We narrowed to 23,538 results for: Sis;

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pDGE661

Plasmid

#153259

Purpose

M5 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-MKRN1 H307E

Plasmid

#78749

Purpose

To overexpress MKRN1 H307E in Mammalian Cell

Depositor

Jaewhan Song

Article

Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

Insert

MKRN1 (MKRN1 Human)

Tags

HA

Expression

Mammalian

Mutation

H307E

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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KG#470

Plasmid

#110930

Purpose

Expresses proteins pan-neuronally in C. elegans (entire nervous system)

Depositor

Kenneth Miller

Article

Schade et al Genetics. 2005 Feb;169(2):631-49. doi: 1

Inserts

rab-3 promoter
unc-54 3' control region

Expression

Bacterial

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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Osa-pCAMBIA-1301-STTM394

Plasmid

#84204

Purpose

Disturb the expression of miR394 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR394

Expression

Bacterial, Plant, and Yeast…

Promoter

2x35S

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pET151-mUCH37 (ISF2)

Plasmid

#61928

Purpose

Expression of mUCH37 (ISF2) in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Insert

UCH37 (M.musculus) (Uchl5 Mouse)

Tags

6x HIS

Expression

Bacterial

Promoter

T7

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pGL3-PIM2

Plasmid

#51848

Purpose

promoter region (-550;0) of PIM2

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

PIM2 (PIM2 Human)

Use

Luciferase; Reporter vector

Available Since

July 17, 2014

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-11

Plasmid

#160711

Purpose

For C-terminal tags with Trichoderma optimised Synthetic eGFP bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

Trichoderma optimised Synthetic eGFP

Use

Synthetic Biology

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pRibo-Tt

Plasmid

#154124

Purpose

Expresses wt Ribo-T rRNA and a set of tRNAs in bacterial cells

Depositor

Alexander Mankin

Article

Aleksashin et al Nat Commun. 2020 Apr 20;11(1):1858. doi:

Inserts

engineered bacterial rRNAs operon under control of pL promotor
bacterial tRNAs (Asp, Trp, Ile, Ala, Glu) operon under control of Ptac promoter

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptac and pL

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pRAN334

Plasmid

#120814

Purpose

E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt)

Depositor

Craig Ellermeier, David S. Weiss

Article

Ransom et al J Bacteriol. 2014 Jun;196(12):2290-300.

Insert

cyan fluorescent protein, codon-optimized for low GC organisms

Expression

Bacterial

Promoter

Ptet

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pET-CV-VAMP2

Plasmid

#168023

Purpose

Expresses eCFP-VAMP2(T27-R86)-mVenus in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

eCFP-VAMP2(T27-R86)-mVenus

Tags

Hisx6

Expression

Bacterial

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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SEPT5 Mut(S327A)

Plasmid

#27274

Depositor

Harish Pant

Article

Amin et al J Neurosci. 2008 Apr 2. 28(14):3631-43.

Insert

Septin 5 (SEPTIN5 Human)

Tags

Myc (MASMQKLISEEDL)

Expression

Mammalian

Mutation

S327A

Available Since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only
Bov.hsc70.T77.A1-386.wt

Plasmid

#12534

Depositor

David McKay

Article

Wilbanks et al J Biol Chem. 1994 Apr 29. 269(17):12893-

Insert

Bovine 70 kDa heat shock protein ATPase fragment

Expression

Bacterial

Mutation

deletion of residues 387-650 of bovine Hsc70 prot…

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

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G273D HA-dynamin 1 pUHD10-3

Plasmid

#34689

Depositor

Sandra Schmid

Article

Damke et al J Cell Biol. 1995 Oct;131(1):69-80.

Insert

dynamin 1 (DNM1 Human)

Tags

HA (variant)

Expression

Mammalian

Mutation

G273D

Available Since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only
pCA-ATG-intron-tdT3Myc

Plasmid

#36883

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Insert

tdTomato-3Myc

Tags

3 Myc tags

Expression

Mammalian

Mutation

beta-globin intron containing a loxP site inserte…

Promoter

CAG (chicken beta actin promoter and CMV enhancer)

Available Since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

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HisSumoOphP

Plasmid

#189672

Purpose

HisSUMO tagged OphP to express OphP in pichia pastorias.

Depositor

Markus Künzler, James Naismith

Article

Emmanuel et al bioRxiv 2022.07.21.500988

Insert

HisSumoOphP

Tags

HisSumo tagged

Expression

Yeast

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAGM1761

Plasmid

#105381

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

promoter AtDMC1 (At3G22880; 3000bp)

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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NET39 mRFP-C1 (1219)

Plasmid

#62042

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

NET39 (PPAPDC3 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only
pUC_h7SK Mma PylT (CUA)

Plasmid

#154782

Purpose

plasmid with single copy of Mma PylT (CUA anticodon)

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

Mma PylT

Expression

Mammalian

Mutation

U25C

Promoter

h7SK

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z14

Plasmid

#120057

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mNeptune2.5

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mNeptune2.5

Use

Pcr template

Tags

mNeptune2.5

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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Psicheck2 TNC full length 3'UTR

Plasmid

#26995

Depositor

Joan Massague

Article

Tavazoie et al Nature. 2008 Jan 10. 451(7175):147-52.

Insert

TNC Full length 3' UTR (TNC Human)

Use

Luciferase

Tags

Renilla Luciferase

Expression

Mammalian

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only