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pLJC2-UMPS-3xFLAG

Plasmid

#87975

Purpose

3rd generation lentiviral vector for Expression of UMPS in mammalian cells

Depositor

David Sabatini

Article

Cantor et al Cell. 2017 Apr 6;169(2):258-272.e17. doi

Insert

UMPS (UMPS Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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pMAL_dstβL

Plasmid

#73805

Purpose

Plasmid for selection for insertion and homodimerization

Depositor

Sarel Fleishman

Article

Elazar et al Elife. 2016 Jan 29;5. pii: e12125. doi:

Inserts

CAT Marker
ToxR
beta lactamase
spectomycin resistance
CLS TM domain

Tags

Part Of the dstbl chiemra, Part of the dsTbL chim…

Expression

Bacterial

Promoter

NaN and ToxR promotor

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
pLenti-hSyn-Gβγ-BERKY1

Plasmid

#158429

Purpose

BRET biosensor for detection of free Gβγ in lentiviral vector pLenti-hSyn

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-YFP-GRK3ct

Use

Lentiviral

Expression

Mammalian

Promoter

hSynapsin

Available Since

Sept. 17, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-synP-FLEX-splitTVA-EGFP

Plasmid

#52474

Purpose

Expresses splitTVA-EGFP

Depositor

Ian Wickersham

Article

Kohara et al Nat Neurosci. 2014 Feb;17(2):269-79. doi

Insert

splitTVA-P2A-EGFP

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

Synapsin-1

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

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RhoC FLARE.sc mCer, mVenus - CA

Plasmid

#65424

Purpose

RhoC CA biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC Q63L

Depositor

Klaus Hahn

Article

Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

Insert

RBD, mCerulean, mVenus, RhoC

Expression

Bacterial, Insect, and Mamm…

Mutation

T153M in mVenus, Q63L in RhoC

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only
RhoC FLARE.sc mCer, mVenus - DN

Plasmid

#65423

Purpose

RhoC DN biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC T19N

Depositor

Klaus Hahn

Article

Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

Insert

RBD, mCerulean, mVenus, RhoC

Expression

Bacterial, Insect, and Mamm…

Mutation

T153M in mVenus, T19N in RhoC

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only
VinTS-tCRMod-GGSGGS5

Plasmid

#111763

Purpose

Expresses vinculin tension sensor comprised of Clover-(GGSGGS)5-mRuby2 module

Depositor

Brenton Hoffman

Article

LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

Insert

Clover-mRuby2-based vinculin tension sensor with (GGSGGS)5 extensible domain

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAG306-GPD-VMA1 chr I

Plasmid

#41896

Purpose

Yeast expression of VMA1.

Depositor

Dan Gottschling

Article

Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

Insert

VMA1 (VMA1 Budding Yeast)

Expression

Yeast

Mutation

Contains silent mutation at 342A-G and L87S mutat…

Promoter

GPD

Available Since

April 17, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-H2A-Akt-STOPS

Plasmid

#175010

Purpose

Akt-STOPS targeted to the nucleus via histone H2A.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

H2A-mCherry-Akt-STOPS

Tags

Histone H2A and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCPP5951

Plasmid

#128719

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Kvitko et al PLoS Pathog. 2009 Apr;5(4):e1000388. doi

Insert

hopR1

Expression

Bacterial

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pMIG-N19RhoA

Plasmid

#14120

Depositor

Richard Hynes

Article

Clark et al Nature. 2000 Aug 3. 406(6795):532-5.

Insert

N19 Human RhoA (RHOA Human)

Use

Retroviral

Tags

Myc

Mutation

Site directed mutagenesis of Thr to Asn at Codon …

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pHW450

Plasmid

#198831

Purpose

Voltage-sensitive fluorescent protein.

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::VSFP-Butterfly-1-2::let-858 3'UTR

Expression

Worm

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn FLEx-loxp hM4Di-mCherry

Plasmid

#161581

Purpose

Expression of hM4Di-mCherry in a cre-dependent fashion

Depositor

Kevin Beier, Robert Malenka

Article

Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

Insert

hM4Di-mCherry

Use

AAV

Mutation

NA

Promoter

hSyn

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCOLA-Spec-yibDp-Erv1p

Plasmid

#212094

Purpose

Expresses sulfydryl oxidase (Erv1p) after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

Sulfydryl oxidase (ERV1 Budding Yeast)

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-6020nes

Plasmid

#108656

Purpose

A JNK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

Depositor

Michiyuki Matsuda

Article

Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

Insert

hyBRET-JNK-EV

Tags

XFP

Expression

Mammalian

Available Since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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N-terminal Halo-XLF

Plasmid

#207546

Purpose

Homologous recombination donor to insert halo tag at the N terminal of the endogenous XLF locus.

Depositor

Jens Schmidt

Article

Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

Insert

HaloTag with internal PuroR cassette flanked by human Lig4 locus sequences

Tags

HaloTag

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AktAR-Kras

Plasmid

#123357

Purpose

FRET-based biosensor for monitoring Akt activity in non-raft plasma membrane regions.

Depositor

Jin Zhang

Article

Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

Insert

AktAR-Kras

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-Akt-STOPS

Plasmid

#175009

Purpose

Plasma membrane-targeted Akt-STOPS.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

Lyn-mCherry-Akt-STOPS

Tags

N-terminal targeting sequence from Lyn kinase and…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pDragon-empty

Plasmid

#155018

Purpose

Empty Tigre-Dragon vector. Genes of interest can be cloned using Mlu-I and SnaB-I. For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA.

Depositor

Alberto Rosello-Diez

Article

Ahmadzadeh et al Development. 2020 May 28;147(10). pii: d

Type

Empty backbone

Use

Mouse Targeting

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pJSK418

Plasmid

#205131

Purpose

Expression of GFP-2xFYVE in Dictyostelium

Depositor

Jason King

Article

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

2xFYVE (from human Hrs) (HGS Synthetic)

Use

Dictyostelium expression

Tags

EGFP

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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