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BCL6-GFP Y58A in Artichoke

Plasmid

#169936

Purpose

Overexpression of BCL6-GFP Y58A mutant fusion

Depositor

Benjamin Ebert

Article

Slabicki et al Nature. 2020 Dec;588(7836):164-168. doi:

Insert

BCL6 Y58A (BCL6 Human)

Use

Lentiviral

Tags

GFP

Mutation

160R>C

Available Since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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BCL6-GFP R28A in Artichoke

Plasmid

#169933

Purpose

Overexpression of BCL6-GFP R28A mutant fusion

Depositor

Benjamin Ebert

Article

Slabicki et al Nature. 2020 Dec;588(7836):164-168. doi:

Insert

BCL6 R28A (BCL6 Human)

Use

Lentiviral

Tags

GFP

Mutation

160R>C

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pPICZc-Ub-R-HsACTB*-thymosinB-8His

Plasmid

#111149

Purpose

Expresses arginylated human beta-actin fused with thymosin beta and a His tag.

Depositor

Mohan Balasubramanian

Article

Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827

Insert

ACTB (ACTB Human)

Use

Pichia pastoris integration plasmid

Tags

Thymosin beta and a His-tag

Expression

Yeast

Mutation

N-terminal arginylation

Promoter

AOX1

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pQCXIZ GFP PODXL delta PBM + FBM

Plasmid

#202416

Purpose

Expression of GFP-tagged PODXL PDZ-binding and FERM-binding motif mutation

Depositor

David Bryant

Article

Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

Insert

PODXL (PODXL Human)

Use

Lentiviral

Tags

GFP

Mutation

Lacking DTHL, and H477/R479/S481>AAA mutation

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv1-sgSHMT1-G3

Plasmid

#83903

Purpose

stable knockout

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

Serine hydroxymethyltransferase 1 (SHMT1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pQCXIZ GFP PODXL FKBP 4K>R

Plasmid

#202421

Purpose

Expression of ubiquitination-deficient GFP-tagged PODXL with FKBP-rapamycin binding (FRB) dimerization domain

Depositor

David Bryant

Article

Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

Insert

PODXL (PODXL Human)

Use

Lentiviral

Tags

FKBP and GFP

Mutation

K431/525/526/547R

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pIGF1_P2 Promoter-FLuc

Plasmid

#154267

Purpose

Firefly luciferase reporter containing the downstream Human IGF-1 P2 Promoter.

Depositor

Antonio Amelio

Article

Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

Insert

IGF-1 P2 Promoter (IGF1 Human)

Expression

Mammalian

Promoter

Human IGF-1 P2 Promoter

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACE2v6-Fc(IgA1)

Plasmid

#145161

Purpose

Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 6

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Tags

Fc region of human IgA1

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available Since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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PET.SUMO eIF4G1 +MIC 1-200

Plasmid

#158792

Purpose

Bacterial expression vector for the expression of N-terminal 6xHIS-SUMO-tagged codon optimized eIF4G1 amino acids 1-200 isoform including the microexon.

Depositor

Benjamin Blencowe

Article

Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

Insert

eIF4G1 (with microexon) codon optimized

Tags

6xHIS and SUMO

Expression

Bacterial

Mutation

amino acids 1-200

Promoter

T7

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pRRL-MND-hCard11 R72L-T2A-GFP

Plasmid

#163784

Purpose

Expression of R72L CARD domain variant

Depositor

Richard James

Article

Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

Insert

CARD11 (CARD11 Human)

Use

Lentiviral

Mutation

R72L

Promoter

MND

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-LucAL

Plasmid

#174049

Purpose

Expresses mALG8 (ITYTWTRL) and mLAMA4 (VGFNFRTL) antigens as a fusion to luciferase and Cre recombinase

Depositor

Tyler Jacks

Article

Burger et al Cell. 2021 Sep 16;184(19):4996-5014.e26.

Insert

LucAL

Use

Cre/Lox, Lentiviral, and Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

ALG8 alanine 506 to threonine; LAMA4 glycine 1254…

Promoter

Human Ubiquitin C

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pU6-crRNA(SOD1)

Plasmid

#109321

Purpose

AAV vector expressing crRNA for AsCpf1 (RR variant) targeting human SOD1

Depositor

Feng Zhang

Article

Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

Insert

mCherry-KASH (SOD1 Human)

Use

AAV

Promoter

hSyn1

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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ACTIN-Cxcl5-PGK-Cre

Plasmid

#110278

Purpose

Bicistronic lentivirus expressing Cxcl5 and Cre

Depositor

Trudy Oliver

Article

Mollaoglu et al Immunity. 2018 Oct 16;49(4):764-779.e9.

Inserts

chemokine (C-X-C motif) ligand 5 (Cxcl5 Mouse)
cyclization recombinase

Use

Cre/Lox and Lentiviral

Tags

No fusion genes

Expression

Mammalian

Mutation

Wild type

Promoter

B-actin and Pgk

Available Since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only
pDONR221-HES3

Plasmid

#101411

Purpose

Donor Vector containing HES3 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

HES3 (HES3 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5 rtTA BirA eIF4G

Plasmid

#158789

Purpose

pcDNA5 based transfection plasmid for the exogenous expression of N-terminal BirA-Flag-tagged eIF4G1 lacking the microexon for generation of N2A FlpIn cell lines for BioID

Depositor

Benjamin Blencowe

Article

Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

Insert

eIF4G1

Tags

3xFlag and BirA*

Expression

Mammalian

Promoter

miniCMV

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pET11d-FHis-DND1

Plasmid

#70083

Purpose

Bacterial expression of FLAG-and 6xHis-tagged human DND1 protein. Codons are optimized for bacteria expression.

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Insert

Dnd1 (DND1 Human)

Tags

6xHIS and FLAG

Expression

Bacterial

Mutation

Codon optimized for bacterial expression

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pET11d-FHis-DND1-Y102A

Plasmid

#70082

Purpose

Bacterial expression of FLAG-and 6xHis-tagged human DND1 protein with defective RRM (Y102A). Codons are optimized for bacteria expression.

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Insert

Dnd1 (DND1 Human)

Tags

6xHIS and FLAG

Expression

Bacterial

Mutation

Y102A. Codon optimized for bacterial expression

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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LTR-[Bxb1(attB)-spacer-BceINT(attB)-spacer-Pa01(attB)-spacer-SacINT(attB)-pEF1a-Puro-LTR (DRR724)

Plasmid

#248231

Purpose

Lentiviral plasmid containing Bxb1, BceINT, Pa01, and SacINT attB target sites

Depositor

Benjamin Kleinstiver

Article

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

LTR-[Bxb1(attB)-spacer-BceINT(attB)-spacer-Pa01(attB)-spacer-SacINT(attB)-pEF1a-Puro-LTR

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

None

Promoter

NA

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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pGL4.10 HIF1a prom Chr5-LG4

Plasmid

#246714

Purpose

To investigate the influence of an LG4 enhancer (chr5 LG4) on HIF1a promoter activity (measured by luciferase expression)

Depositor

Glen Borchert

Article

DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Inserts

HIF1a Promoter (HIF1A Human)
chr5 LG4 enhancer

Use

Luciferase

Mutation

WT

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pGL4.10 EXOC3prom Chr5-LG4

Plasmid

#246712

Purpose

To investigate the influence of an LG4 enhancer (chr5 LG4) on EXOC3 promoter activity (measured by luciferase expression)

Depositor

Glen Borchert

Article

DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Inserts

EXOC3 Promoter (EXOC3 Human)
chr5 LG4 enhancer

Use

Luciferase

Mutation

WT

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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