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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,493 results for: D10

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Showing: 941 - 960 of 2493 results

  1. pX330-Flag-deSpCas9

    Plasmid
    #92114
    Purpose
    Expression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dead/inactive eSpCas9 with FLAG tag
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A, K848A, K1003A, R1060A
    Promoter
    Cbh
    Available since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92114-plasmid-map-sequence-id-197615

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  3. pCR1056

    Plasmid
    #71824
    Purpose
    Express Streptococcus pyogenes dCas9 (D10A, H840A) carrying two C-terminal SV40 NLS
    Depositor
    Jacob Corn
    Publication
    Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    T7pr_6xHis-MBP-TEV-SPydCas9 (D10A,H840A)-2xNLS
    Use
    CRISPR
    Available since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    71824-plasmid-map-sequence-id-131017

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  5. pYPQ262m

    Plasmid
    #161522
    Purpose
    Gateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE7.10 mediated A-G base editing
    Depositor
    Yiping Qi
    Publication
    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41
    Insert
    TadA(wt)-TadA(7.10)-zSpCas9(D10A)
    Use
    CRISPR; Gateway compatible tada(wt)-tada(7.10)-zs…
    Expression
    Plant
    Available since
    Feb. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    161522-plasmid-map-sequence-id-325551

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  9. ABE-NW-NL

    Plasmid
    #240621
    Purpose
    Adenine base editor with narrowed editing window
    Depositor
    Tingting Jiang
    Publication
    Valdez et al Nat Commun. 2025 Aug 30;16(1):8115. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TadA8e(N108Q,M151C,Q154Y)-nCas9 (D10A)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    TadA8e(N108Q,M151C,Q154Y), Cas9(D10A)
    Available since
    July 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    240621-plasmid-map-sequence-id-480853

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  10. pCMV-T7-ABE8.20m-nSpG-P2A-EGFP (KAC1160)

    Plasmid
    #185916
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ABE8.20m-nSpG-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185916-plasmid-map-sequence-id-367354

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  14. ABE-RA3.0

    Plasmid
    #208301
    Purpose
    Expresses ABE-RA3.0 in mammalian cells
    Depositor
    Weixin Tang
    Publication
    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TadA-RA3.0-SpCas9 D10A
    Expression
    Mammalian
    Mutation
    E27D, P48A, R51H, I76F, D108G, K110R, H122R, M126…
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    208301-plasmid-map-sequence-id-410368

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  15. pMOD_A1810

    Plasmid
    #91040
    Purpose
    Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_dead (D10A + H840A), Terminator: HSP
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Csy4-P2A-TaCas9_dead (D10A + H840A)
    Use
    CRISPR
    Mutation
    D10A, H840A
    Promoter
    ZmUbi
    Available since
    July 10, 2017
    Availability
    Academic Institutions and Nonprofits only

  17. pLenti PGK mKate2-OGT D554N (Puro)

    Plasmid
    #154292
    Purpose
    Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, D554N variant, no Ser/Thr glycosylation)
    Depositor
    Suzanne Walker
    Publication
    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11
    Insert
    O-GlcNAc Transferase (OGT Human)
    Use
    Lentiviral
    Tags
    2x FLAG and mKate2
    Expression
    Mammalian
    Mutation
    D554N mutation (Ser/Thr glycosylation-incompetent)
    Promoter
    PGK
    Available since
    May 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    154292-plasmid-map-sequence-id-332434

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  18. LADL Anchor

    Plasmid
    #127664
    Purpose
    Encodes the LADL Anchor (dCas9-CIBN fusion protein) and puromycin resistance both expressed from the EF1a promoter
    Depositor
    Jennifer Phillips-Cremins
    Publication
    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-CIBN-2A-Puro
    Tags
    3XFLAG
    Expression
    Mammalian
    Mutation
    Cas9 (D10A,H840A) mutant
    Promoter
    EF1a
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127664-plasmid-map-sequence-id-247037

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  19. p3s-ABE7.10

    Plasmid
    #113128
    Purpose
    expressing ABE7.10 (TadA-TadA*7.10-nCas9-NLS-HA), human codon optimized
    Depositor
    Jin-Soo Kim
    Publication
    Ryu et al Nat Biotechnol. 2018 Jul;36(6):536-539.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ABE 7.10
    Tags
    HA epitope and SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    113128-plasmid-map-sequence-id-216920

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Showing: 941 - 960 of 2493 results