We narrowed to 4,158 results for: SPL

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AAVS1-A2UCOE-EF1a-mCR1-F2A-neo-AAVS1

Plasmid

#205447

Purpose

human AAVS1-targeting plasmid for expression of mouse CR1

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

mCR1

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pHBS1501 [IBB-GFP-mCherry3E]-[BFP-TDP43 deltaRRM]

Plasmid

#133325

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

Deletion of RRM in full-length TDP43

Available Since

Nov. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLX317-RBFOX1

Plasmid

#115442

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

RBFOX1 (RBFOX1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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AAVS1-iSAM

Plasmid

#211495

Purpose

AAVS1 targeting vector containing an all-in-one DOX-inducible system for CRISPRa

Depositor

Antonella Fidanza, Lesley Forrester

Article

Petazzi et al Elife. 2024 Dec 23;13:RP94884. doi: 10.7

Inserts

UniSAM insert
TET-ON
Neo Resistance
Insulator

Use

CRISPR and Synthetic Biology

Tags

T2A-mCherry Tag

Expression

Mammalian

Promoter

EF1a, NA, Splicing acceptor, and TREGV promoter

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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modified pcAT7-Glo1

Plasmid

#160996

Purpose

Splicing reporter backbone

Depositor

Brenton Graveley

Article

Adamson et al Genome Biol. 2018 Jun 1;19(1):71. doi: 1

Insert

hemoglobin subunit beta (HBB Human)

Expression

Mammalian

Mutation

Repeated intron 1 with 40 nucelotide deletion

Promoter

CAG

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pSicoR-Ef1a-mCh-Puro-GFPi

Plasmid

#31848

Depositor

Bruce Conklin

Article

Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

Insert

GFP RNAi

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

U6 for shRNA; Ef1alpha for mCherry-puromycin

Available Since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pAC1812_pCR8-gCasRx-CUG (control gRNA)

Plasmid

#118645

Purpose

Transient transfection; Expressed Control (CUG repeat) CasRx gRNA; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

gCasRx-CUG

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2-hSeparase-wt

Plasmid

#33016

Depositor

Marc Kirschner

Article

Stemmann et al Cell. 2001 Dec 14;107(6):715-26.

Insert

Separase (ESPL1 Human)

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
pYZ158

Plasmid

#98414

Purpose

Pombe Expression Vector - nmt1 promoter with Sp.leu1 marker

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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mSprouty4 EST

Plasmid

#22093

Depositor

Gail Martin

Article

Minowada et al Development. 1999 Oct . 126(20):4465-75.

Insert

Sprouty 4 (Spry4 Mouse)

Use

Bacterial

Available Since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only
pAAVS1-Puro-CAG-mNG2(1-10)

Plasmid

#206042

Purpose

Repair template for the integration of a mNG2(1-10) expression cassette into the AAVS1 locus in human cells.

Depositor

Alisa Piekny

Article

Husser et al Elife. 2024 Apr 23;12:RP92819. doi: 10.7

Insert

AAVS1 homology arms with SA-P2A-Puro marker and CAG-mNG2(1-10) expression cassette

Use

CRISPR and Synthetic Biology; Donor template

Expression

Mammalian

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-hPMCA2x/b

Plasmid

#47585

Depositor

Emanuel Strehler

Article

Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

Insert

pMCA2x/b (ATP2B2 Human)

Tags

EGFP-HA-His

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only
pGLOW77

Plasmid

#177338

Purpose

C. elegans mNeonGreen co-injection marker

Depositor

Ryan Doonan

Article

Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

Insert

Pmyo-2::mNeonGreen

Tags

mNeonGreen

Expression

Worm

Promoter

myo-2

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pMMRSF17

Plasmid

#20178

Depositor

Hideo Iwai

Article

Muona et al Chembiochem. 2008 Dec 15. 9(18):2958-61.

Insert

C-CrkII nSH3 (CRK Human)

Tags

NpuNdelC16 and Smt3

Expression

Bacterial

Available Since

Aug. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pNL(CMV)GLuc/CMV/WPREdU3

Plasmid

#136999

Purpose

Reporter vector encoding Gaussia luciferase

Depositor

Jakob Reiser

Article

Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13

Insert

Gaussia luciferase

Use

Lentiviral

Promoter

CMV

Available Since

Feb. 7, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pUC19-P70a-3WJdB-T

Plasmid

#87311

Purpose

Expresses the fluorescence-activating Three-way Junction dimeric Broccoli (3WJdB) aptamer under the control of the E. coli sigma 70 promoter.

Depositor

Donald Burke

Article

Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

Insert

Three-way Junction dimeric Broccoli aptamer

Use

Synthetic Biology

Expression

Bacterial

Promoter

P70a

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO5d.SFFV.eGFP-miR30n

Plasmid

#90333

Purpose

miR30 based shMir knockdown

Depositor

Dirk Heckl

Article

Schwarzer et al Nat Commun. 2017 Aug 9;8(1):218. doi: 10

Inserts

eGFP
na
SFFV
miR30n

Use

Lentiviral

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pJYDC3

Plasmid

#162460

Purpose

Yeast display plasmid for expression of C-terminal fusions with Aga2p yeast agglutinin. The reporter DnbALFA was fused to the N-terminus of Aga2p.

Depositor

Gideon Schreiber

Article

Zahradník et al bioRxiv Preprint 2020

Type

Empty backbone

Use

Shuttle vector bacteria/yeast

Tags

DnbALFA designed ALFA nanobody, HA tag, HDEL sequ…

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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CAG::Gdnf

Plasmid

#78209

Purpose

piggybac vector for mouse Gdnf expression

Depositor

Ivo Lieberam

Article

Bryson et al Science. 2014 Apr 4;344(6179):94-7. doi:

Insert

Gdnf (Gdnf Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pU6-neo-TET

Plasmid

#51286

Purpose

expresses U6 RNA, using its own pol III enhancer sequence and tagged with the self splicing intron neo cassette

Depositor

Astrid Roy-Engel

Article

Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

Insert

U6 (RNU6-1 Human)

Tags

neoTET cassette with a tetrahymena self-splicing …

Expression

Mammalian

Promoter

U6 upstream enhancer sequence

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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