We narrowed to 996 results for: trna

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GLN, Linear-3, PolyU(4)

Plasmid

#166985

Purpose

Tests for the impact of 4 uracil in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination module:PolyU(4)

Expression

Mammalian

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pAcBac1-EcTrp-TGA

Plasmid

#228780

Purpose

aaRS/tRNA system for incorporation of 5HTP within proteins in mammalian cells at TGA codon

Depositor

Abhishek Chatterjee

Article

Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

Inserts

EcTrpRS
EcWtR

Use

Baculoviral

Expression

Mammalian

Mutation

TGA suppressor

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pOPO646

Plasmid

#195307

Purpose

pBAD322-MycaTnsD, arapBAD promoter expressing cyanobacterial tRNA-Leu gene targeting TnsD protein of McCAST (Tn7575).

Depositor

Joseph Peters

Article

Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

Insert

TnsD of McCAST

Expression

Bacterial

Promoter

Arabinose inducible arapBAD

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pTAG4E

Plasmid

#176868

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_4E

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTAG5

Plasmid

#176869

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_5

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTAG6E

Plasmid

#176870

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_6E

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTAG2

Plasmid

#176864

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_2

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTAG3

Plasmid

#176866

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_3

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTAG4

Plasmid

#176867

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_4

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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GLN, Linear-3, PolyU(1), Hairpin Distance = 0nt

Plasmid

#166986

Purpose

Tests for the impact of 1 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination module:PolyU(1), Hairpin = 0 nt

Expression

Mammalian

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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GLY, Linear-3, PolyU(4), Hairpin Distance = 0nt

Plasmid

#166991

Purpose

Tests for the impact of 4 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLY tRNA promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination module:PolyU(4), Hairpin = 0 nt

Expression

Mammalian

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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GLY, Linear-3, PolyU(1), Hairpin Distance = 0nt

Plasmid

#166990

Purpose

Tests for the impact of 1 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLY tRNA promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination module:PolyU(1), Hairpin = 0 nt

Expression

Mammalian

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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GLN, Linear-3, PolyU(4), Hairpin Distance = 0nt

Plasmid

#166987

Purpose

Tests for the impact of 4 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination module:PolyU(4), Hairpin = 0 nt

Expression

Mammalian

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pRS423-ProSgH

Plasmid

#163974

Purpose

Helper plasmid for cloning gRNAs under the control of the tRNA(Pro) promoter

Depositor

Jiazhang Lian

Article

Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pWR63(YUM1)

Plasmid

#203336

Purpose

episomal Cas9 and YUM1-targeting sgRNA expression vector for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
CCW12 promoter (S. stipitis)
CaCas9
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1
THD3 promoter (S. stipitis)
tRNA-sgRNA(SapI)-HDV
ADH1 terminator (S. stipitis)
YUM1-targeting sequence

Use

CRISPR

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAcBac1-EcTyr-TAG

Plasmid

#228782

Purpose

EcTyr aaRS/tRNA system for mammalian ncAA incorporation

Depositor

Abhishek Chatterjee

Article

Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

Inserts

EcTyrRS
EcYtR
BstR

Use

Baculoviral

Expression

Mammalian

Mutation

TAG suppressor

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pAS_4xMmaPylT(UUA)_EF1_sfGFP150TAA

Plasmid

#174897

Purpose

ochre suppression reporter sfGFP150TAA expression, with MmaPylT(UUA) ochre suppressor tRNA cassette

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sf GFP

Expression

Mammalian

Mutation

150TAA in sfGFP

Promoter

EF1

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAS_4xMmaPylT(UCA)_EF1_sfGFP150TGA

Plasmid

#174898

Purpose

opal suppression reporter sfGFP150TGA expression, with MmaPylT(UCA) opal suppressor tRNA cassette

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sfGFP

Expression

Mammalian

Mutation

150TGA in sfGFP

Promoter

EF1

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGEL052

Plasmid

#137906

Purpose

OsBiP1 promoter driven Cas9-tRNA system

Depositor

Yong Zhang

Article

Ren et al Front Plant Sci. 2019 Sep 20;10:1173. do

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pGEL078

Plasmid

#137887

Purpose

iSTU-CRISPR/Cas9 with StIV2 intron, tRNA structure backbone

Depositor

Yong Zhang

Article

Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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