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pDP0437

Plasmid

#221127

Purpose

Plasmid for generating integration cassette to C-terminally tag gene of interest with pHluorin::HPHMX

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

pHluorin

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV_Myc-CylM-P2A-FLAG-YFast-TEV-His-CylLL-S15T

Plasmid

#225063

Purpose

Expression of CylM-modified CylLL-S15T in human cells

Depositor

Wilfred van der Donk

Article

Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

Insert

CylLL-S15T

Tags

Multiple tags due to polycistronic expression

Expression

Mammalian

Mutation

S15T

Promoter

CMV

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_CD8alpha variant 2

Plasmid

#188362

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2+-H2B-pr-mEosFP

Plasmid

#141101

Purpose

H2B fused to pr-mEosFP for chromatin visualization and primed conversion

Depositor

Periklis Pantazis

Article

Welling et al Bio Protoc. 2020 Jun 5;10(11):e3645. doi

Insert

Histone 2B

Tags

pr-mEosFP

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-cofilin S3A

Plasmid

#163604

Purpose

Expresses constitutively active cofilin in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

cofilin1 (Cfl1 Mouse)

Expression

Mammalian

Mutation

S3A substitution to make cofilin constitutively a…

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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Cytosolic Syk Biosensor

Plasmid

#125729

Purpose

FRET biosensor for studying SYK activities in cells

Depositor

Yingxiao Wang

Article

Xiang et al Cell Mol Bioeng. 2011 Dec;4(4):670-677.

Insert

SYK biosensor (ZAP70 Synthetic)

Tags

6xHistine Tag at the N-terminal.

Expression

Mammalian

Available Since

Nov. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pX330-PCNT

Plasmid

#227284

Purpose

Expresses SpCas9 and a sgRNA targeting the N-terminus of PCNT for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of PCNT (PCNT Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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PL-SIN-PGK-EiP

Plasmid

#21312

Purpose

Control Lentiviral vector with ubiquitous PGK promoter, expresses EGFP and Puro resistance

Depositor

James Ellis

Article

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

Enhanced Green Fluorescence Protein, Puromycin resistance gene

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_hsNotch1 LNR

Plasmid

#188336

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch1-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_Notch1-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pSLQ10531

Plasmid

#239267

Purpose

Expresses NLS-dCas13b-ABI for CRISPR-TO perturbation in the nucleus of cell lines

Depositor

Stanley Qi

Article

Han et al Nature. 2025 Jul;643(8070):241-251. doi:

Insert

NLS-dPspCas13b-EGFP-ABI

Use

CRISPR and Lentiviral

Tags

Flag tag and V5 tag

Expression

Mammalian

Mutation

H133A and H1058A in PspCas13b

Promoter

EF1a

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-Limk1

Plasmid

#163414

Purpose

Expresses LIMK1 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Limk1 (Limk1 Mouse)

Expression

Mammalian

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(1)-T2A-Gal4-Hsp70

Plasmid

#62897

Purpose

Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 1.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pMXs-Klf4-IP

Plasmid

#15920

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

Kruppel-like factor 4 (gut) (Klf4 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

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pPRIME-CMV-dsRed-FF3

Plasmid

#11664

Purpose

3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a CMV promoter controlling expression of dsRed and miR30-based shRNA targeting firefly luciferase

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Insert

FF3

Use

Lentiviral

Tags

dsRed

Expression

Mammalian

Available Since

March 1, 2007

Availability

Academic Institutions and Nonprofits only
pFastBac HT JS-Munc18c

Plasmid

#135555

Purpose

Express mouse Munc18c in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18c.

Depositor

Jingshi Shen

Article

Yu et al Methods Mol Biol. 2019;1860:237-249. doi

Insert

Munc18c

Tags

6xHis tag and TEV site

Expression

Insect

Promoter

polyhedrin promoter

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCOM-CD63-COM

Plasmid

#138350

Purpose

Expressing COM-CD63-COM fusion protein in mammalian cells

Depositor

Baisong Lu

Article

Yao et al J Extracell Vesicles. 2021 Mar;10(5):e12

Insert

COM-CD63-COM fusion protein

Use

CRISPR and Lentiviral

Tags

COM is fused to the N and C termini of human CD63

Expression

Mammalian

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPguCas13b-3xEGFP-NLS-3xFlag

Plasmid

#132399

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPguCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H151A; H1121A

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(1)-T2A-p65AD-Hsp70

Plasmid

#62914

Purpose

Creating p65AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 1

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-p65AD-Hsp70

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

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pHR- flag-Lag16-Ecad

Plasmid

#205197

Purpose

This vector encodes of the synCAM (Ecad) and GFP nanobody LAG16

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM Ecad and Lag16 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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3xmNeonGreen-3xrGBD

Plasmid

#176101

Purpose

Visualization of active Rho

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

Insert

rhotekin (Rtkn Mouse)

Expression

Mammalian

Promoter

CMVdel

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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