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We narrowed to 13,357 results for: BASE

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Showing: 9421 - 9440 of 13357 results

  2. pLenti-nSpCas9-BEACON2

    Plasmid
    #209041
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where BEACON2 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    BEACON2-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209041-plasmid-map-sequence-id-419488

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  3. pTRIP TRE BI_SEPT9_i1-14-GFP11_SEPT9_i1-14-GFP10

    Plasmid
    #180351
    Purpose
    mammalian co-expression of human SEPT9_i1 fused to GFP10 and of human SEPT9_i1 fused to GFP11
    Depositor
    Manos Mavrakis
    Article
    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301
    Inserts
    SEPTIN9_v1 (SEPTIN9 Human)
    SEPTIN9_v1 (SEPTIN9 Human)
    IRES-TagBFP
    Use
    Lentiviral
    Tags
    GFP10 and GFP11
    Expression
    Mammalian
    Promoter
    bidirectional TRE
    Available Since
    Dec. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    180351-plasmid-map-sequence-id-357912

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  4. pTRIP TRE BI_SEPT2 NCmut-14-GFP11_SEPT2 NCmut-14-GFP10

    Plasmid
    #180357
    Purpose
    mammalian co-expression of human SEPT2 NCmut fused to GFP10 and of human SEPT2 NCmut fused to GFP11
    Depositor
    Manos Mavrakis
    Article
    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301
    Inserts
    SEPTIN2 (SEPTIN2 Human)
    SEPTIN 2 (SEPTIN2 Human)
    IRES-TagBFP
    Use
    Lentiviral
    Tags
    GFP10 and GFP11
    Expression
    Mammalian
    Mutation
    SEPT2 F20D, V27D
    Promoter
    bidirectional TRE
    Available Since
    Dec. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    180357-plasmid-map-sequence-id-358018

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  5. pTRIP TRE BI_SEPT9_i1 NCmut-14-GFP11_SEPT9_i1 NCmut-14-GFP10

    Plasmid
    #180358
    Purpose
    mammalian co-expression of human SEPT9_i1 NCmut fused to GFP10 and of human SEPT9_i1 NCmut fused to GFP11
    Depositor
    Manos Mavrakis
    Article
    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301
    Inserts
    SEPTIN9_v1 (SEPTIN9 Human)
    SEPTIN9_v1 (SEPTIN9 Human)
    IRES-TagBFP
    Use
    Lentiviral
    Tags
    GFP10 and GFP11
    Expression
    Mammalian
    Mutation
    SEPT9_i1 I281D, M288D
    Promoter
    bidirectional TRE
    Available Since
    Dec. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    180358-plasmid-map-sequence-id-358016

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  15. pCfB2375

    Plasmid
    #67546
    Purpose
    EasyClone system-based yeast integrative vector carrying loxP-flanked natMX marker, integration into S. cerevisiae XI-1 chromosomal location, USER site for cloning, amp resistance
    Depositor
    Irina Borodina
    Article
    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.
    Type
    Empty backbone
    Use
    Cre/Lox; Integrative
    Expression
    Yeast
    Available Since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    67546-plasmid-map-sequence-id-116391

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  16. pYPQ-ABE-SpRYn-Act3.0

    Plasmid
    #178959
    Purpose
    It consists of a SpRY nickase (D10A) fused with an adenine deaminase (ABE8e) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous A to G conversion and gene activation.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:
    Insert
    ABE8e-SpRY-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available Since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    178959-plasmid-map-sequence-id-354322

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  17. LSB-hsa-miR-21-5p

    Plasmid
    #103345
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-21-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-21-5p target (MIR21 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103345-plasmid-map-sequence-id-195739

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  18. A3Bi-ctd-Cas9n-UGI-NLS

    Plasmid
    #109426
    Purpose
    Expresses the C-terminal catalytic domain of human APOBEC3B containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.
    Depositor
    Reuben Harris
    Article
    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994
    Insert
    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Insertion of an L1 intron into the coding sequenc…
    Promoter
    CMV
    Available Since
    July 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    109426-plasmid-map-sequence-id-212924

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  19. pAAV-synmiR-L-mRuby-Pro-EGFP-4×19

    Plasmid
    #218264
    Purpose
    Expresses EGFP in mammalian cells regulated by a synthetic microRNA-based dosage compensation circuit
    Depositor
    Michael Elowitz
    Insert
    synPolyA-synmiRL-mRuby-EF1a-CMVe_MeP-EGFP-MREL_4×19
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Available Since
    May 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    218264-plasmid-map-sequence-id-509434

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  20. pAAV-synmiR-7-mRuby-Pro-EGFP-8×12

    Plasmid
    #218253
    Purpose
    Expresses EGFP in mammalian cells regulated by a synthetic microRNA-based dosage compensation circuit
    Depositor
    Michael Elowitz
    Insert
    synPolyA-synmiR7-mRuby-EF1a-CMVe_MeP-EGFP-MRE7_8×12
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Available Since
    May 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    218253-plasmid-map-sequence-id-509430

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Showing: 9421 - 9440 of 13357 results