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PADH1-TIR1-9myc-URA3

Plasmid

#207016

Purpose

Expresses TIR1-9Myc. TIR1 mediates auxin-induced ubiquitination of auxin-inducible degron (AID).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

TIR1

Tags

9xMyc

Expression

Yeast

Promoter

pADH1

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR-ARF19

Plasmid

#83846

Purpose

Gateway donor vector for ARF19

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

ARF19 (ARF19 Mustard Weed)

Use

Gateway donor vector

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pEM112B

Plasmid

#210602

Purpose

Reporter gene expression in B. dendrobatidis

Depositor

Lillian Fritz-Laylin

Article

Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Insert

SpH2Ater-mClover3-SpH2Apro | SpH2Bpro-GP2A-GT2A-Hshph-Synter8

Tags

mClover3

Expression

Bacterial and Yeast

Promoter

SpH2A/Bpro (divergent)

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PM-CaNAR2

Plasmid

#64730

Purpose

Plasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

Lyn-CaNAR2

Tags

Cerulean3, N-terminal targeting sequence from Lyn…

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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3410 pBM272 Bcl-XL

Plasmid

#8772

Depositor

Stanley Korsmeyer

Article

Gross et al Mol Cell Biol 2000 May;20(9):3125-36.

Insert

Bcl-XL (Bcl2l1 Mouse)

Expression

Yeast

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
Human NCKX3-FlagTag (pcDNA3.1-)

Plasmid

#75206

Purpose

Mammalian expression of SLC24A3

Depositor

Jonathan Lytton

Article

Kraev et al J Biol Chem. 2001 Jun 22;276(25):23161-7

Insert

SLC24A3 (SLC24A3 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pEK36

Plasmid

#210598

Purpose

Reporter gene expression in B. dendrobatidis

Depositor

Lillian Fritz-Laylin

Article

Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Insert

SpH2Bpro-Hshph-G4S-HsmClover3-ScADH1ter

Tags

mClover3

Expression

Bacterial and Yeast

Promoter

SpH2Bpro

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pET16b-MYPT1(1-314)

Plasmid

#224893

Purpose

Bacterial expression of MYPT1(1-314)

Depositor

Mathieu Bollen

Article

Claes et al Cell Chem Biol. 2023 Dec 21;30(12):1666-

Insert

myosin phosphatase targeting subunit 1 (PPP1R12A )

Expression

Bacterial

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pCFJ1410 - [1-2] ENTR - Fluor - mCherry(PATCs, NLS(2), no_atg, no_stop)

Plasmid

#159859

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - mCherry(PATCs, NLS(2), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pMRE154

Plasmid

#118504

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mOrange2

Expression

Bacterial

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-3xGFP11-mem-3xControlgRNA

Plasmid

#224583

Purpose

Ubiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.

Depositor

Erica Hutchins

Article

Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

Insert

3xGFP11

Use

CRISPR

Tags

Membrane Localization Signal

Mutation

Codon optimized

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P3nmt1

Plasmid

#39280

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Mutation

-1163 to +6 of nmt1 locus

Available Since

Aug. 14, 2012

Availability

Academic Institutions and Nonprofits only

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myc-hAtg13

Plasmid

#31965

Depositor

Do-Hyung Kim

Article

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Insert

Atg13 (ATG13 Human)

Tags

myc

Expression

Mammalian

Promoter

CMV, SP6

Available Since

Sept. 1, 2011

Availability

Academic Institutions and Nonprofits only
pCMV E2F1 E132

Plasmid

#24224

Purpose

Mammalian expression of human E2F1 (DNA binding mutant) with HA tag

Depositor

Kristian Helin

Article

Helin et al Genes Dev. 1993 Oct . 7(10):1850-61.

Insert

E2F1 E132 (E2F1 Human)

Expression

Mammalian

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pLEX_970_puro_DEST_V5_SKIL

Plasmid

#45289

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

SNON (SKIL Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-Mito-CaNAR2

Plasmid

#64731

Purpose

Mitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of mitochondrial outer membrane.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CaNAR2

Tags

Cerulean3, N-terminal 30 amino acids from DAKAP1,…

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pRS315e_pGal-dCas9-PmCDA1

Plasmid

#79615

Purpose

Expresses dCas9-PmCDA1 in yeast cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Tags

PmCDA1

Expression

Yeast

Mutation

D10A and H840A for nuclease deficient Cas9

Promoter

pGal1

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-ChRger2-TS-EYFP

Plasmid

#127238

Purpose

High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.

Depositor

Viviana Gradinaru

Article

Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

ChRger2-TS-EYFP

Use

AAV

Tags

EYFP and SpyTag

Expression

Mammalian

Promoter

CamKIIa

Available Since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMRE161

Plasmid

#118509

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mTurquoise2

Expression

Bacterial

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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BS-TRP1-TetOff(7 repeats)-mRuby3-Pho8

Plasmid

#207031

Purpose

Tet-Off controlled mRuby3-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Pho8

Tags

mRuby3

Expression

Yeast

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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