We narrowed to 8,402 results for: 221
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Plasmid#208826PurposeThe SSA-based surrogate reporter for validating the activity of gene editing tools as well as for helping to screen gene-editing positive cells. Key Construct: CAG-PuroR-CMV-eGFP*(SSA).DepositorTypeEmpty backboneExpressionMammalianPromoterCMVAvailable SinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only
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pCDNA4/TO-GFP-SPOP (R221C)
Plasmid#197009PurposeMammalian inducible expression vector for GFP-tagged SPOPDepositorAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4/TO-SPOP-myc (R221C)
Plasmid#197005PurposeMammalian inducible expression vector for myc-tagged SPOPDepositorAvailable SinceApril 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpH-HsNot10_221-404_Q
Plasmid#147303PurposeBacterial Expression of HsNot10_221-404DepositorInsertHsNot10_221-404 (CNOT10 Human)
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-WT-short-UBASH3A-Ctag
Plasmid#192099PurposeHuman wild-type short-form UBASH3A in Gateway pDONR221 (for C-terminal tag)DepositorInsertUBASH3A (UBASH3A Human)
ExpressionMammalianAvailable SinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-WT-short-UBASH3A-Ntag
Plasmid#192100PurposeHuman wild-type short-form UBASH3A in Gateway pDONR221 (for N-terminal tag)DepositorInsertUBASH3A (UBASH3A Human)
ExpressionMammalianAvailable SinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HUWE1_UBA/UIM-mut
Plasmid#187151PurposeEntry cloneDepositorAvailable SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HUWE1_YH/GG_delta3291-3298
Plasmid#187126PurposeEntry cloneDepositorAvailable SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJEP221-AAV-RSV-GFP-pA
Plasmid#62919PurposeAAV vector backbone designed to express GFP from a RSV promoter. It also contains a poly-Adenylation Signal (pA)DepositorInsertGreen Fluorescent protein (GFP)
UseAAVPromoterRous Sarcoma Virus(RSV)Available SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 FIIND L370G
Plasmid#169970PurposepDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F370G)DepositorAvailable SinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 FIIND L368G
Plasmid#169969PurposepDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (L368G)DepositorAvailable SinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 PAM L368G
Plasmid#169978PurposepDONR221 for Gateway shuttling. Contains CARD8 with a site III mutation (L368G) with sgRNA binding site mutationsDepositorAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 PAM R394E
Plasmid#169980PurposepDONR221 for Gateway shuttling. Contains CARD8 with a site III mutation (R394E) with sgRNA binding site mutationsDepositorAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 PAM S297A
Plasmid#169976PurposepDONR221 for Gateway shuttling. Contains CARD8 that is autoprocessing-deficient (S297A) with sgRNA binding site mutationsDepositorAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 FIIND E274R
Plasmid#169967PurposepDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) that cannot bind DPP9 at site I (E274R)DepositorAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 FIIND R394E
Plasmid#169971PurposepDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (R394E)DepositorAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 FIIND F405G
Plasmid#169973PurposepDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F405G)DepositorAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 PAM F370G
Plasmid#169979PurposepDONR221 for Gateway shuttling. Contains CARD8 with a site III mutation (F370G) with sgRNA binding site mutationsDepositorAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 FIIND S297A
Plasmid#169966PurposepDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon)DepositorAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 PAM F405G
Plasmid#169981PurposepDONR221 for Gateway shuttling. Contains CARD8 with a site III mutation (F405G) with sgRNA binding site mutationsDepositorAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 PAM E274R
Plasmid#169977PurposepDONR221 for Gateway shuttling. Contains CARD8 that cannot bind DPP9 due to a mutation at site I (E274R) with sgRNA binding site mutationsDepositorAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR 221-PHL2 (STOP)
Plasmid#118683PurposeGateway entry vectorDepositorInsertPHL2 (AT3G24120 Mustard Weed)
UseOtherAvailable SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR 221-XERICO (STOP)
Plasmid#118685PurposeGateway entry vectorDepositorInsertXERICO (XERICO Mustard Weed)
UseOtherAvailable SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR 221-BPC4 (STOP)
Plasmid#118680PurposeGateway entry vectorDepositorInsertBPC4 (BPC4 Mustard Weed)
UseOtherAvailable SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 NLRP1 delta787-843
Plasmid#166821PurposeGateway pDONR vector encoding NLRP1 with the patient mutation _787-843 (Zhong et al., 2016) (no stop codon)DepositorAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 Ub-NLRP1 S1213 P1214R
Plasmid#166830PurposepDONR221 encoding ubiquitin-tagged NLRP1-CT to generate the native S1213 N-terminus, with the P1214R patient mutation (Grandemange et al., 2017) (no stop)DepositorAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-TAF15 g1172a No Stop
Plasmid#84898PurposeDONOR vector for Gateway cloning of TAF15 g1172a No StopDepositorAvailable SinceApril 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-EWSR1 g1750a No Stop
Plasmid#84894PurposeDONOR vector for Gateway cloning of EWSR1 g1750a No StopDepositorAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-EWSR1 g1750a Stop
Plasmid#84893PurposeDONOR vector for Gateway cloning of EWSR1 g1750a StopDepositorAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-EWSR1 c1655t Stop
Plasmid#84891PurposeDONOR vector for Gateway cloning of EWSR1 c1655t StopDepositorAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-hSOX10-G1238A-C1240T
Plasmid#24739DepositorInsert(sex determining region Y)-box 10 (SOX10 Human)
UseGateway donor vectorMutationG replaced with A at position 1238 and C replaced…Available SinceMay 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-Lifeact- mScarlet-HA (JDW 1247)
Plasmid#224538PurposeA Gateway compatible middle entry clone containing Lifeact-mScarlet-I (far red f-actin reporter)DepositorInsertLifeact-mScarlet-HA
UseGateway cloningAvailable SinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7NT*-Bri2 113-231 R221E
Plasmid#138134PurposeExpresseds human Bri2 BRICHOS R221E mutant in E. coliDepositorInserthuman Bri2 BRICHOS R221E (ITM2B Human)
TagsNT* tag derived from spider silk proteinsExpressionBacterialPromoterT7Available SinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ108 - pENTR221 - [cbr-unc-119(+)]
Plasmid#200367Purposecbr-unc-119 rescue gene for use as co-injection marker.DepositorInsert[cbr-unc-119(+)]
ExpressionWormAvailable SinceJune 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-H1-sgGFP1-U6-sgGFP2-7SK-sgGFP3
Plasmid#87906PurposesgRNA targeting GFPDepositorInsertsgGFP1, sgGFP2, sgGFP3
ExpressionMammalianPromoterH1, U6, 7SKAvailable SinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
JDW 743 (pDONR221-EYFP-hKRAS4B-WT)
Plasmid#156402PurposeGateway middle entry clone encoding EYFP-fused to human KRAS4B, includes a stop codonDepositorAvailable SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-H1-sgHTT1-U6-sgGFP2-7SK-sgCas9
Plasmid#87917PurposeGateway cloningDepositorInsertsgHTT1, sgGFP2, shCas9
ExpressionMammalianPromoterH1, U6, 7SKAvailable SinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
221-RPB1-AID-eGFP-2A-blasticidin
Plasmid#195107PurposeTargeting vector to introduce AID-eGFP tags in C-terminal of the human RPB1 gene. Blasticidin selection (2A-fusion). Auxin-inducible degron system.DepositorInsertAID-eGFP
ExpressionMammalianAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
VV221: sdChR(C138S)-TS-eGFP-ER in fck
Plasmid#58513PurposeExpresses sdChR with the C138S (step function opsin) mutation fused to eGFP under the a-CamKII promoterDepositorInsertsdChR(C138S)-TS-eGFP-ER
UseLentiviralTagseGFPExpressionMammalianMutationchanged Cysteine 138 to SerinePromotera-CamKIIAvailable SinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
JDW 822 (pDONR221-V5-mScarlet-I-KRAS4A-WT)
Plasmid#156405PurposeGateway middle entry clone encoding V5-tagged mScarlet-I-fused to human KRAS4A, includes a stop codonDepositorAvailable SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-rap1-K462-463-651R
Plasmid#84564PurposeThis plasmid can be used as a donor for subcloning into Gateway destination vectors.DepositorAvailable SinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-rap1-K43-117-118R
Plasmid#84565PurposeThis plasmid can be used as a donor for subcloning into Gateway destination vectors.DepositorAvailable SinceOct. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
SOX D221A/E244A/K246A pACEmam1
Plasmid#192050PurposeExpresses a 3X catalytic mutant of SOX (ORF37)DepositorInsertSOX (3X catalytic mutant)
UseUnspecifiedMutation3X catalytic mutant D221A/E244A/K246APromoterCMVAvailable SinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACE SOX (ORF37) D221N E244Q
Plasmid#192053PurposeExpresses a 2X catalytic mutant of (ORF37) D221N E244QDepositorInsertSOX (D221N E244Q)
UseUnspecifiedMutation2X catalytic mutant D221N E244QPromoterCMVAvailable SinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3E-WPRE-bGH-pA (JDW 1221)
Plasmid#224527PurposeA Gateway compatible 3' entry clone containing a WPRE upstream of Bovine growth hormone polyA to stabilize mRNDepositorInsertWPRE-bGH
UseGateway cloningAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 msNLRP3(ΔPYD, I125M-END)
Plasmid#218641PurposeGateway shuttling vector for cloning N- and C-terminal NLRP3(ΔPYD, I125M-END) tags (contains no stop codon)DepositorAvailable SinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-K40R-short-UBASH3A-Ntag
Plasmid#195401PurposeHuman K40R short-form UBASH3A in Gateway pDONR221 (for N-terminal tag)DepositorAvailable SinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-K40R-short-UBASH3A-Ctag
Plasmid#195400PurposeHuman K40R short-form UBASH3A in Gateway pDONR221 (for C-terminal tag)DepositorAvailable SinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-deltaUBA-short-UBASH3A-Ctag
Plasmid#195397PurposeHuman short-form UBASH3A lacking the UBA domain in Gateway pDONR221 (for C-terminal tag)DepositorInsertUBASH3A (UBASH3A Human)
UseGateway donor vectorMutationUBASH3A mutant lacking the UBA domainAvailable SinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-W279A-short-UBASH3A-Ctag
Plasmid#195402PurposeHuman W279A short-form UBASH3A in Gateway pDONR221 (for C-terminal tag)DepositorAvailable SinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only