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pMXs-hcMYC (Plath)

Plasmid

#17966

Depositor

Kathrin Plath

Article

Lowry et al Proc Natl Acad Sci U S A. 2008 Feb 26. 1

Insert

v-myc myelocytomatosis viral oncogene homolog (avian) (MYC Human)

Use

Retroviral

Expression

Mammalian

Mutation

n/a

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only

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CABE-T1.2

Plasmid

#193258

Purpose

Expression of CABE-T1.2 base editor in mammalian cells, CMV promoter

Depositor

Nicole Gaudelli

Article

Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Insert

CABE-T1.2

Expression

Mammalian

Mutation

D10A (Cas9), TadA mutations within

Promoter

CMV

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [C12orf35]

Plasmid

#227245

Purpose

All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in CHO cell

Depositor

Jae Seong Lee

Article

Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.

Inserts

Tet-On 3G transactivator protein
Enhanced green fluorescent protein

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Promoter

EF1α and pTRE3G

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCry2PHR-mCh-Rac1

Plasmid

#42954

Purpose

Expresses optogenetically controlled Rac1

Depositor

David Schaffer

Article

Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

Insert

Cry2PHR-mCherry-Rac1 (RAC1 Human, Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

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BE3-Gam

Plasmid

#100807

Purpose

Expresses BE3-Gam in mammalian cells

Depositor

David Liu

Article

Komor et al Sci Adv. 2017 Aug 30;3(8):eaao4774. doi:

Insert

BE3-Gam

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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3341 pcDNA3 Bcl-2 T69A S70A S87A

Plasmid

#13340

Depositor

Stanley Korsmeyer

Article

Yamamoto et al Mol Cell Biol 1999 Dec;19(12):8469-78.

Insert

Bcl-2 (BCL2 Human)

Expression

Mammalian

Mutation

changed Threonine 69 to Alanine; changed Serine 7…

Available Since

Nov. 9, 2006

Availability

Academic Institutions and Nonprofits only
RRL.sin.cPPT.CMV/Flag-E2-crimson.IRES-puro.WPRE (MT06)

Plasmid

#139448

Purpose

Lentiviral vector to ectopically express Flag-tagged E2-crimson from CMV promoter

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

FLAG-tag E2-crimson

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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3xFLAG-dCas9/pMXs-IG

Plasmid

#51258

Purpose

Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

Depositor

Hodaka Fujii

Article

Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

Insert

3xFLAG-dCas9

Use

CRISPR and Retroviral

Tags

3xFLAG tag and NLS (nuclear localization signal)

Expression

Mammalian

Mutation

human codon-optimized, D10A + H840A

Promoter

LTR

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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Lmx1b

Plasmid

#34997

Depositor

Malin Parmar

Article

Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

Insert

LIM homeobox transcription factor 1-beta (Lmx1b Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

March 6, 2012

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-HBXIP

Plasmid

#42328

Depositor

David Sabatini

Article

Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

Insert

HBXIP (LAMTOR5 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV, SP6

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only
pL-CRISPR.SFFV.PAC

Plasmid

#57829

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, SFFV Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
SFFV
P2A-PAC

Use

CRISPR and Lentiviral

Tags

FLAG

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-STChRger2-TS-EYFP

Plasmid

#129393

Purpose

Soma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.

Depositor

Viviana Gradinaru

Article

Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

soma targeted ChRger2

Use

AAV

Tags

Kv2.1-TS-EYFP

Expression

Mammalian

Promoter

CaMKIIa

Available Since

Nov. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAG70 BDNF 3'UTR

Plasmid

#12021

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

BDNF 3'UTR (BDNF Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

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FLAG-ARP8

Plasmid

#15361

Depositor

Joan Conaway, Ronald Conaway

Article

Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

Insert

actin-related protein 8 (ACTR8 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only

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pMRXIP Lamp1-Venus

Plasmid

#89937

Purpose

Expresses Lamp1 tagged with Venus in mammalian cells

Depositor

Noboru Mizushima

Article

Tsuboyama et al Science. 2016 Nov 25;354(6315):1036-1041

Insert

rat Lamp1 (Lamp1 Rat)

Use

Retroviral

Tags

Venus

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pJ3 SHP2 D61A

Plasmid

#8320

Depositor

Ben Neel

Article

Bennett et al Mol Cell Biol 1996 Mar;16(3):1189-202.

Insert

SHP2 (PTPN11 Human)

Expression

Mammalian

Mutation

D61A (activated mutant)

Available Since

June 17, 2005

Availability

Academic Institutions and Nonprofits only
pSNV2 CHER NLS cop1at(335-675)

Plasmid

#44972

Depositor

Thanos Halazonetis

Article

Crefcoeur et al Nat Commun. 2013;4:1779. doi: 10.1038/nc

Insert

mCherry-NLS-COP1 (COP1 Mustard Weed)

Tags

NLS and mCherry

Expression

Mammalian

Mutation

deleted amino acids 1-334

Promoter

pCMV IE

Available Since

June 6, 2013

Availability

Academic Institutions and Nonprofits only

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pLX317-hcRED

Plasmid

#115440

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

hcRED

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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SPLINTR mCHERRY v1 backbone

Plasmid

#179778

Purpose

SPLINTR backbone vector

Depositor

Mark Dawson

Article

Fennell et al Nature. 2022 Jan;601(7891):125-131. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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Cas12f-GE ver4.0

Plasmid

#176543

Purpose

Expresses Cas12f-GE in mammalian cells

Depositor

Yong-Sam Kim

Article

Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

Inserts

Cas12f-GE ver4.0
Cas12f-GE ver4.0

Expression

Mammalian

Promoter

U6 and chicken β-actin

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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