172,845 results
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pCAGGS-FLAG-CaMKIIA
Plasmid#186825PurposeExpresses FLAG-CaMKIIA in mammalian cellsDepositorAvailable SinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTetOne-tdTomato-cNLS
Plasmid#138529PurposeInducible expression of tdTomato with nuclear localization signalDepositorInserttdTomato
Tags3x SV40 cNLSExpressionMammalianPromoterTRE3GsAvailable SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSH-EF1a-OsTIR1.F74A-mCherry-IRES-puro
Plasmid#216247PurposeExpress OsTIR1.F74A mutant with mCherry tag for AID2DepositorInsertOsTIR1.F74A
UseCRISPR and TALEN; Human safe harbor locus (a avs1…TagsmCherryExpressionMammalianPromoterEF1aAvailable SinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α1.1-FLEX-rc [Jaws-KGC-GFP-ER2]
Plasmid#108274PurposeAAV-mediated expression of Jaws-KGC-GFP-ER2 under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.DepositorInsertJaws-KGC-GFP-ER2
UseAAVTagsER2, GFP, and KGCExpressionMammalianMutationK200R W214FPromoterEF1α promoter (1.1kb short version)Available SinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_DA1h
Plasmid#113050Purposeexpress the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1h in neuronsDepositorHas ServiceAAV Retrograde and AAV9InsertGPCR activation based DA sensor GRAB_DA1h (DRD2 Human)
UseAAVPromoterhSynAvailable SinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
DsRed-Rab5 WT
Plasmid#13050DepositorInsertRAB5A (Canis familiaris) (RAB5A Canis familiaris)
TagsDsRed2 and HAExpressionMammalianMutationasparagine 3 changed to serine (neutral); serine …Available SinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
AiP1858 - pAAV-AiE2538m-minBG-SYFP2-WPRE-BGHpA
Plasmid#214558PurposeAiE2538m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cellsDepositorInsertSYFP2
UseAAVPromoterminBGAvailable SinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-TRIM33
Plasmid#65398PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorAvailable SinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pA-His6-Tn5
Plasmid#171871PurposeExpresses protein A and Tn5 Transposase fusion protein in bacterial cellsDepositorInsertProtein A and hyperactive Tn5 transposase
TagsHis-tagExpressionBacterialMutationWTAvailable SinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEXSISERS_mGreenLantern_FRT-PuroR-HSV-TK
Plasmid#177868PurposeCloning Backbone for mGreenLantern-based EXSISERS containing FRT-sites flanked PuroR-HSV-TK cassette; clone homology arms via BbsI (or BpiI).DepositorInsertmGreenLantern-based EXSISERS
UseCRISPR, Synthetic Biology, and UnspecifiedTagsOLLASExpressionMammalianAvailable SinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-8
Plasmid#163136PurposepLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the LppSP-OmpA surface expression anchor and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterP(rhaB)Available SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
h-alpha-ENaC
Plasmid#83430Purposefull length human alpha ENaC with Flag tagDepositorAvailable SinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSET a sfGCaMP6s-T78H
Plasmid#100023PurposeBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant containing T78H mutationDepositorInsertsfGCaMP6s-T78H
Tags6x HIS tag and Xpress_EK tagExpressionBacterialMutationcpEGFP replaced with superfolder cpGFP and T78H m…PromoterT7Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
M13KO7-ΔPS-ΔgeneIII
Plasmid#134351PurposeHelper phage for M13 phage productionDepositorInsertHelper phage gene
ExpressionBacterialAvailable SinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_WWTR1_WT
Plasmid#82253PurposeGateway Donor vector containing WWTR1 , part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUGI-NLS
Plasmid#101091PurposeExpresses UGI in mammalian cellsDepositorInsertUGI
TagsNLSExpressionMammalianPromoterCMVAvailable SinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
cavin-2-mEGFP
Plasmid#27710DepositorAvailable SinceJuly 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Opto-Alk8
Plasmid#207615PurposeZebrafish Alk8 BMP receptor kinase domain fused to VfLOVDepositorInsertAlk8 (acvr1l) kinase domain + VfLOV domain (acvr1l Vaucheria frigida, Zebrafish)
Available SinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_LaG16_2_synNotch_Gal4VP64
Plasmid#85421PurposeLentiviral vector for expression of LaG16_2 GFP nanobody Gal4VP64 synthetic NotchDepositorInsertLaG16_2 GFP nanobody synNotch Gal4VP64
UseLentiviralPromoterPGKAvailable SinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only
