Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 3,871 results for: SPL

Showing: 941 - 960 of 3871 results

  2. pSLQ8666 pHR-RRM2p-BFP-T2A-N term (1-406) dLbCpf1-EE12RR345L-EFS-Zeo-WPRE

    Plasmid
    #140228
    Purpose
    RRM2p-driven expression of BFP and N terminal dCpf1 (Cas12a) 406-split half with leucine zipper. Constitutive EFS-driven zeocin resistance.
    Depositor
    Stanley Qi
    Article
    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2
    Insert
    dLbCpf1 (Cas12a) N terminal half (406) with zipper
    Use
    Lentiviral
    Tags
    BFP
    Expression
    Mammalian
    Mutation
    Promoter
    RRM2 endogenous promoter
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    140228-plasmid-map-sequence-id-291990

    Enlarge

  6. AP4mS_PPVs_P3_cLuc

    Plasmid
    #119300
    Purpose
    Expresses C-terminus of split luciferase fused to P3 coil, PPVs cleavable linker and autoinhibitory coil AP4/for split protease-cleavable orthogonal Coiled-coil (SPOC) logic
    Depositor
    Roman Jerala
    Article
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Insert
    split cLuc fused to P3coil-PPV cleavage site and autoinhibitory coil AP4mS
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    119300-plasmid-map-sequence-id-232041

    Enlarge

  7. nDHFR173-gs10-NLS-VPR

    Plasmid
    #188262
    Purpose
    Plasmid ensures constitutive expression of the N-terminal fragment of split DHFR (aa 174-186), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus
    Depositor
    Roman Jerala
    Article
    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038
    Insert
    nDHFR173 (DHFR Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    188262-plasmid-map-sequence-id-374570

    Enlarge

  9. pSLQ8629 pHR-HREYBTATA-RR12EE345L-C term (406) dLbCpf1-2XNLS-miniVPR-mCherry-EFS-Puro-WPRE

    Plasmid
    #140227
    Purpose
    Hypoxia-inducible expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to miniVPR. Constitutive EFS-driven puromycin resistance.
    Depositor
    Stanley Qi
    Article
    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2
    Insert
    zipper with dLbCpf1 (Cas12a) C terminal half (406) fused to miniVPR
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    HRE (Hypoxia inducible)
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    140227-plasmid-map-sequence-id-291996

    Enlarge

  12. pSF3-Flag-Cbir-Cdc25C S216A_Myc-Nbir-14-3-3e

    Plasmid
    #90011
    Purpose
    Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C S216A and N-terminally tagged NBir[2-256]-14-3-3e
    Depositor
    Julien Béthune
    Article
    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.
    Inserts
    CBirA*-Cdc25C S216A
    NBirA*-14-3-3e
    Use
    Retroviral; Flp/frt
    Tags
    FLAG and Myc
    Expression
    Mammalian
    Mutation
    Ser 216 to Ala
    Promoter
    CMV
    Available since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    90011-plasmid-map-sequence-id-201243

    Enlarge

  13. exon L-firefly

    Plasmid
    #81006
    Purpose
    luciferase based mini-gene to report splicing at exon 25 in Drosophila parayltic
    Depositor
    Richard Baines
    Article
    Lin et al Brain. 2015 Apr;138(Pt 4):891-901. doi:
    Insert
    paralytic (para Fly)
    Use
    Tags
    firefly luciferase
    Expression
    Insect
    Mutation
    spanning exon 24 to exon 26, termination codon in…
    Promoter
    P-AC5 (Actin 5C)
    Available since
    Aug. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    81006-plasmid-map-sequence-id-158526

    Enlarge

  20. exon K-renilla

    Plasmid
    #81007
    Purpose
    luciferase based mini-gene to report splicing at exon 25 in Drosophila parayltic
    Depositor
    Richard Baines
    Article
    Lin et al Brain. 2015 Apr;138(Pt 4):891-901. doi:
    Insert
    paralytic (para Fly)
    Use
    Tags
    Renilla luciferase
    Expression
    Insect
    Mutation
    spanning exon 24 to exon 26, termination codon in…
    Promoter
    P-AC5 (Actin 5C)
    Available since
    Aug. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    81007-plasmid-map-sequence-id-158524

    Enlarge

Showing: 941 - 960 of 3871 results