Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 14,263 results for: crispr grnas

Clear keywords
Showing: 9721 - 9740 of 14263 results

  1. lentiSAMv2

    Plasmid
    #75112
    Purpose
    lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available Since
    May 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    75112-plasmid-map-sequence-id-305764

    Enlarge

  4. pCMU-4

    Plasmid
    #172353
    Purpose
    CRISPR gene editing in Streptomyces, codon-optimized Cas9 behind theophylline riboswitch and D4 promoter, with custom gRNA
    Depositor
    Eriko Takano
    Article
    Ye et al J Ind Microbiol Biotechnol. 2020 May;47(
    Insert
    D4-TheoR-cas9
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    D4
    Available Since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    172353-plasmid-map-sequence-id-350007

    Enlarge

  5. OgeuIscB CTG RNA

    Plasmid
    #222865
    Purpose
    This plasmid codes for the guide of the OgeuIscB with a CTG target
    Depositor
    Vincent Dion
    Article
    Mangin et al bioRxiv 2023.10.24.563270
    Insert
    OgeuIscB omega RNA with a (CTG)6 target sequence
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Human U6
    Available Since
    Oct. 17, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    222865-plasmid-map-sequence-id-449444

    Enlarge

  6. OgeuIscB GCT RNA

    Plasmid
    #222862
    Purpose
    This plasmid codes for the guide of the OgeuIscB with a GCT target
    Depositor
    Vincent Dion
    Article
    Mangin et al bioRxiv 2023.10.24.563270
    Insert
    OgeuIscB omega RNA with a (GCT)6 target sequence
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Human U6
    Available Since
    Oct. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    222862-plasmid-map-sequence-id-449454

    Enlarge

  7. OgeuIscB GCA RNA

    Plasmid
    #222864
    Purpose
    This plasmid codes for the guide of the OgeuIscB with a GCA target
    Depositor
    Vincent Dion
    Article
    Mangin et al bioRxiv 2023.10.24.563270
    Insert
    OgeuIscB omega RNA with a (GCA)6 target sequence
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Human U6
    Available Since
    Oct. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    222864-plasmid-map-sequence-id-449452

    Enlarge

  8. OgeuIscB TGC RNA

    Plasmid
    #222863
    Purpose
    This plasmid codes for the guide of the OgeuIscB with a TGC target
    Depositor
    Vincent Dion
    Article
    Mangin et al bioRxiv 2023.10.24.563270
    Insert
    OgeuIscB omega RNA with a (TGC)6 target sequence
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Human U6
    Available Since
    Oct. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    222863-plasmid-map-sequence-id-449446

    Enlarge

  9. OgeuIscB AGC RNA

    Plasmid
    #222866
    Purpose
    This plasmid codes for the guide of the OgeuIscB with a AGC target
    Depositor
    Vincent Dion
    Article
    Mangin et al bioRxiv 2023.10.24.563270
    Insert
    OgeuIscB omega RNA with a (AGC)6 target sequence
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Human U6
    Available Since
    Oct. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    222866-plasmid-map-sequence-id-449445

    Enlarge

  10. OgeuIscB CAG RNA

    Plasmid
    #222867
    Purpose
    This plasmid codes for the guide of the OgeuIscB with a CAG target
    Depositor
    Vincent Dion
    Article
    Mangin et al bioRxiv 2023.10.24.563270
    Insert
    OgeuIscB omega RNA with a (CAG)6 target sequence
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Human U6
    Available Since
    Oct. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    222867-plasmid-map-sequence-id-449443

    Enlarge

  11. pAIO-Ef1a-PE2-GFP:KCNQ2-C201R_pp

    Plasmid
    #185061
    Purpose
    Ef1a driven PE2 with pegRNA for editing C201R mutation (including silent PAM mutation) in KCNQ2. See Addgene plasmid #184445
    Depositor
    Bobby Koeleman
    Insert
    U6:pegRNA:scaffold:PBS+RT template
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Nov. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    185061-plasmid-map-sequence-id-376807

    Enlarge

  16. PX552

    Plasmid
    #60958
    Purpose
    pAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei.
    Depositor
    Feng Zhang
    Article
    Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103
    Inserts
    U6_(SpaI)_sgRNA
    Syn_EGFP-KASH
    Use
    AAV, CRISPR, and Mouse Targeting
    Expression
    Mammalian
    Promoter
    Synapsin and U6
    Available Since
    Nov. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    60958-plasmid-map-sequence-id-382829

    Enlarge

  17. pYPQAT33

    Plasmid
    #223405
    Purpose
    T-DNA vector for SpCas9-D10A based A-to-G base editing for dicot plants; NGG PAM; SpCas9D10A-ABE was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-ecTadA8e-SpCas9-D10A-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223405-plasmid-map-sequence-id-452221

    Enlarge

  18. pYPQAT31

    Plasmid
    #223403
    Purpose
    T-DNA vector for SpCas9-D10A based A-to-G base editing for dicot plants; NGG PAM; SpCas9D10A-ABE was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    AtUBQ10-ecTadA8e-SpCas9-D10A-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223403-plasmid-map-sequence-id-454164

    Enlarge

  19. pYPQAT57

    Plasmid
    #223429
    Purpose
    T-DNA vector for dSpCas9 mediated gene activation for dicot plants; NGG PAM; dSpCas9-Act3.0 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-dSpCas9-Act3.0-AtU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223429-plasmid-map-sequence-id-453502

    Enlarge

  20. pYPQAT58

    Plasmid
    #223430
    Purpose
    T-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-dSpCas9-Act3.0-OsU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223430-plasmid-map-sequence-id-454181

    Enlarge

Showing: 9721 - 9740 of 14263 results