We narrowed to 1,231 results for: POLI

1. pTK312

   Plasmid

   #59569

   Purpose

   TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak EGFP ochre attB2 AscI (truncated E1) 3'UTR Poly A I-SceI

   Depositor

   Ron Weiss

   Article

   Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

   Insert

   SGP XbaI attB1 Kozak EGFP ochre attB2 AscI
   

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMCSG28-SH3-588

   Plasmid

   #185920

   Purpose

   Expresses SH3-588, a designed poly-proline peptide recruiting catechol O-methyltransferase

   Depositor

   Brian Kuhlman

   Article

   Park et al Nat Chem Biol. 2023 Apr;19(4):460-467. d

   Insert

   SH3-588
   

   Tags

   6x HIS tag

   Expression

   Bacterial

   Mutation

   Designed enzyme with novel interface between huma…

   Promoter

   T7

   Available Since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pG23A

   Plasmid

   #102884

   Purpose

   Plasmid used for the C-terminal tagging of Cdc14 with mCherry and the auxin-inducible degron in yeast.

   Depositor

   Matthias Heinemann

   Article

   Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

   Insert

   Cdc14/mCherry-AID(71-114)-NatMX (CDC14 Budding Yeast)
   

   Tags

   AID(71-114) and mCherry

   Expression

   Yeast

   Promoter

   Native promoter

   Available Since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pK RagA(Q66L)-RagC

   Plasmid

   #99700

   Purpose

   Bacterial expression of RagA(Q66L)-RagC

   Depositor

   Kuang Shen, David Sabatini

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Inserts

   RagA (RRAGA Human)
   RagC (RRAGC Human)
   

   Tags

   His tag, Poly Arg, and SUMO

   Expression

   Bacterial

   Mutation

   L91P

   Promoter

   T7

   Available Since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. GLN, Linear-3, PolyU(1)

   Plasmid

   #166984

   Purpose

   Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination module:PolyU(1)
   

   Expression

   Mammalian

   Available Since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJEP223-AAV-EFS-GFP-pA

   Plasmid

   #62913

   Purpose

   AAV vector backbone designed to express GFP from a EFS promoter. It also contains a poly-Adenylation Signal (pA)

   Depositor

   Jonathan Ploski

   Article

   Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

   Insert

   Green Fluorescent protein (GFP)
   

   Use

   AAV

   Promoter

   short form of Elongation Factor1α (EFS)

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pK RagA-RagC

   Plasmid

   #99649

   Purpose

   Bacterial expression of wildtype Rag GTPase dimer

   Depositor

   Kuang Shen, David Sabatini

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Inserts

   RagA (RRAGA Human)
   RagC (RRAGC Human)
   

   Tags

   10x His tag, Poly arginine linker, and SUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBT100-neo

   Plasmid

   #123137

   Purpose

   Golden Gate donor vector for the assembly of EGFP-based mammalian recombinase reporters.

   Depositor

   David Liu

   Article

   Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

   Insert

   Neomycin, poly-A terminator
   

   Use

   Synthetic Biology; Golden gate donor vector

   Promoter

   none

   Available Since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pK RagA-RagC(Q120L)

   Plasmid

   #99701

   Purpose

   Bacterial expression of RagA-RagC(Q120L)

   Depositor

   Kuang Shen, David Sabatini

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Inserts

   RagA (RRAGA Human)
   RagC (RRAGC Human)
   

   Tags

   His tag, Poly Arg, and SUMO

   Expression

   Bacterial

   Mutation

   Q120L

   Promoter

   T7

   Available Since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmRNA-iEMCV-EGFP-A120

    Plasmid

    #225905

    Purpose

    In vitro transcription of cap-independently (EMCV IRES) translated mRNA encoding EGFP.

    Depositor

    Tom Brown

    Article

    Golojuch et al Nat Commun. 2025 Oct 16;16(1):9205. doi:

    Inserts

    A-inserted T7 class III Φ6.5 promoter
    EMCV IRES
    EGFP
    2x β-globin UTR
    poly(A) tail
    

    Use

    Synthetic Biology; Mrna preparation

    Expression

    Mammalian

    Available Since

    Oct. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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