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169,450 results

Showing: 9761 - 9780 of 169450 results

  1. pLenti GW V5 Eco/Dam hum LaminB1

    Plasmid
    #182671
    Purpose
    Eukaryotic expression vector with E.Coli Dam methylation fused to human Lamin B1. Used in DamID experiments to methylate DNA that interacts in the proximity of Lamin B1.
    Depositor
    Bas van Steensel
    Article
    Guelen et al Nature. 2008 Jun 12;453(7197):948-51. do
    Insert
    Lamin B1 (LMNB1 Human)
    Use
    Lentiviral
    Tags
    E. Coli Dam and V5
    Expression
    Mammalian
    Promoter
    HSP
    Available Since
    April 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    182671-plasmid-map-sequence-id-450317

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  8. pHIS-GB1-BP1

    Plasmid
    #133427
    Purpose
    Human BP1 fragment of SREBF1 with GB1 solubility tag in vector for in vitro expression, with T7 promoter.
    Depositor
    Gerhard Wagner
    Insert
    SREBF1 (SREBF1 Human)
    Expression
    Bacterial
    Mutation
    SREBP1a amino acids 17-42 fused to GB1 solubilit…
    Available Since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133427-plasmid-map-sequence-id-267726

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  13. pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (AAV PHP.eB)

    Viral Prep
    #20298-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (#20298). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA plasmid DNA. EF1a-driven, Cre-dependent, humanized channelrhodopsin H134R mutant fused to EYFP, for optogenetic activation. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    EF1a
    Tags
    EYFP (Cre-dependent)
    Available Since
    Sept. 8, 2020
    Availability
    Academic Institutions and Nonprofits only

  17. pHR-UCOE-SFFV-Zim3-dCas9-P2A-EGFP

    Plasmid
    #188899
    Purpose
    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element
    Depositor
    Marco Jost, Jonathan Weissman
    Article
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    Zim3-dCas9
    Use
    Lentiviral
    Tags
    HA-2xNLS, P2A-GFP, and Zim3 KRAB-NLS fusion
    Promoter
    SFFV
    Available Since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    188899-plasmid-map-sequence-id-374831

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  18. AAV-hSyn1-GCaMP6s-P2A-nls-dTomato (AAV Retrograde)

    Viral Prep
    #51084-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from AAV-hSyn1-GCaMP6s-P2A-nls-dTomato (#51084). In addition to the viral particles, you will also receive purified AAV-hSyn1-GCaMP6s-P2A-nls-dTomato plasmid DNA. GCaMP6s calcium sensor and bicistronic, physically separate nuclear localized dTomato expression under a human synapsin1 promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Jonathan Ting
    Promoter
    Syn
    Tags
    nuclear dTomato (physically separate, not a fusion protein)
    Available Since
    June 21, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 9761 - 9780 of 169450 results