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Human J-chain

Plasmid

#158215

Purpose

Express the human J-chain

Depositor

Junyu Xiao

Article

Li et al Science. 2020 Feb 28;367(6481):1014-1017

Insert

JCHAIN (JCHAIN Human)

Tags

8×his

Expression

Mammalian

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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SMA-Cherry

Plasmid

#110209

Purpose

Mapping of mesenchymal progenitor cells.

Depositor

David Rowe

Article

Grcevic et al Stem Cells. 2012 Feb;30(2):187-96. doi:

Insert

mCherry

Tags

None

Expression

Mammalian

Promoter

Smooth muscle alpha-actin and first intron

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ132B2.0

Plasmid

#99888

Purpose

Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU3

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-PE2-SpG

Plasmid

#159978

Purpose

Prime editing in mammalian cells

Depositor

Yongsub Kim

Article

Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

Insert

PE2-SpG

Tags

BpSV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCI-MEG3

Plasmid

#44727

Depositor

Anne Klibanski

Article

Zhou et al J Biol Chem. 2007 Aug 24;282(34):24731-4

Insert

Maternally Expressed Gene 3 (MEG3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2013

Availability

Academic Institutions and Nonprofits only

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pYPQ131B2.0

Plasmid

#99885

Purpose

Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU3

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pBad-LgBiT-PhoCl1-SmBiT-MBP

Plasmid

#164034

Purpose

luciferase-based PhoCl screening construct

Depositor

Robert Campbell

Article

Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

Insert

LgBiT-PhoCl1-SmBiT-MBP

Tags

His tag, T7 tag, Xpress Tag

Expression

Bacterial

Promoter

araBad promotor

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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double cut NH-donor

Plasmid

#83576

Purpose

eGFP reporter donor containing double SA cutting sites for NHEJ knock-in

Depositor

Bo Feng

Article

He et al Nucleic Acids Res. 2016 May 19;44(9):e85

Insert

eGFP

Use

Nhej donor

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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LRG2.1_Neo

Plasmid

#125593

Purpose

Lentiviral expression of sgRNA with GFP and neomycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter for sgRNA expression and EFS promoter…

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pAG001 His6-TwinStrep-SUMO-AapCas12b

Plasmid

#153162

Purpose

Bacterial expression plasmid for AapCas12b

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Joung et al medRxiv. 2020 May 8. doi: 10.1101/2020.0

Insert

AapCas12b

Expression

Bacterial

Available Since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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pVEe

Plasmid

#234928

Purpose

All-in-one plant virus expression vector for virus-induced genome editing (VIGE).

Depositor

Guoli Song

Article

Hao et al Plant Commun. 2025 May 19:101379. doi: 1

Type

Empty backbone

Expression

Plant

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCambia3301-AtU6-MAR1

Plasmid

#210757

Purpose

Mutagenesis of the Arabidopsis MAR1 gene for efficient screening.

Depositor

Daisuke Miki

Article

Cheng et al Sci Rep. 2024 Dec 28;14(1):30791. doi: 1

Insert

AtU6-26 pro::MAR1 sgRNA

Use

CRISPR

Expression

Plant

Promoter

AtU6-26

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET15bpATnp

Plasmid

#121137

Purpose

This vector encode a His6-tagged proteinA-Tnp(hyperactive form) fusion protein.

Depositor

Keji Zhao

Article

Carter et al Nat Commun. 2019 Aug 20;10(1):3747. doi:

Insert

Protein A-Tnp

Tags

his

Expression

Bacterial

Promoter

T7

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-TRAIL

Plasmid

#10953

Depositor

Bingliang Fang

Article

Kagawa et al Cancer Res. 2001 Apr 15. 61(8):3330-8.

Insert

Tumor necrosis factor alpha related apoptosis inducing ligand (TNFSF10 Human)

Tags

GFP

Expression

Mammalian

Mutation

NA

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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LRG2.1_Puro

Plasmid

#125594

Purpose

Lentiviral expression of sgRNA with GFP and puromycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter for sgRNA expression and EFS promoter…

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG_smFP FLAG

Plasmid

#59756

Purpose

encodes multiple copies of an epitope tag FLAG

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

smFP _FLAG

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pET-11b_PcrA_M6_H93A

Plasmid

#194156

Purpose

Engineered PcrA_M6 helicase to support isothermal amplification method SHARP

Depositor

Taekjip Ha

Article

Gavrilov et al Nat Commun. 2022 Oct 23;13(1):6312. doi:

Insert

Engineered helicase PcrA_M6

Tags

6x His tag and FLAG tag

Expression

Bacterial

Mutation

H93A, C96A, N187C, C247A, L384V, L409C

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLOVE-miR302/367

Plasmid

#31310

Depositor

Ed Morrisey

Article

Anokye-Danso et al Cell Stem Cell. 2011 Apr 8. 8(4):376-88.

Insert

mmu-miR302/367

Use

Lentiviral

Expression

Mammalian

Mutation

None

Available Since

Oct. 25, 2011

Availability

Academic Institutions and Nonprofits only
pBMNb-cALFA

Plasmid

#159991

Purpose

For the protein expression with ALFA tag at C-terminus in mammalian cells

Depositor

Motoyuki Hattori

Article

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Type

Empty backbone

Tags

HRV3C protease cleavage site-ALFA tag-twin Strep …

Expression

Mammalian

Mutation

CMV promoter, WPRE

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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QPM-sgR (pTaU3)

Plasmid

#140448

Purpose

For sgRNA expression in monocotyledons protoplasts

Depositor

Caixia Gao

Article

Lin et al Nat Biotechnol. 2020 May;38(5):582-585.

Insert

TaU3-sgRNA

Use

CRISPR

Expression

Plant

Promoter

TaU3

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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