We narrowed to 15,448 results for: NTS

Showing: 9441 - 9460 of 15448 results

Results per page:

Sort results by:

Lenti-pNFkB::Cre-mRuby3

Plasmid

#164137

Purpose

Lentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of TNFa

Depositor

Hyongbum Kim

Article

Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

Insert

NFkB promoter-Cre-T2A-mRuby3

Use

CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…

Expression

Mammalian

Promoter

NFkB synthetic promoter

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.CAG-FLEX.SF-iGluSnFR.A184V.mRuby3

Plasmid

#106205

Purpose

Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184V

Use

AAV

Mutation

GltI: A184V + mRuby3 fusion

Promoter

CAG

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST17-cDAXX

Plasmid

#169729

Purpose

Bacterial expression of N-terminally 6His tagged cDAXX

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

DAXX (DAXX Human)

Tags

6 His

Expression

Bacterial

Promoter

T7

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pX62

Plasmid

#46854

Purpose

TOP Hupki-puro2A-G245S plasmid to introduce mutant p53 to PLF ESC or MEFs by IMCE

Depositor

Monica Hollstein

Article

Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

Insert

attB-Puro-2A and Hupki p53 G245S (TP53 Human, Mouse)

Expression

Mammalian

Mutation

G245S

Available Since

Aug. 22, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pX68

Plasmid

#46857

Purpose

OP Hupki-puro2A-A138V plasmid to indroduce mutant p53 to PLF ESC or MEFs by IMCE

Depositor

Monica Hollstein

Article

Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

Insert

attB-Puro-2A and Hupki p53 A138V (TP53 Human, Mouse)

Expression

Mammalian

Mutation

p53 A138V

Available Since

Aug. 22, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-MTS-KD-c-Src-FLAG

Plasmid

#44653

Depositor

Yoshimi Homma

Article

Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

Insert

v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)

Tags

FLAG tag and mitochondria-targeting sequence (MTS…

Expression

Mammalian

Mutation

changed lysine 298 to methionine

Promoter

CMV promoter

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
MAC-C Sars-CoV-2 NSP12_P314L

Plasmid

#194813

Purpose

Sars-CoV-2 NSP12_P314L in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

NSP12_P314L (ORF1ab Sars-CoV-2)

Tags

MAC-C

Expression

Mammalian

Mutation

P314L

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pTwist-SARS-CoV-2 Δ18 B.1.351v3

Plasmid

#169464

Purpose

Encodes SARS-CoV-2 B.1.351v3 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 B.1.351v3 Spike truncated to remove 18 amino acids (S )

Expression

Mammalian

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
SPDK3889(TRV-RNA2-pPEBV-MCS-AtMet-tRNA)

Plasmid

#149277

Purpose

Modified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAs

Depositor

Savithramma Dinesh-Kumar

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

Modified TRV RNA2 with PEBV promoter and AtMet-tRNA mobile RNA sequence

Use

T-dna vector

Mutation

T2318C (DNA) in PEBV coat protein sequence

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)_Granulin 4-no linker

Plasmid

#176925

Purpose

express Granulin 4 without linker 5 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Granulin 4 (GRN Human)

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1_mGFP-FGFR3-K508M

Plasmid

#191753

Purpose

Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-K508M leads to inactive receptor (target mutation: c.1523A>T; silent mutation added on purpose: c.1515A>T)

Depositor

Irene Tiemann-Boege

Article

Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

Insert

mGFP-FGFR3IIIc

Tags

mGFP

Expression

Mammalian

Mutation

c.1523A>T (p.K508M) + silent mutation c.1515A&…

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
eSpCas9-plus

Plasmid

#126767

Purpose

Expression plasmid for human codon-optimized increased fidelity eSpCas9-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

eSpCas9-plus

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

K848A, R1060A; amino acids 1005-1013 replaced wit…

Promoter

CBh

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
px330-GAPDH

Plasmid

#136940

Purpose

NHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human GAPDH; induce CD4+ deletion rearrangement by pairing w/ px330-CD4

Depositor

Jeremy Stark

Article

Bhargava et al J Biol Chem. 2019 Nov 21. pii: RA119.010

Insert

sgRNA targeting GAPDH (GAPDH Human)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
1_T7-mScarlet3-Hras

Plasmid

#225928

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the H-Ras membrane localisation signal

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv [Preprint]. 2025 Nov 29:2024.11.

Insert

mScarlet3-HRas

Use

In vitro transcription

Promoter

T7

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGB3 alpha2 P35S:MS2:VPR:Tnos (GB1830)

Plasmid

#160624

Purpose

TU for the constitutive expression of the MS2 coat protein fused on Ct to the activation domain VPR (VP64+p65+Rta)

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

P35S:MS2:VPR:Tnos

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLB052 - deAct - SpVB

Plasmid

#136286

Purpose

entry vector for GreenGate cloning method, D module, open reading frame of the mono(ADP-ribosyl) transferase domain of the Salmonella enterica SpvB

Depositor

Alexis Maizel

Article

Vilches Barro et al Curr Biol. 2019 Aug 5;29(15):2443-2454.e

Insert

deAct - SpvB

Expression

Bacterial

Available Since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Venus-Puma-pEGFP-C1

Plasmid

#166739

Purpose

Express Venus fused to the N-terminus of the Bcl-2 family protein, Puma

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

Puma (BBC3 Human)

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 LIC 6A HIS-TEV-DPP9S

Plasmid

#166791

Purpose

Mammalian cell expression vector for His-tagged DPP9S

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Tags

HIS-TEV

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
2_T7-mScarlet3-KRas

Plasmid

#225929

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the K-Ras membrane localisation signal

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv [Preprint]. 2025 Nov 29:2024.11.

Insert

mScarlet3-KRas

Use

In vitro transcription

Promoter

T7

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.hSynap-FLEX.SF-iGluSnFR.A184V

Plasmid

#106181

Purpose

Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Has Service

AAV1

Insert

SF-iGluSnFR.A184V

Use

AAV

Mutation

GltI: A184V

Promoter

hSynapsin

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge