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pBMN YFP-ltDHFR(DD)

Plasmid

#47080

Purpose

ecDHFR based destable domain function at low temperature for regulating protein stability in Caenorhabditis elegans

Depositor

Thomas Wandless

Article

Cho et al PLoS One. 2013 Aug 22;8(8):e72393. doi:

Insert

YFP-ltDHFR(DD)

Use

Retroviral

Tags

HA and YFP

Mutation

amino acids mutations: R12H, N37D, N59D, G67S, D1…

Promoter

LTR

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

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ptdTomato-N1-FLCN

Plasmid

#49174

Purpose

Mammalian expression of mouse folliculin (FLCN) fused to tdTomato

Depositor

Shawn Ferguson

Article

Petit et al J Cell Biol. 2013 Sep 30;202(7):1107-112

Insert

Folliculin (Flcn Mouse)

Tags

tdTomato

Expression

Mammalian

Mutation

Stop codon deleted

Promoter

CMV

Available Since

Nov. 26, 2013

Availability

Academic Institutions and Nonprofits only

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pET27b-ecDHFR-N23PP/L28F

Plasmid

#118584

Purpose

Bacterial expression of E.coli DHFR N23PP and L28F mutant

Depositor

Stephen Benkovic

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

Insert

DHFR

Expression

Bacterial

Mutation

L28 mutated to F28, N23 mutated to P23, a proline…

Promoter

T7

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-FNIP1

Plasmid

#49175

Purpose

Mammalian expression of mouse folliculin interacting protein 1 (FNIP1) fused to GFP

Depositor

Shawn Ferguson

Article

Petit et al J Cell Biol. 2013 Sep 30;202(7):1107-112

Insert

Folliculin Interacting Protein 1 (Fnip1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

Stop codon deleted

Promoter

CMV

Available Since

Dec. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP DNAJB4

Plasmid

#19499

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJB4 (DNAJB4 Human)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pGEX-6P1-Ataxin3Q22

Plasmid

#22117

Depositor

Henry Paulson

Article

Winborn et al J Biol Chem. 2008 Sep 26. 283(39):26436-

Insert

Ataxin 3 (ATXN3 Human)

Tags

GST

Expression

Bacterial

Available Since

Nov. 17, 2009

Availability

Academic Institutions and Nonprofits only

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MSCV Myc P57S IRES GFP

Plasmid

#18771

Depositor

Scott Lowe

Article

Hemann et al Nature. 2005 Aug 11. 436(7052):807-11.

Insert

myc P57S (MYC Human)

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Mutation

P57S

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
pMyc4ElbLuc.

Plasmid

#53246

Purpose

reporter plasmid containing 4 myc cDNA binding sites

Depositor

Roger Davis

Article

Gupta et al Proc Natl Acad Sci U S A. 1993 Apr 15;90

Insert

pMyc4ElbLuc (MYC Human)

Mutation

To prepare plasmids with one, two, three, and fou…

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pET23-PBD(65-150)-N-Cys

Plasmid

#13722

Depositor

Klaus Hahn

Article

Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

Insert

PBD(65-150)-N-Cys (PAK1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Met-Cys-Gly-PBD(65-150)-

Available Since

March 28, 2007

Availability

Academic Institutions and Nonprofits only
pEYFP-hDF-C2A

Plasmid

#72582

Purpose

human dysferlin C2A domain tagged with YFP

Depositor

Renzhi Han

Article

Xu et al PLoS One. 2011;6(11):e27884. doi: 10.137

Insert

dysferlin C2A domain (DYSF Human)

Tags

EYFP

Expression

Mammalian

Mutation

C2A domain

Promoter

CMV

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag OSM

Plasmid

#12191

Depositor

Gary Johnson

Article

Uhlik et al Nat Cell Biol. 2003 Dec . 5(12):1104-10.

Insert

OSM (Ccm2 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+) Laccase2 MCS-ciRS7 Exon

Plasmid

#69900

Purpose

Expresses the human ciRS7/CDR1as circular RNA in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ciRS7 (stw, CDR1-AS Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pSR357.10

Plasmid

#125106

Purpose

Expression of narL(1-131aa)-ydfI(126-213aa) chimera under PLtetO-1, output Promoter PydfJ115

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

narL(REC)-ydfI(DBD)131
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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YFP-MEKK3

Plasmid

#12189

Depositor

Gary Johnson

Article

Uhlik et al Nat Cell Biol. 2003 Dec . 5(12):1104-10.

Insert

MEKK3 (Map3k3 Mouse)

Tags

EYFP

Expression

Mammalian

Available Since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
pEBB COMMD2 Flag

Plasmid

#74894

Purpose

Express COMMD2 in mammalian cells

Depositor

Ezra Burstein, Colin Duckett

Article

Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

Insert

COMMD2 (COMMD2 Human)

Tags

Flag

Expression

Mammalian

Promoter

EF-1alpha promoter

Available Since

June 23, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO V5 DNAJB4

Plasmid

#19526

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DnaJ (Hsp40) homolog, subfamily B, member 4 (DNAJB4 Human)

Tags

V5

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pENTR-ATXN1

Plasmid

#16133

Depositor

Huda Zoghbi

Article

Lim et al Cell. 2006 May 19. 125(4):801-14.

Insert

Ataxin1 (ATXN1 Human)

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
GST-Bcr pLEF

Plasmid

#38160

Depositor

Nora Heisterkamp

Article

Mishra et al Oncogene. 2003 Nov 13;22(51):8255-62.

Insert

Bcr (BCR Human)

Tags

GST

Expression

Mammalian

Promoter

SV40

Available Since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only
pCfB3051(gRNA X-3 XI-2 XII-2)

Plasmid

#73293

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-3, XI-2, and XII-2

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3-human C-MYC-V394D

Plasmid

#74162

Purpose

Mammalian expression of human cMyc VD mutant

Depositor

Martine Roussel

Article

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

cMyc (MYC Human)

Expression

Mammalian

Mutation

V394D

Promoter

CMV

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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