We narrowed to 12,099 results for: cel.2;

1. Ikaros-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

   Plasmid

   #75242

   Purpose

   CRISPR/Cas9 NICKASE plasmid against human Ikaros (1/2)

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   sgRNA against human Ikaros (IKZF1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLQ9834_sgBB: pHR-hU6-CasMINI sgRNA_#2; EF1a-Puro-T2A-BFP- WPRE

   Plasmid

   #180280

   Purpose

   The CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting new guide sequences.

   Depositor

   Stanley Qi

   Article

   Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

   Insert

   CasMINI sgRNA (backbone) and BFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-2/3-3'

   Plasmid

   #117325

   Purpose

   CRISPR-Cas9 for miR-124-2/3, 3'

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   gRNA miR-124-2/3-3' (MIR124-3 Synthetic)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-1/2-5'

   Plasmid

   #117322

   Purpose

   CRISPR-Cas9 for miR-124-1/2, 5'

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   gRNA miR-124-1/2-5' (MIR124-1 Synthetic)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPB-EF1A-HALO-hLRRK2 (LIR1+2 mutant WEVL -> AEVA / WTFI -> ATFA)

   Plasmid

   #229744

   Purpose

   Expresses Halo tagged human LRRK2 with LIR1 and LIR2 point mutations in mammalian cells. Stable expression with piggybac transposase.

   Depositor

   Shawn Ferguson

   Article

   Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   LIR1+2 mutant WEVL -> AEVA / WTFI -> ATFA

   Promoter

   EF1A

   Available since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. -962 human cyclin D1 promoter TCF (1)(2) sites mutant pGL3Basic

   Plasmid

   #32734

   Depositor

   Frank McCormick

   Article

   Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

   Insert

   CCND1 promoter (CCND1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   -75 TCF(1) and -68 TCF(2) - CTTTGATCTTTGCT deleti…

   Promoter

   -962 CCND1

   Available since

   Jan. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pETDuet1_BCL2L13(1-465aa)-THROMBIN-GST (Y213A/I216A/W276A/I279A; deltaLIR1+2)

   Plasmid

   #223752

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   BCL2L13 soluble part (BCL2L13 Human)
   

   Use

   Tags

   Thrombin cleavage-GST

   Expression

   Bacterial

   Mutation

   expresses aa 1-465, with Y213A/I216A/W276A/I279A;…

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. SKA974

   Bacterial Strain

   #80403

   Purpose

   JT2-based E. coli host strain for optogenetic control of cell growth.

   Depositor

   Mustafa Khammash

   Article

   Milias-Argeitis et al Nat Commun. 2016 Aug 26;7:12546. doi: 10

   Bacterial Resistance

   Kanamycin

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pACAGW-ChR2-Venus-AAV (AAV1)

   Viral Prep

   #20071-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pACAGW-ChR2-Venus-AAV (#20071). In addition to the viral particles, you will also receive purified pACAGW-ChR2-Venus-AAV plasmid DNA. CAG-driven, channelrhodopsin fused to Venus for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karel Svoboda

   Article

   Leopoldo et al Nature. 2009 Jan 18.

   Promoter

   CAGG

   Tags

   Venus

   Available since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

10. pACAGW-ChR2-Venus-AAV (AAV9)

    Viral Prep

    #20071-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pACAGW-ChR2-Venus-AAV (#20071). In addition to the viral particles, you will also receive purified pACAGW-ChR2-Venus-AAV plasmid DNA. CAG-driven, channelrhodopsin fused to Venus for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karel Svoboda

    Article

    Leopoldo et al Nature. 2009 Jan 18.

    Promoter

    CAGG

    Tags

    Venus

    Available since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

11. Thy-1-CRISPR-gRNA#2-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

    Plasmid

    #124284

    Purpose

    sgRNA against human Thy-1

    Depositor

    Katalin Josvay, Csaba Vizler

    Insert

    Homo sapiens Thy-1 cell surface antigen (THY1), transcript variant 1 (THY1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

12. pAV-U6+27-Tornado-F30-Broccoli-Squash-SAM-sensor 4-2

    Plasmid

    #177919

    Purpose

    Expresses ratiometric Squash-SAM sensor 4-2 as circular RNA in mammalian cells using the Tornado expression system.

    Depositor

    Samie Jaffrey

    Article

    Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

    Insert

    Tornado-F30-Broccoli-Squash-SAM-sensor 4-2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6+27

    Available since

    Feb. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. TL1 alphaDdCBE A1 (DddA6-C) - TL1 alphaL_DdCBE 2 (left, DddA11-C)

    Plasmid

    #221994

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE A1 repeats-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    TALE: Q231R, W232G, S233A | DddA: T1413I

    Promoter

    CMV

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSLQ9834_sglacZ: pHR-hU6-CasMINI sgRNA_#2 (sgLacZ); EF1a-Puro-T2A-BFP- WPRE

    Plasmid

    #176272

    Purpose

    Expression of non-targeting sgRNA of CasMINI

    Depositor

    Stanley Qi

    Article

    Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

    Insert

    CasMINI non-targeting sgRNA (sgLacZ) and BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSLQ9834_sgTet: pHR-hU6-CasMINI sgRNA_#2 (sgTet); EF1a-Puro-T2A-BFP- WPRE

    Plasmid

    #176273

    Purpose

    Expression of CasMINI sgRNA targeting TRE3G promoter in dCasMINI-mediated GFP activation assays.

    Depositor

    Stanley Qi

    Article

    Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

    Insert

    CasMINI sgRNA (sgTet) and BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTM-RBDv2

    Plasmid

    #162785

    Purpose

    SARS-CoV-2 S protein receptor binding domain (RBD) expression in mammalian cells

    Depositor

    Ivan Vorobiev

    Article

    Sinegubova et al PLoS One. 2021 Feb 2;16(2):e0242890. doi

    Insert

    SARS-CoV-2 S protein receptor binding domain (RBD) (S Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2))
    

    Use

    Tags

    10xHis, c-myc, and hTPA leader

    Expression

    Mammalian

    Mutation

    Promoter

    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

    Available since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (Y213A/I216A/W276A/I279A; deltaLIR1+2)

    Plasmid

    #223782

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    BCL2L13 soluble part (BCL2L13 Human)
    

    Use

    Tags

    EGFP-TEV-GST

    Expression

    Bacterial

    Mutation

    Y213A/I216A/W276A/I279A; deletion of LIR1+2

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFastBac1-Nucleocapsid

    Plasmid

    #179696

    Purpose

    Expresses H6 tagged SARS CoV-2 nucleocapsid in Sf9 cells

    Depositor

    Mark Arbing

    Insert

    SARS-CoV-2 Nucleocapsid (N protein) (N Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    His6-TEV protease site

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Jan. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. Venus-Bcl2-pEGFP-C1

    Plasmid

    #177417

    Purpose

    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bcl-2.

    Depositor

    David Andrews

    Article

    Aranovich et al Mol Cell. 2012 Mar 30;45(6):754-63. doi:

    Insert

    Bcl-2 (BCL2 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Flag-Bcl2-Maob

    Plasmid

    #18005

    Depositor

    Clark Distelhorst

    Article

    Wang et al J Biol Chem. 2001 Nov 23. 276(47):44117-

    Insert

    Mitochondria-targeted Bcl-2 (BCL2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    C terminal of Bcl-2 replaced with Maob. Targets B…

    Promoter

    CMV

    Available since

    June 4, 2008

    Availability

    Academic Institutions and Nonprofits only