We narrowed to 7,051 results for: Pet2;

1. pET28-At_PRORP1-His

   Plasmid

   #67869

   Purpose

   bacterial expression of PRORP1 (At2g32230), full coding sequence (mitoch./chloropl. form) + C-term. His tag

   Depositor

   Walter Rossmanith

   Article

   Pavlova et al Chembiochem. 2012 Oct 15;13(15):2270-6.

   Insert

   PRORP1 (PRORP1 Mustard Weed)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Residues 36-572 (see comments)

   Promoter

   T7

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-ClbN-NHis

   Plasmid

   #51497

   Purpose

   NRPS module containing CATE domains with N-terminal 6His tag

   Depositor

   Emily Balskus

   Article

   Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

   Insert

   ClbN
   

   Tags

   6x His tag and T7 tag

   Expression

   Bacterial

   Mutation

   contains aa30-1094 of NP_754347.1

   Promoter

   T7

   Available Since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-PTBP1-RRM12CCtoSS

   Plasmid

   #89155

   Purpose

   Recombinant expression of PTBP1-RRM12 C250S,C251S in E.coli

   Depositor

   Frédéric Allain

   Article

   Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

   Insert

   Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 1+2 mutant C250S, C251S (PTBP1 Human)
   

   Tags

   hexa His

   Expression

   Bacterial

   Mutation

   mutations: Cys 250 to Ser, Cys 251 to Ser

   Promoter

   T7

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28-p53 (1-73) (72R)

   Plasmid

   #62082

   Purpose

   Expression of human p53 (1-73) (72R) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

   Insert

   p53 (TP53 Human)
   

   Tags

   HIs

   Expression

   Bacterial

   Mutation

   contains TAD residues 1-73; 72R

   Promoter

   T7

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pET28a-Hook3 aa 1-239

   Plasmid

   #74609

   Purpose

   Expresses Hook domain and first coiled coil of Hook3 in bacteria for purification

   Depositor

   Ron Vale

   Article

   Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

   Insert

   human Hook3 amino acids 1-239 (HOOK3 Human)
   

   Tags

   6x His, Strep II, and superfolder GFP

   Expression

   Bacterial

   Available Since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28 mSmo-Cterm

   Plasmid

   #58703

   Purpose

   used for anti-mouse Smoothened antibody production (for affinity purification)

   Depositor

   Matthew Scott

   Article

   Rohatgi et al Science. 2007 Jul 20;317(5836):372-6.

   Insert

   Smo (Smo Mouse)
   

   Tags

   6X-HIS

   Expression

   Bacterial

   Mutation

   contains C terminus (aa 543-793)

   Available Since

   Sept. 12, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pET28b+R3(R112C/T180C)

   Plasmid

   #108195

   Purpose

   Expresses PdR-PCNA3(R112C/T180C) fusion protein in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

   Insert

   PdR-PCNA3(R112C/T180C) fusion protein
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   R112C/T180C(PCNA3)

   Promoter

   T7 promoter

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET29b-Ava3858-3857

   Plasmid

   #45744

   Depositor

   Christopher T Walsh, Emily Balskus

   Article

   Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

   Insert

   Ava3858-3857
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pET20b-delta133HtrA2 S400D

   Plasmid

   #16156

   Depositor

   L. Miguel Martins

   Article

   Plun-Favreau et al Nat Cell Biol. 2007 Nov;9(11):1243-52. d

   Insert

   HtrA2 (HTRA2 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   S400D

   Available Since

   Feb. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pET28b Me31B-3Myc

    Plasmid

    #113001

    Purpose

    For protein expression and purification of Myc-tagged full-length Drosophila me31B

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    full length me31B (me31B Fly)
    

    Tags

    Myc

    Expression

    Bacterial

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET20b-delta133HtrA2 G399S

    Plasmid

    #16158

    Depositor

    L. Miguel Martins

    Article

    Strauss et al Hum Mol Genet. 2005 Aug 1. 14(15):2099-1

    Insert

    HtrA2 (HTRA2 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    G399S

    Promoter

    T7

    Available Since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pET29NK-LGK/mGFPmut3

    Plasmid

    #81168

    Purpose

    GFP fusion to levoglucosan kinase

    Depositor

    Timothy Whitehead

    Article

    Wrenbeck et al ACS Synth Biol. 2019 Mar 15;8(3):474-481

    Insert

    Levoglucosan kinase wild-type
    

    Tags

    His6x and mGFPmut3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET20b-delta133HtrA2 S142A

    Plasmid

    #16153

    Depositor

    L. Miguel Martins

    Article

    Plun-Favreau et al Nat Cell Biol. 2007 Nov;9(11):1243-52. d

    Insert

    HtrA2 (HTRA2 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    S142A

    Available Since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pET21a-hAIM2-deltaPYD

    Plasmid

    #51522

    Purpose

    Express His6-tagged HIN domain of hAIM2 in bacteria

    Depositor

    Emad Alnemri

    Article

    Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

    Insert

    AIM2 (AIM2 Human)
    

    Tags

    His6 and T7

    Expression

    Bacterial

    Mutation

    deletion of pyrin domain

    Promoter

    lac operator

    Available Since

    March 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET22b-ecDHFR-G51PEKN

    Plasmid

    #118582

    Purpose

    Bacterial expression of E.coli DHFR G51PEKN mutant

    Depositor

    Stephen Benkovic

    Article

    Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

    Insert

    DHFR
    

    Expression

    Bacterial

    Mutation

    G51 mutated to P51; Glu-Lys-Asn residues inserted…

    Promoter

    T7

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28-At_PRORP3-His

    Plasmid

    #67871

    Purpose

    bacterial expression of PRORP3 (At4g21900), full coding sequence + C-term. His tag

    Depositor

    Walter Rossmanith

    Article

    Pavlova et al Chembiochem. 2012 Oct 15;13(15):2270-6.

    Insert

    PRORP3 (PRORP3 Mustard Weed)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28b-sad1-C63A

    Plasmid

    #90302

    Purpose

    protein expression

    Depositor

    Christine Guthrie

    Article

    Hadjivassiliou et al RNA. 2014 May;20(5):656-69. doi: 10.1261

    Insert

    SAD1
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    C63A

    Available Since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a His6Flag2AUF1(p40)

    Plasmid

    #135940

    Purpose

    Expresses AUF1p40 in E. coli

    Depositor

    Yimon Aye

    Article

    Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

    Insert

    heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
    

    Tags

    Flag2 and His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET22b-ecDHFR-Y100F

    Plasmid

    #110826

    Purpose

    Bacterial expression of E.coli DHFR Y100F mutant

    Depositor

    Stephen Benkovic

    Article

    Liu et al Proc Natl Acad Sci U S A. 2014 Dec 23;11

    Insert

    ecDHFR
    

    Expression

    Bacterial

    Mutation

    Y100 mutated to F100

    Promoter

    T7

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET20b-delta133HtrA2 S142D

    Plasmid

    #16154

    Depositor

    L. Miguel Martins

    Article

    Plun-Favreau et al Nat Cell Biol. 2007 Nov;9(11):1243-52. d

    Insert

    HtrA2 (HTRA2 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    S142D

    Available Since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only