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p2CT-His-MBP-Hhe_Cas13a_WT

Plasmid

#91871

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Publication

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

HheCas13a (HheC2c2) WT

Tags

His6-MBP-TEV site

Expression

Bacterial

Available since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA-Flag-RCC1-polyA

Plasmid

#59762

Purpose

Express Flag-tagged RCC1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Publication

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

RCC1

Expression

Mammalian

Promoter

CMV and T7

Available since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pUC57-Rluc-A114-N40

Plasmid

#50561

Purpose

Template DNA for in vitro transcription using T7 polymerase

Depositor

Yukihide Tomari

Publication

Fukaya et al EMBO J. 2011 Nov 25;30(24):4998-5009. do

Insert

Luciferase-A114-N40

Use

Cloning vector

Promoter

lac

Available since

Feb. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pBabe_puro_DEST_Flag_PHC3

Plasmid

#45262

Depositor

William Hahn

Publication

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

PHC3 (PHC3 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available since

May 23, 2013

Availability

Academic Institutions and Nonprofits only
TFORF3386

Plasmid

#144862

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Publication

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

IRF4 (IRF4 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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p3E ubb polyA

Plasmid

#195977

Purpose

Gateway p3E vector

Depositor

Alexa Burger, Christian Mosimann

Publication

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

ubb:polyA (ubb Zebrafish)

Use

Other

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_LAMP1_HaloTag9

Plasmid

#175541

Purpose

Lyzosomal expression of HaloTag9 in mammalian cells

Depositor

Kai Johnsson

Publication

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

LAMP1-HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pM1s3AsR_TS-C7

Plasmid

#137945

Purpose

E. coli Nissle pMUT1-derived plasmid with ampicillin resistance and constitutive RFP expression and strong temperature sensitive GFP expression

Depositor

Neel Joshi

Publication

Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

Insert

mRFP1

Use

Synthetic Biology; For use with e. coli nissle in…

Expression

Bacterial

Promoter

J23101

Available since

April 27, 2021

Availability

Academic Institutions and Nonprofits only

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TGT-His

Plasmid

#138201

Purpose

Expresses E. Coli TGT with a C-terminal His Tag

Depositor

Neal Devaraj

Publication

Busby et al Methods Enzymol. 2020;641:373-399. doi:

Insert

E Coli tRNA Guanine Transglycosylase-His

Tags

His

Expression

Bacterial

Promoter

T7 promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC33A1

Plasmid

#132264

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC33A1 (SLC33A1 Human)

Expression

Mammalian

Available since

Nov. 19, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pDONR221_SLC9A1

Plasmid

#132210

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC9A1 (SLC9A1 Human)

Expression

Mammalian

Available since

Oct. 28, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pMB22

Plasmid

#122244

Purpose

Lentivirus delivery for stable expression of AsCas12a-VPR

Depositor

Darjus Tschaharganeh

Publication

Breinig et al Nat Methods. 2019 Jan;16(1):51-54. doi:

Insert

mammalian codon-optimized Acidaminococcus sp. Cas12a fused to VPR activator

Use

Lentiviral

Tags

NLS and VPR activator

Promoter

EFs

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pEB1-mGFPmut3

Plasmid

#103981

Purpose

Plasmid encoding mGFPmut3

Depositor

Philippe Cluzel

Publication

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mGFPmut3

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S65G, S72A, …

Promoter

proC

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUD628

Plasmid

#103018

Purpose

expression of a Cpf1 programming crRNA targetting ADE2 (crADE2-3.S)

Depositor

Jean-Marc Daran

Publication

Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

Insert

crADE2-3.S (ADE2 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pEX-A-U6-TelgRNA_PuroR

Plasmid

#84782

Purpose

Expresses telomere-specific sgRNA.

Depositor

Heinrich Leonhardt

Publication

Schmidtmann et al Nucleus. 2016 Sep 27:0.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

telomere-specific sgRNA

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

U6

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJPC12-ΔM13-mCherry-PR/PRM-GFP

Plasmid

#80859

Purpose

Reporter plasmid (mCherry and GFP)

Depositor

Mark Isalan

Publication

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

mCherry
GFP

Expression

Bacterial

Promoter

Lambda Pr and Lambda Prm

Available since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMpGWB403

Plasmid

#68668

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Publication

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Expression

Plant

Promoter

MpEF1alpha promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAR-9.47_VP2_AS

Plasmid

#74241

Purpose

Original plasmid described in Choudhury et al, 2015 Mol. Ther. for production of AAV-AS vectors. Encodes VP2_AS chimeric protein with 19 alanines fused to the N-terminus of AAV9.47 VP2.

Depositor

Miguel Sena-Esteves

Publication

Choudhury et al Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2

Inserts

AAV2 Rep
AAV9.47-VP2_AS

Use

AAV

Tags

19 alanines fused to VP2 of AAV9.47

Available since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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Wnt3A STOP

Plasmid

#35871

Depositor

Marian Waterman

Publication

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt3A (WNT3A Human)

Use

Gateway Cloning

Mutation

None

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pLVNP3.0-PEmax

Plasmid

#206883

Purpose

Expresses FLAG-tagged PEmax fused to Gag through a linker sequence

Depositor

Jacob Giehm Mikkelsen

Publication

Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

Insert

PEmax

Use

CRISPR and Lentiviral

Tags

FLAG

Promoter

CMV

Available since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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