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pLeo-A-a2-GEMS(JAK-STAT)

Plasmid

#209110

Purpose

Transient mammalian expression of the CC functionalized GEMS receptor A-a2-GEMS (JAK-STAT)

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

A-a2-GEMS(JAK-STAT)

Expression

Mammalian

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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NF-155 pcDNA3

Plasmid

#197290

Purpose

Plasmid expressing an antigen targeting the Plasmid expressing an antigen targeting Neurofascin-155

Depositor

Peter Brophy

Article

Collinson et al Glia. 1998 May;23(1):11-23

Insert

Pan-Neurofascin (extracellular) (Nfasc Rat)

Expression

Mammalian

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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pTS94 pHAGE2 CMVtetO2 GFP-22xGS-3C-MCS

Plasmid

#199347

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-3C-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP-3C

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pRC3EGFP

Plasmid

#193790

Purpose

Expresses EGFP under the control of PR promoter, p15A origin of replication, Chloramphenicol selection

Depositor

Sangram Bagh

Article

Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

Insert

EGFP

Expression

Bacterial

Promoter

PR promoter

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pENeGFP PCNAL2 pc653

Plasmid

#167564

Purpose

The GFP-PCNAL2 fusion protein contains a SV40 nuclear localization signal at the NH2 terminus followed by an enhanced mutant GFP gene that is fused to the human PCNA by a linker.

Depositor

Cristina Cardoso

Article

Leonhardt et al J Cell Biol. 2000 Apr 17;149(2):271-80.

Insert

Proliferating Cell Nuclear Antigen (PCNA Human)

Tags

GFP

Expression

Mammalian

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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gRNAy

Plasmid

#168294

Purpose

Expresses gRNA targeting the yellow gene in Drosophila

Depositor

Omar Akbari

Article

Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

Insert

U6.3-gRNA[y]-scaffold

Expression

Insect

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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p15a_Kan_MLS_Rosa26_Short_CRISPRDel_CAG_FNeoStop_WPRE_bgh_pA

Plasmid

#97010

Purpose

Rosa26 targeting vector for FLP-responsive cassette

Depositor

Russell Ray

Article

Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

Type

Empty backbone

Use

CRISPR and Cre/Lox

Expression

Mammalian

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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p15a_Kan_MLS_Rosa26_Short_CRISPRDel_FNeoStop_PStop_WPRE_bgh_pA

Plasmid

#97012

Purpose

Rosa26 targeting vector for intersectional Cre and FLP responsive cassette

Depositor

Russell Ray

Article

Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

Type

Empty backbone

Use

CRISPR and Cre/Lox

Expression

Mammalian

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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JEH127

Plasmid

#179299

Purpose

Expresses dCas9 fused to VPR

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

Insert

dCas9-VPR

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive Cas9)

Promoter

CAG

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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SNX12-PX (1-162)

Plasmid

#119093

Purpose

Bacterial expression of human phox homology (PX) domain, SNX12-PX (1-162)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX12-PX (1-162) (SNX12 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pJH2124

Plasmid

#178791

Purpose

Prgef-1 EGL-3 unc-54 3' UTR C.elegans pan-neural expression of EGL-3

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

EGL-3 (egl-3 Nematode)

Expression

Worm

Promoter

Prgef-1

Available Since

Jan. 5, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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CTAG_mScarlet-I

Plasmid

#136118

Purpose

FP for C-term fusion with CDS12

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG-mScarletI(noATG)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pME-nCas9n-P2A-TagBFP2-pA

Plasmid

#176026

Purpose

Multisite gateway vector for expression of nCas9n with blue reporter. Parton lab clone ENT

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

nCas9n-P2A-TagBFP2

Use

Gateway Cloning

Available Since

Oct. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pN1-NLS-QUE37C

Plasmid

#129323

Purpose

ATP measurement, nucleus

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN-37C

Expression

Mammalian

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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Integrin-αv Ectodomain M400C

Plasmid

#159642

Purpose

Contains integrin alpha v residues 1–960 with the M400C mutation

Depositor

Timothy Springer

Article

Wang et al Proc Natl Acad Sci U S A. 2017 May 23;11

Insert

Integrin alpha v ectodomain

Expression

Mammalian

Mutation

M400C

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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SNX5-PX (26-170)

Plasmid

#119086

Purpose

Bacterial expression of human phox homology (PX) domain, SNX5-PX (26-170)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX5-PX (26-170) (SNX5 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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VAMP8-SuperNova

Plasmid

#131855

Purpose

Expresses SuperNova fused to endosomal SNARE VAMP8

Depositor

Geert van den Bogaart

Article

Paardekooper et al Int J Mol Sci. 2019 Aug 25;20(17). pii:

Insert

SuperNova

Tags

Myc-tag and VAMP8 (mouse sequence)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b- mKate2-KDEL-pA

Plasmid

#109494

Purpose

Muscle specific zebrafish expression of mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actc1b- mKate2-KDEL-pA

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pOGG043

Plasmid

#133123

Purpose

Level 0 PU module synthesised as a fragment (pL0M-PU-PsNifH). Synthetic consensus promoter to drive nodule-specific expression in biovars and strains of R. leguminosarum.

Depositor

Philip Poole

Article

Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

Insert

PsNifH Synthetic consensus nifH promoter

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pOGG008

Plasmid

#113982

Purpose

Vector construction module. Kanamycin/neomycin resistance module for construction of Golden Gate cloning vectors

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Kanamycin/neomycin

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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