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Plasmid#175422PurposeExpresses R99A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.DepositorInsertCoV2 R99A nsp1
UseTags3XFLAG HaloExpressionMammalianMutationPromoterCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-Flex-Rev-3xGFP
Plasmid#122500PurposeCre dependent GFP expression.DepositorInsert3x GFP
UseAAVTagsExpressionMutationeach GFP has unique nt sequencePromoterAvailable sinceJan. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
NK697
Plasmid#120856PurposeReporter gene expression in S. rosettaDepositorInsertpac-P2A-mTFP-Jumblelw1
UseChoanoflagellate expressionTagsExpressionMutationJumblelw1 allele: T to C at nt 1109 causes L305P …PromoterAvailable sinceJan. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
NK695
Plasmid#120854PurposeReporter gene expression in S. rosettaDepositorInsertpac-P2A-Jumblelw1-mTFP
UseChoanoflagellate expressionTagsExpressionMutationJumblelw1 allele: T to C at nt 1109 causes L305P …PromoterAvailable sinceJan. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAF0084 Ef1a-Cp36-2a-EGFP
Plasmid#193462PurposeExpression of RecombinaseDepositorInsertCp36
UseTagsExpressionMammalianMutationPromoterEf1aAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
BPK1520
Plasmid#65777PurposeHuman expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNADepositorInsertSpCas9 gRNA backbone, without spacer sequence
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAF0033 Ef1a-Pa01-2a-EGFP
Plasmid#193460PurposeExpression of RecombinaseDepositorInsertPa01
UseTagsExpressionMammalianMutationPromoterEf1aAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAF0687 Pa01_attP-mCherry donor
Plasmid#193466PurposePromoterless mcherry donor for Pa01DepositorInsertPa01_attP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAF0036 Ef1a-Si74-2a-EGFP
Plasmid#193463PurposeExpression of RecombinaseDepositorInsertSi74
UseTagsExpressionMammalianMutationPromoterEf1aAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNTI647 dCas9-Mxi1 TetR KanMX
Plasmid#139474PurposeIntegrates CRISPRi effector and tet-responsive regulatorDepositorInsertsdCas9-Mxi1
Tet Repressor
UseCRISPRTagsMxi1 and NLSExpressionYeastMutationD10A, H840APromoterpGPM1 and pTEF1Available sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAF0034 Ef1a-Kp03-2a-EGFP
Plasmid#193461PurposeExpression of RecombinaseDepositorInsertKp03
UseTagsExpressionMammalianMutationPromoterEf1aAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
VP12
Plasmid#72247PurposeHuman expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAGDepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
UseCRISPRTags3x FLAG and NLSExpressionMammalianMutationN497A, R661A, Q695A and Q926APromoterCMVAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAF0035 Ef1a-Nm60-2a-EGFP
Plasmid#193464PurposeExpression of RecombinaseDepositorInsertNm60
UseTagsExpressionMammalianMutationPromoterEf1aAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAF0689 Ef1a-Pa01_attB-BFP acceptor
Plasmid#193472PurposeAcceptor plasmid for 3 plasmid recombination assayDepositorInsertPa01_attB
UseTagsExpressionMammalianMutationPromoterEf1aAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
BPK1179
Plasmid#179296PurposeExpresses dCas9 fused to four DmrA domainsDepositorInsertdCas9-DmrA(x4)
UseTagsExpressionMammalianMutationD10A, H840A (catalytically inactive Cas9)PromoterCAGAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAF0432 Ef1a-Dn29-2a-EGFP
Plasmid#193459PurposeExpression of Dn29 RecombinaseDepositorInsertDn29
UseTagsExpressionMammalianMutationPromoterEf1aAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAF0688 Kp03_attP-mCherry donor
Plasmid#193467PurposePromoterless mcherry donor for Kp03DepositorInsertKp03_attP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAF0451 Si74_attP-mCherry donor
Plasmid#193470PurposePromoterless mcherry donor for Si74DepositorInsertSi74_attP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJP-HBB-nLuc
Plasmid#175431PurposeExpresses nLuc with 5' leader of human beta globin (HBB) in mammalian cells.DepositorInsertnLUC plus 5' HBB leader
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAF0474 Ef1a-Si74_attB acceptor
Plasmid#193475PurposeAcceptor plasmid for 3 plasmid recombination assayDepositorInsertSi74_attB
UseTagsExpressionMammalianMutationPromoterEf1aAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only