We narrowed to 12,981 results for: NUC
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pLVX-CMV-Flag-PolB(K206A/K244A/T304I)-Puro
Plasmid#177145PurposeLentiviral vector expressing Flag-PolB(K206A/K244A/T304I) and a puromycin resistance cassetteDepositorAvailable SinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-GST-PolB(L301R/V303R/V306R)
Plasmid#177137PurposeBacterial vector for expression of an N-terminal GST fusion of PolB(L301R/V303R/V306R) with a TEV protease site located between the GST tag and PolB(L301R/V303R/V306R)DepositorAvailable SinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-GST-PolB(K206A/K244A/T304I)
Plasmid#177134PurposeBacterial vector for expression of an N-terminal GST fusion of PolB(K206A/K244A/T304I) with a TEV protease site located between the GST tag and PolB(K206A/K244A/T304I)DepositorAvailable SinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delZn3-L713F
Plasmid#173934PurposeBacterial expression of PARP1 lacking Zn3 domain but containing L713F gain-of-function mutationDepositorInsertPARP1delZn3-L713F (PARP1 Human)
Tags6xHisExpressionBacterialMutationDeletion of residues 214-373; L713F, V762AAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-Cn-VA-NHEJ1-S262E/S263E
Plasmid#141342PurposeLentiviral vector for expression of human NHEJ1-S262E/S263E in mammalian cellsDepositorInsertNHEJ1-S262E/S263E (NHEJ1 Human)
UseLentiviralTagsVA tag (3XFLAG-2XTEV-6XHis-2XStrep-Beacon)ExpressionMammalianMutationS262E/S263EPromoterCMVAvailable SinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-Cn-VA-NHEJ1-S262A/S263A
Plasmid#141343PurposeLentiviral vector for expression of human NHEJ1-S262A/S263A in mammalian cellsDepositorInsertNHEJ1-S262A/S263A (NHEJ1 Human)
UseLentiviralTagsVA tag (3XFLAG-2XTEV-6XHis-2XStrep-Beacon)ExpressionMammalianMutationS262A/S263APromoterCMVAvailable SinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH_CMV_HMGB_ctail_mKate2_CLEAVAGE
Plasmid#167338PurposeExpresses mKate2 fused to TFAM HMGB+C-tail mutant in mammalian cellsDepositorInsertTFAM (TFAM Human)
UseLentiviralTagsmKate2ExpressionMammalianMutationTruncation mutant containing MTS (1-42) as well a…PromoterCMVAvailable SinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSCV-HA-Nr4a1-Zf2
Plasmid#160944PurposeGeneration of retrovirus for the overexpression of Nr4a1 mutant (zinc finger 2 deletion)DepositorInsertNr4a1 (Nr4a1 Mouse)
UseRetroviralTagsHA tagExpressionMammalianMutationDeletion of Zinc finger 2 (C306 to C330)PromoterMSCV-LTRAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV-HA-Nr4a1-Zf1
Plasmid#160943PurposeGeneration of retrovirus for the overexpression of Nr4a1 mutant (zinc finger 1 deletion)DepositorInsertNr4a1 (Nr4a1 Mouse)
UseRetroviralTagsHA tagExpressionMammalianMutationDeletion of Zinc finger 1 (C270 to C290)PromoterMSCV-LTRAvailable SinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DDX43-NT-G46D
Plasmid#134588PurposeExpress human DDX43 N-terminal region (1-253 aa, with G46D mutation) in E. coliDepositorInsertDDX43-N-terminal (1-253 aa) with G46D mutation (DDX43 Human)
TagsHisExpressionBacterialMutationfull-length (G154D)PromoterT7Available SinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DDX43-FL-G84D
Plasmid#134577PurposeExpress human DDX43 mutant (full-length with G84D mutation) in E. coliDepositorInsertDDX43-G84D (DDX43 Human)
TagsHisExpressionBacterialMutationfull-length (G84D)PromoterT7Available SinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
gRNA-NONO-1
Plasmid#127654PurposeKnock-out of human NONO (guide only)DepositorInsertNONO sgRNA (NONO Human)
ExpressionMammalianAvailable SinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28B-PU.1-ETS-Wing
Plasmid#124552PurposeChimeric ETS domain: Wing of Ets-1 in PU.1 scaffoldDepositorTags6xHis tagExpressionBacterialMutationThe 5 residues making up the "wing" in …Available SinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMCh-1540
Plasmid#82433PurposePlasmid for expression of GST-tagged human-Nematostella GW182 11W11A chimera in mammalian cellsDepositorInserths-nvGW182 11W11A chimera
TagsGSTExpressionMammalianMutationaa 1-1169 of siRNA resistant hsTNRC6A (Q8NDV7-2) …PromoterEF1AAvailable SinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMSCV40-EF1a-hBCL10(1-114)-SO
Plasmid#75257Purposeretroviral expression vector for expression of StrepOne-Tagged human BCL10 (AS 1-116)DepositorInsertBcl10 (BCL10 Human)
UseRetroviralTagsStrepOne TagExpressionMammalianMutationAS 1-116 (CARD-domain)PromoterpMSCV-LTRs, EF1aAvailable SinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
Helios-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)
Plasmid#75246PurposeCRISPR/Cas9 NICKASE plasmid against human Helios (1/2)DepositorInsertsgRNA against human Helios (IKZF2 Human)
UseCRISPRAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
Helios-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)
Plasmid#75247PurposeCRISPR/Cas9 NICKASE plasmid against human Helios (2/2)DepositorInsertsgRNA against human Helios (IKZF2 Human)
UseCRISPRAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-M-4F2
Plasmid#73431PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 4F2.DepositorInsertRepressor 4F2 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterConstitutive wild-type S. pyogenes promoterAvailable SinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-M-1B6
Plasmid#73430PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1B6.DepositorInsertRepressor 1B6 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterConstitutive wild-type S. pyogenes promoterAvailable SinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only
