We narrowed to 2,122 results for: D10

1. Lenti-EF1alpha-dCas9-VP64-MS2-VP64-ZsG

   Plasmid

   #192665

   Purpose

   3rd generation lenti vector encoding dCas9-VP64 with P2A MS2-VP64 and T2A ZsGreen1

   Depositor

   Jeffrey Miner

   Insert

   dCas9-VP64, MS2-VP64, ZsGreen1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9

   Promoter

   EF1a

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Bsrs103-RpaR-LasI

   Plasmid

   #85160

   Purpose

   Constitutively expresses RpaR and inducibly expresses LasI

   Depositor

   Jeff Hasty

   Article

   Scott et al ACS Synth Biol. 2016 Sep 16;5(9):969-77.

   Insert

   RpaR, LasI
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pScarlet-Kif5a

   Plasmid

   #118744

   Purpose

   Expresses scarlet tagged Kif5a

   Depositor

   Beverly Koller

   Insert

   Kif5a cDNA (Kif5a Mouse)
   

   Use

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-EF1alpha-dCas9-VP64-MS2-VP64-Blast

   Plasmid

   #192666

   Purpose

   3rd generation lenti vector encoding dCas9-VP64 with P2A MS2-VP64 and T2A Blast resistance marker

   Depositor

   Jeffrey Miner

   Insert

   dCas9-VP64, MS2-VP64
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9

   Promoter

   EF1a

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRDA_867

   Plasmid

   #231109

   Purpose

   A>G base editor

   Depositor

   John Doench, David Root

   Insert

   ABE8e-nCas9-SpG-HA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   D10A

   Promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRDB_270

   Plasmid

   #231111

   Purpose

   C>T base editor

   Depositor

   John Doench, David Root

   Insert

   TadA-CDd-nCas9-SpG-UGI-HA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   D10A

   Promoter

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-BE4max-P2A-EGFP (pRZ33)

   Plasmid

   #140083

   Purpose

   CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with P2A-EGFP

   Depositor

   Keith Joung

   Insert

   Human codon optimized pCAG BE4max with P2A-EGFP
   

   Use

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   D10A (SpCas9)

   Promoter

   CAG

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. lenti-UCOE-dCas9-BFP-Zim3-KRAB

   Plasmid

   #196716

   Purpose

   CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3

   Depositor

   Bernhard Schmierer

   Insert

   dCas9-TagBFP-KRAB(ZIM3)
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mutation

   D10A, N863A

   Promoter

   EF1a

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. TLR4

   Plasmid

   #20863

   Depositor

   Jie-Oh Lee

   Article

   Park et al Nature. 2009 Mar 1. ():.

   Insert

   Toll-Like Receptor 4 (TLR4 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Contains ectodomain (amino acids 27–631)

   Promoter

   Available since

   May 20, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT2/SVNeo

    Plasmid

    #26553

    Depositor

    Perry Hackett

    Article

    Cui et al J Mol Biol. 2002 May 17. 318(5):1221-35.

    Insert

    pT2/SVNeo
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    added the Rmut(80, 87, d101)

    Promoter

    Available since

    March 14, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRDB_092

    Plasmid

    #231110

    Purpose

    C>T base editor

    Depositor

    John Doench, David Root

    Insert

    TadA-CDd-nCas9-SpG-UGI
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    D10A

    Promoter

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDONR221-appa

    Plasmid

    #192745

    Purpose

    Gateway entry vector encoding zebrafish appa

    Depositor

    Summer Thyme

    Insert

    appa (appa Zebrafish)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    S47N

    Promoter

    None

    Available since

    Nov. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRDAX-EMMI-035 Lamp1(D-G)//Esp3I

    Plasmid

    #130237

    Purpose

    EMMA Kit # 1000000119 extension

    Depositor

    Gary Lewin

    Insert

    Lamp1 (Lamp1 Mouse)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHH0103 PDZK10 WW domain

    Plasmid

    #104285

    Purpose

    Bacterial expression of WW domain from PDZK10

    Depositor

    Marius Sudol, Sachdev Sidhu

    Insert

    PDZK10 WW domain (FRMPD4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. TDRD7 (3RCO)

    Plasmid

    #32051

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    TDRD7 (TDRD7 Human)
    

    Use

    Tags

    His (with TEV cleavage)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 21, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

16. EPB41L3A-c000

    Plasmid

    #175458

    Purpose

    Protein expression in bacterial cells. Entire CDS, M1-D1087.

    Depositor

    Opher Gileadi

    Insert

    EPB41L3 (EPB41L3 Human)
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. GST-RH▲-MNase

    Plasmid

    #136292

    Purpose

    Expression of catalytic inactive RNase H fused to micrococcal nuclease

    Depositor

    Kavitha Sarma

    Article

    Yan et al Cell Rep. 2019 Oct 29;29(5):1369-1380.e5

    Insert

    RNase HI-D10R-E48R and MNase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCSC-SP-PW-Nep (aka: pBOB-NEP)

    Plasmid

    #12338

    Depositor

    Inder Verma

    Article

    Marr et al J Mol Neurosci. 2004 . 22(1-2):5-11.

    Insert

    NEP (MME Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMOD3-mClover-nat

    Plasmid

    #120328

    Purpose

    Bacterial mClover promoter trap

    Depositor

    James Kirby

    Article

    Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

    Insert

    mClover
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D103N (see comments below)

    Promoter

    (None)

    Available since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBRC1

    Plasmid

    #183064

    Purpose

    Base editing plasmid for Pseudomonas chlororaphis

    Depositor

    Xuehong Zhang

    Article

    Yue et al Microb Biotechnol. 2022 Sep;15(9):2324-2

    Insert

    eSpCas9ppD10A
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge