding region (TJP1 Human)

Tags

GFP and vsvg

Expression

Mammalian

Mutation

contains only amino acids 1159-1382

Available Since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only
p18S.1 G693A

Plasmid

#51728

Purpose

transcribes pactamycin resistant 18S

Depositor

Vincent Mauro

Article

Burman et al Nucleic Acids Res. 2012 Sep;40(16):8085-

Insert

18S

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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Hec1 mCherry Plk1 T210D

Plasmid

#45205

Depositor

Michael Lampson

Article

Liu et al J Cell Biol. 2012 Aug 20;198(4):491-9. d

Insert

Hec1-targeted mCherry Plk1 T210D

Tags

mCherry

Expression

Mammalian

Mutation

Plk1 T210D mutation

Promoter

T7

Available Since

May 30, 2013

Availability

Academic Institutions and Nonprofits only

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B2-3

Plasmid

#111470

Purpose

Lys-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Lys-tRNA synthetase

Tags

6xHis

Expression

Bacterial

Available Since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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