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Plasmid#195740PurposeExpress APOVEC1-nCas9(D10A)-UGI using phenol inducible system in Methanotroph or E. coliDepositorPublicationInsertDi-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterPo (phoenol-inducible promoter)Available sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-SQSTM1-MUT
Plasmid#194583PurposeMammalian Expression of mEGFP-SQSTM1 Mutant (V271Sfs*41) Fusion ProteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSQSTM1 (SQSTM1 Human)
TagsmEGFPExpressionMammalianMutationV271Sfs*41Available sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CoV2-Omicron BA.1 spike
Plasmid#194602PurposeMammalian expression of RBD domain from SARS-CoV-2 omicron (BA.1)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCoV2-Omicron BA.1 spike (S Severe acute respiratory syndrome coronavirus 2)
ExpressionMammalianAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-EFS-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP
Plasmid#188771PurposedCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)DepositorInsertdCas9-XTEN80-KRAB(Kox1)
UseLentiviralTagsHA-2xNLS-XTEN80(linker)-KRAB(Kox1)PromoterEFSAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-IpgD(C439S)
Plasmid#183667PurposeN-terminally tagged Shigella flexneri IpgD (catalytically inactive) for mammalian expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertIpgD
TagsEGFPExpressionMammalianMutationC439SPromoterCMVAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-nl.EGFP-FlpO
Plasmid#172379PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertnuclear-localized EGFP + FlpO
UseNeurotropic virusAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmRuby2-CrowdingLinker-Clover
Plasmid#171058PurposeExpresses tandem mClover2-mRuby2 FRET pair with a crowdedness-sensitve linker domain to sense cytosolic crowdedness.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCrowdedness-sensitive linker domain
TagsmClover2 and mRuby2ExpressionMammalianPromoterCMVAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS20: pHelper(PmcCAST)_PmcPSP1_ΔTnsD
Plasmid#168153PurposeInducible expression of PmcCAST proteins (except TnsD) with crRNA targeting PmcPSP1.DepositorInsertsPmcCAST minimal CRISPR array (with spacer for PmcPSP1)
PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
PmcCAST Tns proteins (TnsAB and TnsC)
PmcTniQ
ExpressionBacterialAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS18: pHelper(PmcCAST)_PmcPSP1
Plasmid#168151PurposeInducible expression of PmcCAST proteins with crRNA targeting PmcPSP1.DepositorInsertsPmcCAST minimal CRISPR array (with spacer for PmcPSP1)
PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
PmcCAST Tns proteins (TnsAB, TnsC and TnsD)
PmcTniQ
ExpressionBacterialAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCS2-mCherry-MKLP1
Plasmid#140562PurposeExpresses mCherry tagged MKLP1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMKLP1 (KIF23 Human)
Tags2xmCherryExpressionMammalianPromoterCMV IE94Available sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8611 PB-Tre3g-Fluc-DR-crIFNg-DR-crIFNg-DR-SV40PolyA/ R(EF1a- Zeo–t2A-rtTA-WPRE)
Plasmid#140226PurposeDox inducible expression of luciferase + crIFNg. Constitutive EF1a driven rtTA and zeocin resistanceDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLuciferase + crIFNg
ExpressionMammalianPromoterTRE3GAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
p-dCas9-HDAC1-H141A-Hygro
Plasmid#104417Purposetransient expression of dCas9-dHDAC1 fusion proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdHDAC1 (HDAC1 Human)
UseCRISPRExpressionMammalianMutationH141APromoterCMV promoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
H513 px330.sgActin.UTR.2
Plasmid#138176PurposeCRISPR SONIC: Encodes codon-optimized SpCas9 and gRNA for targeting 3' UTR of β-actin.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsgActin (Actb Mouse)
UseCRISPRExpressionMammalianPromoterU6Available sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBV-luc TOP2A 1kb promoter
Plasmid#138274PurposeLuciferase reporter plasmid driven by TOP2A promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTOP2A promoter sequence
UseLuciferaseExpressionMammalianAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHBS1502 [IBB-GFP-mCherry3E]-[BFP-TDP43 deltaCTD]
Plasmid#133326PurposeTo test the effect of sequence on TDP43 splicing activityDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTARDBP mutant (TARDBP Human)
ExpressionMammalianMutationDeletion of CTD in full-length TDP43Available sinceNov. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1500 [IBB-GFP-mCherry3E]-[BFP-TDP43 deltaNTD]
Plasmid#133324PurposeTo test the effect of sequence on TDP43 splicing activityDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTARDBP mutant (TARDBP Human)
ExpressionMammalianMutationDeletion of NTD in full-length TDP43Available sinceNov. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
IZ401: pMVP/Lenti/Neo-DEST
Plasmid#121850PurposepMVP destination vector, empty Lentivirus vector backbone w/ Neo selection cassetteDepositorTypeEmpty backboneUseLentiviral; Pmvp gateway destination vectorAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTFAP2C-donor
Plasmid#113824PurposeCRISPR donor plasmid to tag human transcription factor TFAP2C with GFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTFAP2C homology arms flanking EGFP-IRES-Neo cassette (TFAP2C Human)
UseCRISPRMutationrs6025024, hg38:20:56638660:TTT/-Available sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-221-3p
Plasmid#103365PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-221-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-221-3p target (MIR221 Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-221-5p
Plasmid#103366PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-221-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-221-5p target (MIR221 Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only