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pRC01

Plasmid

#114680

Purpose

sfGFP.Strands 1-9

Depositor

Matthew Good

Article

Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

Insert

sfGFP Strands 1-9

Tags

GST

Expression

Bacterial

Promoter

T7

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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HA Raptor mutant 9

Plasmid

#8476

Depositor

David Sabatini

Article

Kim et al Cell 2002 Jul 26;110(2):163-75.

Insert

Raptor (RPTOR Human)

Tags

ha

Expression

Mammalian

Mutation

1191RVYDRR1196-DAAADD (R1191D, V1192A, Y1193A, D1…

Available Since

June 20, 2005

Availability

Academic Institutions and Nonprofits only
pPD535 VP16-ZF129

Plasmid

#138768

Purpose

Constitutive expression of VP16-ZF129 under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-ZF129

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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rNL1(46-638)

Plasmid

#20172

Depositor

Axel Brunger

Article

Arac et al Neuron. 2007 Dec 20. 56(6):992-1003.

Insert

Neuroligin 1 (Nlgn1 Rat)

Tags

C-terminal 6X His tag

Expression

Insect

Mutation

no splice sites

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pCellFree_G10

Plasmid

#67144

Purpose

Gateway destination vector for cell-free expression with C-term sfGFP 8xHis tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

HIS and sfGFP

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pPD303 VP64-ZF43

Plasmid

#138835

Purpose

Constitutive expression of VP64-ZF43 under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP64-ZF43

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pPP378

Plasmid

#158797

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-1(D371A) in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

dCasPhi-1 (D371A)

Expression

Bacterial

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pDGB1_alpha2

Plasmid

#68225

Purpose

GoldenBraid level 1 Alpha plant expression vector.

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pDGB1_omega2

Plasmid

#68235

Purpose

GoldenBraid level 1 Omega plant expression vector

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pPD158 dsRedExpress2-ZFi37

Plasmid

#138797

Purpose

Constitutive expression of dsRedExpress2-ZFi37 under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

dsRedExpress2-ZFi37

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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MLS- mir-17-19b

Plasmid

#64089

Purpose

expresses a truncated version of mir-17-92 lacking miR-92

Depositor

Lin He

Article

Olive et al Genes Dev. 2009 Dec 15. 23(24):2839-49.

Insert

mir-17-19b (MIR17 Human)

Expression

Mammalian

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only
pKM394

Plasmid

#134211

Purpose

Baculovirus expression of CENP-N and CENP-L

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

CENP-N-his-CENP-L

Expression

Insect

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pSLQ2853-5 pHR: U6-Sasgv2CXCR4-3 CMV-EGFP

Plasmid

#84256

Purpose

Expresses optimized Sa sgCXCR4-3 gRNA with an EGFP fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sa sgCXCR4-3

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pJH291

Plasmid

#172153

Purpose

Reporter plasmid for pylBCD variant in pCDF backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para sfGFP.2TAG; Plpp pylS; PproK pylT; AraC

Expression

Bacterial

Mutation

sfGFP(N39*, Y151*)

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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HA Raptor mutant 4

Plasmid

#8474

Depositor

David Sabatini

Article

Kim et al Cell 2002 Jul 26;110(2):163-75.

Insert

Raptor (RPTOR Human)

Tags

ha

Expression

Mammalian

Mutation

391SQ392-PA (S391P, Q392A)

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pPOTv6-blast-3Myc:HaloTag:3Myc

Plasmid

#181943

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

HaloTag

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pSG5-FLAG-CaMKKalpha FL WT

Plasmid

#33327

Depositor

Anthony Means

Article

Green et al Cell Signal. 2011 Dec;23(12):2005-12. Ep

Insert

Calcium/Calmodulin dependent protein kinase kinase alpha (Camkk1 Rat)

Tags

FLAG

Expression

Mammalian

Promoter

SV40

Available Since

March 16, 2012

Availability

Academic Institutions and Nonprofits only
pAAV_TALEN-FokI(WT)-BB_V3

Plasmid

#42588

Depositor

Feng Zhang

Article

Cong et al Nat Commun. 2012 Jul 24;3:968. doi: 10.1

Insert

TALEN-FokI(WT)-BB

Use

AAV; Adeno-associated virus ()

Tags

3XFlag

Expression

Mammalian

Promoter

CAG hybrid

Available Since

March 4, 2013

Availability

Academic Institutions and Nonprofits only

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pSW596 - mTurq2

Plasmid

#115985

Purpose

entry vector for GreenGate cloning method, CDS

Depositor

Jan Lohmann

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

mTurquoise2

Use

Unspecified

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pSB3K3-IC15

Plasmid

#165408

Purpose

Bacteria gene circuit on medium copy backbone.

Depositor

Xiaojun Tian

Article

Zhang et al Nat Commun. 2021 Feb 8;12(1):853. doi: 1

Insert

IC15 gene circuit

Expression

Bacterial

Available Since

June 4, 2021

Availability

Academic Institutions and Nonprofits only

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