We narrowed to 42,822 results for: ANT;

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pcDNA3.1/CALR ins5 (del1-197)-FLAG

Plasmid

#204534

Purpose

Mammalian expression of human CALR ins5 (del1-197)-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR ins5 (del1-197)-FLAG (CALR Human)

Expression

Mammalian

Mutation

ins5 (del1-197)

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pFUGW-shRIIα-RIIαS97A-IRES-EGFP

Plasmid

#183457

Purpose

Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97A variant

Depositor

Matthew Gold

Article

Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

Insert

sgRNA and Prkar2a (Prkar2a Rat)

Use

Lentiviral

Mutation

shRNA-resistant mutations: C135>G, G138>A, …

Promoter

shRNA: H1 / gene: ubiquitin

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pFUGW-shRIIα-RIIαS97E-IRES-EGFP

Plasmid

#183458

Purpose

Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97E variant

Depositor

Matthew Gold

Article

Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

Insert

sgRNA and Prkar2a (Prkar2a Rat)

Use

Lentiviral

Mutation

shRNA-resistant mutations: C135>G, G138>A, …

Promoter

shRNA: H1 / gene: ubiquitin

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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TMD18

Plasmid

#163673

Purpose

Expresses a ST6GAL1 mutant with 18 amino acids in its TMD in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)

Tags

GFP

Expression

Mammalian

Mutation

For this partial length ST chimera, two restricti…

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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TMD19

Plasmid

#163674

Purpose

Expresses a ST6GAL1 mutant with 19 amino acids in its TMD in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)

Tags

GFP

Expression

Mammalian

Mutation

For this partial length ST chimera, two restricti…

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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TMD20

Plasmid

#163675

Purpose

Expresses a ST6GAL1 mutant with 20 amino acids in its TMD in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)

Tags

GFP

Expression

Mammalian

Mutation

For this partial length ST chimera, two restricti…

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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TMD21

Plasmid

#163676

Purpose

Expresses a ST6GAL1 mutant with 21 amino acids in its TMD in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)

Tags

GFP

Expression

Mammalian

Mutation

For this partial length ST chimera, two restricti…

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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TMD22

Plasmid

#163677

Purpose

Expresses a ST6GAL1 mutant with 22 amino acids in its TMD in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)

Tags

GFP

Expression

Mammalian

Mutation

For this partial length ST chimera, two restricti…

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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TMD24

Plasmid

#163678

Purpose

Expresses a ST6GAL1 mutant with 24 amino acids in its TMD in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)

Tags

GFP

Expression

Mammalian

Mutation

For this partial length ST chimera, two restricti…

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR/D-Topo genomic A. thaliana IMPORTIN ALPHA 4

Plasmid

#175815

Purpose

pENTR plasmid with genomic sequence of Arabidopsis thaliana IMPORTIN ALPHA ISOFORM 4 (AT1G09270) without stop codon for C-terminal epitope tags

Depositor

Mark Banfield, Lennart Wirthmueller

Article

Wirthmueller et al Plant J. 2015 Jan;81(1):40-52. doi: 10.1

Insert

IMPORTIN ALPHA ISOFORM 4 (IMPA-4 Mustard Weed)

Use

Pentr plasmid for gateway cloning

Tags

none

Promoter

none

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR/D-Topo CDS A. thaliana IMPORTIN ALPHA 2

Plasmid

#175816

Purpose

pENTR plasmid with CDS of Arabidopsis thaliana IMPORTIN ALPHA ISOFORM 2 (AT4G16143.1) without stop codon for C-terminal epitope tags

Depositor

Mark Banfield, Lennart Wirthmueller

Article

Wirthmueller et al Plant J. 2015 Jan;81(1):40-52. doi: 10.1

Insert

IMPORTIN ALPHA ISOFORM 2 (IMPA-2 Mustard Weed)

Use

Pentr plasmid for gateway cloning

Tags

none

Mutation

natural polymorphism P65S, annotated in Genbank f…

Promoter

none

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR/D-Topo CDS A. thaliana IMPORTIN ALPHA 9

Plasmid

#175817

Purpose

pENTR plasmid with CDS of Arabidopsis thaliana IMPORTIN ALPHA ISOFORM 9 (AT5G03070.1) without stop codon for C-terminal epitope tags

Depositor

Mark Banfield, Lennart Wirthmueller

Article

Wirthmueller et al Plant J. 2015 Jan;81(1):40-52. doi: 10.1

Insert

IMPORTIN ALPHA ISOFORM 9 (IMPA-9 Mustard Weed)

Use

Pentr plasmid for gateway cloning

Tags

none

Promoter

none

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ239-STU

Plasmid

#138112

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized FnCas12a without promoter and terminator

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

FnCas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pPGK-T7/2-CD44v2-10 (-cyt)

Plasmid

#137825

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44v2-10 (-cyt) (CD44 Human)

Tags

GFP

Expression

Mammalian

Mutation

without cytoplasmic region - N213S on the CD44 re…

Promoter

PGK

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 sgRNA sCRNA 2.0 (GB2461)

Plasmid

#160593

Purpose

Version of the native Cas9-sgRNA with one native WT aptamer sequence and F6 aptamer sequence recognized for Ms2 coat protein, in 3'.

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

sgRNA sCRNA 2.0

Use

CRISPR

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 sgRNA:scaffold tetraloop MS2 aptamer SAM (GB1436)

Plasmid

#160570

Purpose

A version of scaffold sgRNA with the sequence of MS2 aptamer inside the tetraloop of the scaffold

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

sgRNA:scaffold tetraloop MS2 aptamer SAM

Use

CRISPR

Mutation

BsaI and BsmBI sites removed

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX304-ARF5-T161A-mNeonGreen

Plasmid

#162026

Purpose

Fast cycling ARF mutant

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

ARF5 (ARF5 Human)

Use

Lentiviral

Mutation

T161A

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLL5 sgRNA(MS2)-MS2-hA3a D131A

Plasmid

#112131

Purpose

empty sgRNA cloning vector with MS2-humanA3a D131A

Depositor

Fei-Long Meng

Article

Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

Insert

human Apobec3a D131A mutant (APOBEC3A Human)

Use

CRISPR

Expression

Mammalian

Mutation

D131A mutant in hA3a

Promoter

hU6,CBh

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-eFFly-mCherry

Plasmid

#104833

Purpose

3rd generation lentiviral vector. eFFly luciferase for in vivo Imaging, mCherry for fluorescent detection

Depositor

Irmela Jeremias

Article

Ebinger et al Cancer Cell. 2016 Dec 12;30(6):849-862.

Inserts

eFFly
mCherry

Use

Lentiviral and Luciferase

Expression

Mammalian

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pnCS_SEPT7_SEPT9_i1-TEV-Strep

Plasmid

#174500

Purpose

bacterial co-expression of human SEPT7 and of human SEPT9_i1

Depositor

Manos Mavrakis

Article

Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

Inserts

SEPTIN7 (SEPTIN7 Human)
SEPTIN9 (SEPTIN9 Human)

Tags

TEV-Strep

Expression

Bacterial

Available Since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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