We narrowed to 18,383 results for: URE

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pHH0103_VAV2-1/2*

Plasmid

#91483

Purpose

Protein expression and purification of human SH3 domain construct VAV2-1/2*

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

VAV2-1/2* (VAV2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_STAC3-1/2

Plasmid

#91271

Purpose

Protein expression and purification of human SH3 domain construct STAC3-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

STAC3-1/2 (STAC3 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-rev_U6_MCS

Plasmid

#109769

Purpose

Multisite gateway vector for 3' expression of a guide RNA from the U6 promoter (cassette in the reverse orientation). Contains BseRI sites for insertion of gRNA sequence

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

U6-gRNA cassette

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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UKNP1.19.2

Plasmid

#98193

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 DDX5

Plasmid

#67111

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

DDX5 (DDX5 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pHH0103_SNX33-1/1

Plasmid

#91278

Purpose

Protein expression and purification of human SH3 domain construct SNX33-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SNX33-1/1 (SNX33 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pET30a-SRAP

Plasmid

#84524

Purpose

Expresses murine SRAP domain (250 aa) in bacteria

Depositor

Douglas Feldman

Article

Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

Insert

SRAP domain (Hmces Mouse)

Tags

6xHis and S-Tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 18, 2016

Availability

Academic Institutions and Nonprofits only

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BirA_BVES untagged

Plasmid

#196581

Purpose

expresses BirA

Depositor

Cheryl Arrowsmith

Article

Alteen et al Structure. 2023 Sep 7;31(9):1121-1131.e6

Insert

BirA_Ecoli

Use

Baculovirus expression

Available Since

June 9, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pTS1026-Tier1-PhCMV-3xNLS-iRFP670

Plasmid

#169532

Purpose

Tier-1 vector encoding PhCMV-driven iRFP670 that is localized to the nucleus (PhCMV-3xNLS-iRFP670-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven nucleus-localized far-red fluorescent protein iRFP670

Expression

Mammalian

Promoter

PhCMV

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDESTFLAGHApuro3-Ago2K533AK570A

Plasmid

#14104

Depositor

Thomas Tuschl

Article

Ma et al Nature. 2005 Mar 31. 434(7033):666-70.

Insert

Ago2 (AGO2 Human)

Tags

FLAG/HA

Expression

Mammalian

Mutation

changed Lys533 to Ala, Lys570 to Alanine

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pRSETa-Amrose v3

Plasmid

#79539

Purpose

pRSETa (Invitrogen) plasmid expressing Amrose variant 3

Depositor

Randolph Caldwell

Article

Schoetz et al PLoS One. 2014 Sep 5;9(9):e107069. doi:

Insert

Amrose v3

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Bacterial

Promoter

T7

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pXylA-RgTALsyn

Plasmid

#100948

Purpose

Expression of Tyrosine Ammonia Lyase from constitutive XylA promoter

Depositor

Mattheos Koffas

Article

Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

Insert

RgTALsyn

Use

Synthetic Biology

Expression

Bacterial

Mutation

Silent mutation to remove internal NdeI site on T…

Promoter

XylA (Constitutive)

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR sgSLC19A1

Plasmid

#102314

Purpose

genetic depletion of SLC19A1

Depositor

David Sabatini

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

sgSLC19A1 (SLC19A1 Human)

Use

Lentiviral

Promoter

U6

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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PM18033-PLS-KP6-A66S_rpoA-U

Plasmid

#190989

Purpose

Test the U-to-C editing activity of KP6:A66S on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Tags

His

Expression

Mammalian

Mutation

DYW-KP:A66S

Promoter

CMV

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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PM18033-PLS-KP2-A66S_rpoA-U

Plasmid

#190977

Purpose

Test the U-to-C editing activity of KP2:A66S on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP2

Tags

His

Expression

Mammalian

Mutation

DYW-KP:A66S

Promoter

CMV

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-Mac-GFP

Plasmid

#58853

Purpose

AAV-mediated expression of Mac-GFP under the Syn promoter

Depositor

Edward Boyden

Article

Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Insert

Mac-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

Syn

Available Since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only

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-962 human cyclin D1 promoter EtsC site mutant pGL3Basic

Plasmid

#32731

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

CCND1 promoter (CCND1 Human)

Use

Luciferase

Mutation

-424 EtsC site TTCCT deletion

Promoter

-962 CCND1 promoter

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
RBM45_Full-length_pGEX

Plasmid

#135127

Purpose

Recombinant expression of RBM45 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

RBM45 (RBM45 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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UKNP3.2.1

Plasmid

#98222

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 IFNG

Plasmid

#67056

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

IFNG (IFNG Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only