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pRD324

Plasmid

#198045

Purpose

Expression of HMfB D14A K30A E34A in E. coli

Depositor

Remus Dame

Article

Erkelens et al Front Microbiol. 2023 Apr 18;14:1166608.

Insert

HMfB D14A K30A E34A

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCri-11b

Plasmid

#61333

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

His-tag and SUMO-tag

Expression

Bacterial

Available Since

April 1, 2015

Availability

Academic Institutions and Nonprofits only
pLXMA L2A

Plasmid

#153360

Purpose

Expression vector for plants with medium-copy number origin of replication

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Type

Empty backbone

Expression

Plant

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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TOPO-BC43

Plasmid

#84437

Purpose

Encodes illumina barcodes for allele frequency sequencing (FREQ-Seq-Squared)

Depositor

J.J. Emerson

Article

Zhao et al G3 (Bethesda). 2023 Sep 30;13(10):jkad16

Insert

BC43

Use

Barcoding plasmids

Promoter

SP6/T7

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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p286 TIR1_HA_hH11

Plasmid

#164853

Purpose

constitutive TIR1 expression vector for human H11 locus

Depositor

Philip Jordan

Article

Pryzhkova et al Stem Cell Res. 2020 Nov 5;49:102078. doi

Insert

OsTIR1

Tags

HA tag

Expression

Mammalian

Promoter

EF1-alpha

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMpGWB102-Citrine-NLS

Plasmid

#79136

Purpose

Binary vectors for expression of Citrine fluorescent protein with a nuclear localization signal in plants

Depositor

Yutaka Kodama

Article

Ogasawara et al Plant Cell Environ. 2013 Feb 20. doi: 10

Insert

Citrine-NLS

Tags

NLS (Nuclear Localization Signal)

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

Aug. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pMXLC_pFa

Plasmid

#114219

Purpose

POC12355, MetClo assembly vector with p15A replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type p-a

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMXLK_dFq

Plasmid

#114237

Purpose

POC12377, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type d-q

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pCR3.1-OPNc PSM5

Plasmid

#106330

Purpose

Mammalian expression of mutant osteopontin

Depositor

Georg Weber

Article

Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

Insert

OPNc (SPP1 Human)

Expression

Mammalian

Mutation

OPNc phosphorylation site mutant SGSSEEKQNAVSSEET…

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pRL2697

Plasmid

#70693

Purpose

pDU1-E. coli shuttle vector (Cm-r Em-r) with extended polylinker

Depositor

Peter Wolk

Article

Wolk et al Arch Microbiol. 2007 Dec;188(6):551-63.

Type

Empty backbone

Use

Bacterial shuttle vector

Available Since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-gap16-ClopHensor

Plasmid

#26006

Depositor

Daniele Arosio

Article

Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

Insert

gap16-ClopHensor

Tags

Gap16 and Strep-II

Expression

Mammalian

Mutation

N-term (16AA) of Gap-43

Available Since

Sept. 16, 2010

Availability

Academic Institutions and Nonprofits only

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pMXLK_pFa

Plasmid

#114229

Purpose

POC12367, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type p-a

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pOGG050

Plasmid

#113999

Purpose

SC module. Thermostable beta-galactosidase reporter

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

celB

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pOGG083

Plasmid

#114001

Purpose

SC module. Beta-glucosidase reporter

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

gusA

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMTB-Multibow-hR

Plasmid

#60997

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes nuclear dTomato

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

dTomato

Use

Zebrafish

Tags

histone 2B tag

Promoter

Bactin2

Available Since

March 9, 2015

Availability

Academic Institutions and Nonprofits only
pLIC.B3.AF2373

Plasmid

#11529

Depositor

Sung-Hou Kim

Article

Liu et al J Mol Biol. 2005 Nov 25. 354(2):289-303.

Insert

NAD kinase (AF2373 A. fulgidus)

Tags

HisTag, TEV cleavable

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pDest490-OPN-10 kDa

Plasmid

#17595

Depositor

Xin Wang

Article

Takafuji et al Oncogene. 2007 Sep 27. 26(44):6361-71.

Insert

OPN-10 kDa (SPP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Base pairs 499–630

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only
pDest490-OPN-CT

Plasmid

#17593

Depositor

Xin Wang

Article

Takafuji et al Oncogene. 2007 Sep 27. 26(44):6361-71.

Insert

OPN-CT (SPP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Base pairs 631–942

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only
pOGG039

Plasmid

#113996

Purpose

SC module. Terminator for T7RNAP

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Terminator for T7RNAP

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCri-4a

Plasmid

#61314

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

His-tag and TRX-tag

Expression

Bacterial

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only