We narrowed to 1,539 results for: MiCy

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pBBR15.2-2H12.D11opt

Plasmid

#221185

Purpose

cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter (Internal Lab ID: UJ11239)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Insert

cdGreen2.1

Expression

Bacterial

Promoter

Constitutive

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMRS-XylS-dCas9

Plasmid

#220196

Purpose

mini-Tn7 dCas9 expression vector - XylS/pM promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9

Use

CRISPR

Expression

Bacterial

Mutation

Pseudomonas aeruginosa codon optimized

Promoter

XylS/pM

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pLIB-HIS-TEV-scCAK1

Plasmid

#177014

Purpose

Generate baculovirus for insect cell expression of scCAK1 with N-terminal Polyhistidine-TEV

Depositor

Andrea Musacchio

Article

Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

Insert

CAK1 cyclin-dependent protein kinase-activating kinase CAK1 (CAK1 Budding Yeast)

Tags

Polyhistidine-TEV

Expression

Insect

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pMRE142

Plasmid

#118494

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sGFP2

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pGPA1

Plasmid

#115476

Purpose

Generation of mariner-based transposon mutant libraries in Enterococcus faecium

Depositor

Willem van Schaik

Article

Zhang et al BMC Genomics. 2017 Nov 21;18(1):893. doi

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)

Plasmid

#78231

Purpose

Expresses Cas9 protein, under the control of the TEF1 promoter, and CYC terminator. KanMX resistance

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

Cas9

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pACEBac1-PARP2-Flag-His-WT

Plasmid

#111574

Purpose

Expresses full length PARP2 in Sf9 (insect) cells

Depositor

Thomas Muir

Article

Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

Insert

PARP2 (PARP2 Human)

Tags

C-terminal 6xHis and C-terminal Flag

Expression

Insect

Promoter

polyhedrin promoter

Available Since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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pBSV2G_P0826-BB0323-mCherryBb

Plasmid

#118244

Purpose

Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes a BB0323-mCherry translational fusion under the control of the bb0826 promoter

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

BB0323-mCherry translational fusion under the control of the bb0826 promoter

Tags

mCherry

Expression

Bacterial

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pJMP1159

Plasmid

#119250

Purpose

rfp "test" strain

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA NT1/RR1 (rfp)

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCL1271

Plasmid

#217963

Purpose

Mammalian expression of Importin 5 (Imp5, IPO5) with an N-terminal eGFP fusion. Splice isoform 1.

Depositor

Andrew Bowman

Article

Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

Insert

Imp5 (IPO5 Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pACE-GIRK4-mC-S

Plasmid

#172427

Purpose

Expression of full-length, human GIRK4 with C-terminal mCherry StrepTag II

Depositor

Arthur Laganowsky

Article

Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

Insert

GIRK4 (KCNJ5 Human)

Tags

mCherry Streptag II

Expression

Insect

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pJMP1237

Plasmid

#119262

Purpose

for cloning new sgRNAs

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

dcas9

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pTn7CdrA-gfpC

Plasmid

#111616

Purpose

Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

Depositor

Tim Tolker-Nielsen

Article

Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

Insert

PcdrA-RBSII-gfp(Mut3)-T0-T1

Expression

Bacterial

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pFast-hHus1

Plasmid

#222469

Purpose

Expresses hHus1 in insect cells

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al Proc Natl Acad Sci U S A. 2001 Sep 25;98

Insert

hHus1 (HUS1 Human)

Expression

Insect

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCdrA-gfp(ASV)C

Plasmid

#111615

Purpose

Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

Depositor

Tim Tolker-Nielsen

Article

Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

Insert

PcdrA-RBSII-gfp(ASV)-T0-T1

Expression

Bacterial

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pTP288

Plasmid

#113460

Purpose

human Flag tagged Nbs1 in baculovirus transfer vector

Depositor

Tanya Paull

Article

Lee et al J Biol Chem. 2013 May 3;288(18):12840-51

Insert

Nbs1 (NBN Human)

Tags

Flag

Expression

Insect

Promoter

polyhedrin

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-PGAL1-3HA

Plasmid

#41608

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

3xHA, A. gossypii translation elongation factor 1…

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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SOX D221A/E244A/K246A pACEmam1

Plasmid

#192050

Purpose

Expresses a 3X catalytic mutant of SOX (ORF37)

Depositor

Britt Glaunsinger

Article

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

SOX (3X catalytic mutant)

Use

Unspecified

Mutation

3X catalytic mutant D221A/E244A/K246A

Promoter

CMV

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-Gm-Pc-mTurquoise2

Plasmid

#111259

Purpose

mini-Tn7 vector for insertion of mTurquoise2 (codon optimized for P. fluorescens) at bacterial attTn7 site

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

mTurquoise2

Expression

Bacterial

Mutation

mTurquoise2 is codon optimized for expression in …

Promoter

Pc

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE143

Plasmid

#118495

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sYFP2

Expression

Bacterial

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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