We narrowed to 21,293 results for: T7

Showing: 9481 - 9500 of 21293 results

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pDEST14-DogCatcher

Plasmid

#171772

Purpose

Expresses DogCatcher protein in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

DogCatcher

Tags

His6 and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_EGFP_NbALFA

Plasmid

#136626

Purpose

Plasmid encoding the bacterial expression of NbALFA for ALFA-tag as an EGFP-fusion

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

EGFP-NbALFA

Tags

Hisx10 and Strep

Expression

Bacterial

Promoter

T7

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-AviTag-SnoopLigase

Plasmid

#105626

Purpose

Plasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with AviTag for biotinylation

Depositor

Mark Howarth

Article

Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

Insert

AviTag-SnoopLigase

Expression

Bacterial

Promoter

T7

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pPLV_C1

Plasmid

#186415

Purpose

Endogenous murine RNase inhibitor production for cell-free lysate

Depositor

Jerome Bonnet

Article

Voyvodic et al Sci Rep. 2022 Aug 12;12(1):13785. doi: 1

Insert

Murine RNase Inhibitor

Use

Synthetic Biology

Promoter

T7

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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mCarmine in pRSETB

Plasmid

#109484

Purpose

bacterial expression vector for mCarmine, a far red emitting fluorescent protein (excitation: 603; emission: 675)

Depositor

Oliver Griesbeck

Article

Fabritius et al Cell Chem Biol. 2018 Dec 20;25(12):1554-

Insert

mCarmine

Tags

N-terminal histidine tag in vector

Expression

Bacterial

Promoter

T7

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-dCas9-Dnmt3a-Dnmt3l-P2A-eGFP

Plasmid

#128424

Purpose

Human Codon Optimized dCas9-DNMT3A3L

Depositor

Bradley Bernstein

Article

Tarjan et al Nat Commun. 2019 Sep 18;10(1):4258. doi:

Insert

dCas9m4-sv40NLS-DNMT3A-3L-sv40NLS-P2A-eGFP

Use

CRISPR and Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

T7 Promoter

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pET26b_Nb.AT110

Plasmid

#135480

Purpose

E. coli periplasmic expression of conformational nanobody Nb.AT110 recognizing angiotensin II type 1 receptor

Depositor

Andrew Kruse

Article

Wingler et al Cell. 2019 Jan 24;176(3):479-490.e12. do

Insert

Nanobody AT110

Tags

6xHis-tag and pelB leader

Expression

Bacterial

Promoter

T7

Available Since

Oct. 8, 2020

Availability

Academic Institutions and Nonprofits only

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Ubiquitin WT

Plasmid

#12647

Depositor

Rachel Klevit

Article

Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

Insert

Ubiquitin - wild type (UBC Human)

Expression

Bacterial

Promoter

T7

Available Since

July 31, 2007

Availability

Academic Institutions and Nonprofits only

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pMBP-AsCas12a

Plasmid

#113430

Purpose

Expresses 10xHis-MBP fused to AsCas12a

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

AsCas12a

Tags

MBP

Expression

Bacterial

Promoter

T7

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pNCS BFP

Plasmid

#91757

Purpose

Bacterial expression of a fluorescent protein, Blue Fluorescent Protein (BFP)

Depositor

Erik Rodriguez, Roger Tsien

Article

Cubitt et al Trends Biochem Sci. 1995 Nov;20(11):448-

Insert

Blue Fluorescent Protein

Tags

Hexa-Histidine, Xpress epitope for detection with…

Expression

Bacterial

Mutation

GFP (Y66H)

Promoter

T7

Available Since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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pBiex-1 GFPNanobody_SNAP

Plasmid

#82711

Purpose

Expresses a GFP binding nanobody linked to a SNAP tag in bacteria and Sf9 cells.

Depositor

Sivaraj Sivaramakrishnan

Article

Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

Insert

GFP Nanobody

Tags

6xHis Purification tag, FLAG purification tag, an…

Expression

Bacterial and Insect

Promoter

T7 Promoter

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pET30b-5M SrtA with TEV site

Plasmid

#86962

Purpose

Expresses calcium-dependent Sortase A with a TEV cleavage site proximal to the C-terminal histidine tag

Depositor

Ron Bose

Article

Sarpong et al Anal Biochem. 2017 Mar 15;521:55-58. doi

Insert

S.aureus SrtA 5M

Tags

6x Histidine tag and TEV site

Expression

Bacterial

Mutation

P94R, D160N, D165A, K190E, K196T

Promoter

T7

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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pLBH531_MBP-Cas14a1 expression

Plasmid

#112500

Purpose

Expresses MBP fused to Cas14a1

Depositor

Jennifer Doudna

Article

Harrington et al Science. 2018 Oct 18. pii: science.aav42

Insert

Cas14a1

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pMBP-FnCas12a

Plasmid

#113432

Purpose

Expresses 10xHis-MBP fused to FnCas12a

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

FnCas12a

Tags

MBP

Expression

Bacterial

Promoter

T7

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_TIA1

Plasmid

#106095

Purpose

bacterial protein expression of TIA1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

TIA1 (TIA1 Human)

Tags

6xHIs-thrombin and T7

Expression

Bacterial

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pRSFDuet1_CoiA1_CoiB

Plasmid

#208761

Purpose

Precursor peptide CoiA1 and synthase CoiB, for the co-expression of CoiA1 in E.coli.

Depositor

Wilfred van der Donk

Article

Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

Inserts

CoiA1
CoiB

Tags

Hisx6 Tag and SUMO

Expression

Bacterial

Mutation

Changed Ser 20 to Asn, Changed Arg 84 to Lys, bot…

Promoter

T7

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pET-15b_T. maritima His-tRNase Z

Plasmid

#242436

Purpose

Expresses T. maritima tRNase Z in E. coli.

Depositor

Sebastian Maerkl

Article

Li et al Nat Commun. 2025 Jul 5;16(1):6212. doi:

Insert

tRNase Z

Tags

6x His

Expression

Bacterial

Promoter

T7

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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CLIPf-KIF5B-SNAPf-HisTag-FLAG

Plasmid

#200799

Purpose

Mammalian expression of CLIPf-KIF5B-SNAPf-HisTag-FLAG

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2024.12.20.629623

Insert

KIF5B (KIF5B Human)

Tags

CLIPf and SNAPf, HisTag, FLAG

Expression

Mammalian

Promoter

CMV & T7

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD-BirA-eCPX-AP

Plasmid

#121907

Purpose

Expresses BirA and eCPX-AP

Depositor

Per Svenningsen

Article

Granhoj et al Sci Rep. 2019 Mar 11;9(1):4118. doi: 10.

Inserts

BirA (birA Synthetic)
Enhanced Circularly Permuted Outer Membrane Protein X

Tags

6xHis

Expression

Bacterial

Promoter

T7 and pBAD promoter

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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SpyCatcher002-oDi

Plasmid

#124661

Purpose

Expresses SpyCatcher002-oDi for dimerization of SpyTag-proteins via dimeric coiled-coil

Depositor

Mark Howarth

Article

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyCatcher002-oDi

Tags

C-tag, His6, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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