We narrowed to 1,274 results for: AP2

1. pRetroX-TRE3G-IRSp53 S269D

   Plasmid

   #221319

   Purpose

   Dox-inducible expression of IRSp53 mutant in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

   Insert

   IRSp53 (Baiap2 Mouse)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mutation

   S269D

   Promoter

   TRE3G

   Available since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRetroX-TRE3G-IRSp53

   Plasmid

   #221317

   Purpose

   Dox-inducible expression of IRSp53 in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

   Insert

   IRSp53 (Baiap2 Mouse)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-FKBP-IRSp53(pLxIS)

   Plasmid

   #221336

   Purpose

   Overexpression of IRSp53 pLxIS domain in bacteria

   Depositor

   Jonathan Kagan

   Article

   Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

   Insert

   IRSp53 pLxIS domain (Baiap2 Mouse)
   

   Use

   Tags

   6xHis-4xFKBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. VIPR2-DuET

   Plasmid

   #213387

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   VIPR2 (VIPR2 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. TFORF2281

   Plasmid

   #142929

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   MYBBP1A (MYBBP1A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. TFORF2282

   Plasmid

   #142400

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   MYBBP1A (MYBBP1A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF0374

   Plasmid

   #141595

   Purpose

   Lentiviral vector for overexpressing the NFYA transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NFYA (NFYA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. p8322 pDONR ATG-YAP1 S94A-Stop

   Plasmid

   #184526

   Purpose

   Subcloning YAP1 S94A

   Depositor

   Elizabeth White

   Article

   Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

   Insert

   YAP1 S94A (YAP1 Human)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   S94A

   Promoter

   Available since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. p8321 pDONR ATG-YAP1 S127A-Stop

   Plasmid

   #184525

   Purpose

   Subcloning YAP1 S127A

   Depositor

   Elizabeth White

   Article

   Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

   Insert

   YAP1 S127A (YAP1 Human)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   S127A

   Promoter

   Available since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHH0103_GRB2_SH3

    Plasmid

    #110015

    Purpose

    Protein expression and purification of GRB2_SH3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    GRB2_SH3 (GRB2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. GRB2-SHN

    Plasmid

    #139625

    Purpose

    StrepIII-HA-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

    Insert

    GRB2 (GRB2 Human)
    

    Use

    Tags

    StrepIII-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFL His-I3

    Plasmid

    #113513

    Purpose

    Expression of His-I3 in insect cells (Baculovirus system)

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    I3 (PPP1R11 Human)
    

    Use

    Tags

    6*His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEN_TTmiRc2 3XFLAG-MKK4-EE-NLS

    Plasmid

    #87776

    Purpose

    Used as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    MKK4 (MAP2K4 Human)
    

    Use

    Tags

    3xFLAG and NLS

    Expression

    Mutation

    Promoter

    Available since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-Flag-YAP-H122Y

    Plasmid

    #33085

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

    Insert

    YAP (YAP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    H122Y

    Promoter

    CMV

    Available since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pLX307 P2A GFP YAP1

    Plasmid

    #193675

    Purpose

    Constitutive lentiviral expression of YAP1

    Depositor

    William Hahn

    Article

    Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. TetON-shYAP

    Plasmid

    #115667

    Purpose

    Inducible YAP knockdown

    Depositor

    Joan Massague

    Article

    Er et al Nat Cell Biol. 2018 Aug;20(8):966-978. d

    Insert

    YAP shRNA (YAP1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR221-YAP1 CA

    Plasmid

    #79502

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human YAP1 S127A mutant CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Multisite gateway entry clone

    Tags

    3XFLAG

    Expression

    Mutation

    S127A

    Promoter

    no Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMXs2-MEK1

    Plasmid

    #86141

    Purpose

    Retroviral vector expressing MEK1-IRES-Bsd-p2A-RFP

    Depositor

    David Sabatini

    Article

    Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

    Insert

    MEK1 (MAP2K1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. FLAG-Mkk7-JNK2 fusion-pcw107

    Plasmid

    #64618

    Purpose

    Mkk7-JNK2 fusion - barcoded activating construct of JNK pathway

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    Map2k7 linker MAPK9
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    mouse-human fusion

    Promoter

    PGK

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pLVX-TetOne-Puro-p21

    Plasmid

    #171122

    Purpose

    Doxycycline inducible expression of p21

    Depositor

    Jason Sheltzer

    Article

    Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

    Insert

    p21 (CDKN1A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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