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Plasmid#214918Purposeconstitutive expression of SRRD tagged with HADepositorInsertSRRD (SRRD Human)
UseLentiviralTagsHAExpressionMutationPromoterAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
p2T-CMV-miniSdd3-BE4max-BlastR
Plasmid#204849PurposeFor cytosine base editing using miniSdd3 in mammalian cellsDepositorInsertminiSdd3-nSpCas9(D10A)-UGI
UseCRISPRTagsExpressionMammalianMutationD10A in SpCas9PromoterCMV, SV40, EM7Available sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetP-HsEDC3_192-228-GST-HsRCK_E
Plasmid#146211PurposeBacterial Expression of HsEDC3_192-228-HsRckDepositorInsertHsEDC3_192-228-HsRck (EDC3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetP-HsEDC3_192-228-GST-HsRCK-E236Q_E
Plasmid#146212PurposeBacterial Expression of HsEDC3_192-228-HsRck-E236QDepositorInsertHsEDC3_192-228-HsRck-E236Q (EDC3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetP-HsEDC3_192-228-GST-HsRCK-K135A_E
Plasmid#146213PurposeBacterial Expression of HsEDC3_192-228-HsRck-K135ADepositorInsertHsEDC3_192-228-HsRck-K135A (EDC3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetP-HsEDC3_192-508-GST-HsRCK-E236Q_E
Plasmid#146215PurposeBacterial Expression of HsEDC3_192-508-HsRck-E236QDepositorInsertHsEDC3_192-508-HsRck-E236Q (EDC3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetP-HsEDC3_192-508-GST-HsRCK-K135A_E
Plasmid#146216PurposeBacterial Expression of HsEDC3_192-508-HsRck-K135ADepositorInsertHsEDC3_192-508-HsRck-K135A (EDC3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmMe31B-Q281AH284A_E
Plasmid#146204PurposeInsect Expression of DmMe31B-Q281AH284ADepositorInsertDmMe31B-Q281AH284A (me31B Fly)
UseTagsExpressionInsectMutationone silent mutation A1101G and two non silent mut…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmMe31B-T288AK292A_E
Plasmid#146205PurposeInsect Expression of DmMe31B-T288AK292ADepositorInsertDmMe31B-T288AK292A (me31B Fly)
UseTagsExpressionInsectMutationone silent mutation A1101G and two non silent mut…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
KTK_113
Plasmid#180540PurposeEntry vector containing Signal peptide region of CsgA including SecB-N22 sequencedDepositorInsertSignal peptide region of CsgA including SecB-N22 sequenced
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-mCherry-SynaptoZip
Plasmid#177318PurposeAAV expression of the synaptic activity-marker SynaptoZip fused to mCherry driven by human Synapsin promoterDepositorInsertmCherry-SynaptoZip (Vamp2 Rat)
UseAAVTagsMyc and mCherryExpressionMammalianMutationPromoterhSynAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetP-HsEDC3_192-508-GST-HsRCK_E
Plasmid#146214PurposeBacterial Expression of HsEDC3_192-508-HsRckDepositorInsertHsEDC3_192-508-HsRck (EDC3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGem-LP1-loxP-bar-lox2272-71
Plasmid#168785PurposeVector to create landing pad 1 (LP1) by homologous recombination in Aspergillus nidulans ΔSt site. The strain A. nidulans LP1 is used for recombinase mediated chromosomal integration.DepositorInsertsbar
Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
loxP
lox2272-71
UseCre/Lox and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGem-LP2-loxP-bar-lox2272-71
Plasmid#168786PurposeVector to create landing pad 2 (LP2) by homologous recombination in Aspergillus nidulans IS1 site. The strain A. nidulans LP2 is used for recombinase mediated chromosomal integration.DepositorInsertsbar
IS1 Homology arm 1
IS1 Homology arm 2
loxP
lox2272-71
UseCre/Lox and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
mEos2-dVHH[GFP] in pCSDEST
Plasmid#162870PurposeSuper-resolution of GFP in cultureDepositorInsertmEos2-dVHH[EGFP]
UseTagsmEos2ExpressionMammalianMutationPromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJV126
Plasmid#124250PurposeIn vitro transcription plasmid for T7 transcription of wildtype Her2 sequence targeted to the endosome. Linearize with SapI.DepositorInsertMinigene containing Her2 sequences fused to endosomal targeting sequence of class I MHC
UseIn vitro transcription templateTagsExpressionMutationPromoterT7Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJV127
Plasmid#124251PurposeIn vitro transcription plasmid for T7 transcription of Her2 ITD sequence targeted to the endosome. Linearize with SapI.DepositorInsertMinigene containing Her2 sequences fused to endosomal targeting sequence of class I MHC
UseIn vitro transcription templateTagsExpressionMutationPromoterT7Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJV128
Plasmid#124252PurposeIn vitro transcription plasmid for T7 transcription of KRAS WT sequence targeted to the endosome. Linearize with SapI.DepositorInsertMinigene containing KRAS sequences fused to endosomal targeting sequence of class I MHC
UseIn vitro transcription templateTagsExpressionMutationPromoterT7Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJV129
Plasmid#124253PurposeIn vitro transcription plasmid for T7 transcription of KRAS G12V sequence targeted to the endosome. Linearize with SapI.DepositorInsertMinigene containing KRAS sequences fused to endosomal targeting sequence of class I MHC
UseIn vitro transcription templateTagsExpressionMutationPromoterT7Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBI-CMV1-mir144~451-CMV2-mir1
Plasmid#164664PurposeExpress miR-144~451 and miR-1-1 in mammalian cellsDepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only